Co-culture of gastric organoids and immortalized stomach mesenchymal cells

Methods in Molecular Biology

Volumn 1422, Issue , 2016, Pages 23-31

  Bertaux Skeirik, Nina ^a   Centeno, Jomaris ^a   Feng, Rui ^a   Schumacher, Michael A ^a   Shivdasani, Ramesh A ^a   Zavros, Yana ^a  

^a NONE

Author keywords

Co culture; Immortalized stomach mesenchymal cells; Mouse fundic gastric organoids

Indexed keywords

ABDOMINAL CAVITY; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL ADHESION; CELL PROLIFERATION; COCULTURE; CYTOPLASM; HELICOBACTER PYLORI; IMMORTALIZED STOMACH MESENCHYMAL CELL; MESENCHYME CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PYLORIC SPHINCTER; ROOM TEMPERATURE; STOMACH; STOMACH GLAND; ANIMAL; CELL CULTURE; CYTOLOGY; EPITHELIUM CELL; MESENCHYMAL STROMA CELL; METABOLISM; ORGANOID; PROCEDURES; STOMACH MUCOSA;

BIOLOGICAL MARKER;

ANIMALS; BIOMARKERS; CELL PROLIFERATION; CELLS, CULTURED; COCULTURE TECHNIQUES; EPITHELIAL CELLS; GASTRIC MUCOSA; MESENCHYMAL STROMAL CELLS; MICE; ORGANOIDS;

EID: 84990057354     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3603-8_3     Document Type: Article

References (15)

1. Huch M, Dorrell C, Boj SF et al (2013) In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature 494:247-250
2. Skardal A, Devarasetty M, Rodman C, Atala A, Soker S et al (2015) Liver-tumor hybrid organoids for modeling tumor growth and drug response in vitro. Ann Biomed Eng 43:2361-2373
3. Huch M, Bonfanti P, Boj SF et al (2013) Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis. EMBO J 32:2708-2721
4. Sato T, Stange DE, Ferrante M et al (2011) Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium. Gastroenterology 141:1762-1772
5. Mahe MM, Aihara E, Schumacher MA et al (2013) Establishment of gastrointestinal epithelial organoids. Curr Protoc Mouse Biol 3:217-240
6. Barker N, Huch M, Kujala P et al (2010) Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro. Cell Stem Cell 6:25-36
7. Schumacher MA, Aihara E, Feng R et al (2015) The use of murine-derived fundic organoids in studies of gastric physiology. J Physiol 593:1809-1827
8. Bertaux-Skeirik N, Feng R, Schumacher MA et al (2015) CD44 plays a functional role in helicobacter pylori-induced epithelial cell proliferation. PLoS Pathog 11:e1004663
9. Wroblewski LE, Piazuelo MB, Chaturvedi R et al (2015) Helicobacter pylori targets cancerassociated apical-junctional constituents in gastroids and gastric epithelial cells. Gut 64:720-730
10. Sato T, Vries RG, Snippert HJ et al (2009) Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459:262-265
11. Jung P, Sato T, Barriga FM et al (2011) Isolation and in vitro expansion of human colonic stem cells. Nat Med 17:1225-1227
12. Schumacher MA, Feng R, Aihara E et al (2015) Helicobacter pylori-induced Sonic Hedgehog expression is regulated by NFκB pathway activation: the use of a novel in vitro model to study epithelial response to infection. Helicobacter 20:19-28
13. Feng R, Aihara E, Kenny S et al (2014) Indian Hedgehog mediates gastrin-induced proliferation in stomach of adult mice. Gastroenterology 147:655-666
14. Stange DE, Koo BK, Huch M et al (2013) Differentiated Troy + chief cells act as reserve stem cells to generate all lineages of the stomach epithelium. Cell 155:357-368
15. Jayewickreme CD, Shivdasani RA (2015) Control of stomach smooth muscle development and intestinal rotation by transcription factor BARX1. Dev Biol 405:21-32