Sequencing and cloning of antigen-specific antibodies from mouse memory B cells

Nature Protocols

Volumn 11, Issue 10, 2016, Pages 1908-1923

  Von Boehmer, Lotta ^a   Liu, Cassie ^a   Ackerman, Sarah ^a   Gitlin, Alexander D ^a   Wang, Qiao ^a   Gazumyan, Anna ^a,b   Nussenzweig, Michel C ^a,b  

^a ROCKEFELLER UNIVERSITY   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; ANTIGEN SPECIFIC ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY PRODUCTION; ANTIGEN ANTIBODY COMPLEX; ARTICLE; CELL SUSPENSION; CLINICAL PROTOCOL; CONTROLLED STUDY; EXPRESSION VECTOR; FLUORESCENCE ACTIVATED CELL SORTING; GENE SEQUENCE; GENETIC TRANSCRIPTION; IN VITRO STUDY; MEMORY CELL; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PURIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SEQUENCE AND LIGATION INDEPENDENT CLONING METHOD;

EID: 84988632777     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.102     Document Type: Article

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