Suppression of T Cell Autophagy Results in Decreased Viability and Function of T Cells Through Accelerated Apoptosis in a Murine Sepsis Model

Critical Care Medicine

Volumn 45, Issue 1, 2017, Pages e77-e85

  Oami, Takehiko ^a   Watanabe, Eizo ^a   Hatano, Masahiko ^b   Sunahara, Satoshi ^a   Fujimura, Lisa ^b   Sakamoto, Akemi ^b   Ito, Chizuru ^a   Toshimori, Kiyotaka ^a   Oda, Shigeto ^a  

^a CHIBA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b CHIBA UNIVERSITY   (Japan)

Author keywords

cecal ligation and puncture; cell death; critical care; immunosuppression

Indexed keywords

GREEN FLUORESCENT PROTEIN; INTERLEUKIN 10; PROTEIN BCL 2; PROTEIN P62; BIM PROTEIN; CD274 PROTEIN, MOUSE; PROGRAMMED DEATH 1 LIGAND 1;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; AUTOPHAGOSOME; AUTOPHAGY; BIOACCUMULATION; CD4+ T LYMPHOCYTE; CECAL LIGATION AND PUNCTURE-INDUCED SEPSIS; CELL VIABILITY; CONTROLLED STUDY; CYTOKINE PRODUCTION; FLOW CYTOMETRY; GENE EXPRESSION; HOMEOSTASIS; MALE; MOUSE; MOUSE MODEL; NONHUMAN; PRIORITY JOURNAL; SPLEEN; SPLEEN CELL; SURVIVAL RATE; ANIMAL; CECUM; CELL SURVIVAL; CYTOLOGY; DISEASE MODEL; IMMUNOLOGY; KNOCKOUT MOUSE; METABOLISM; MITOCHONDRION; MORTALITY; SEPSIS; SURGERY; T LYMPHOCYTE; TRANSGENIC MOUSE;

ANIMALS; ANTIGENS, CD274; APOPTOSIS; AUTOPHAGY; BCL-2-LIKE PROTEIN 11; CECUM; CELL SURVIVAL; DISEASE MODELS, ANIMAL; INTERLEUKIN-10; MALE; MICE, KNOCKOUT; MICE, TRANSGENIC; MITOCHONDRIA; SEPSIS; SPLEEN; T-LYMPHOCYTES;

EID: 84987642363     PISSN: 00903493     EISSN: 15300293     Source Type: Journal    
DOI: 10.1097/CCM.0000000000002016     Document Type: Article

References (24)

1. Jawad I, Lukšić I, Rafnsson SB: Assessing available information on the burden of sepsis: Global estimates of incidence, prevalence and mortality. J Glob Health 2012; 2:010404
2. Nesseler N, Defontaine A, Launey Y, et al: Long-term mortality and quality of life after septic shock: A follow-up observational study. Intensive Care Med 2013; 39:881-888
3. Gentile LF, Cuenca AG, Efron PA, et al: Persistent infammation and immunosuppression: A common syndrome and new horizon for surgical intensive care. J Trauma Acute Care Surg 2012; 72:1491-1501
4. Otto G P, Sossdorf M, Claus RA, et al: The late phase of sepsis is characterized by an increased microbiological burden and death rate. Crit Care 2011; 15:R183
5. Hotchkiss RS, Monneret G, Payen D: Immunosuppression in sepsis: A novel understanding of the disorder and a new therapeutic approach. Lancet Infect Dis 2013; 13:260-268
6. Hotchkiss RS, Monneret G, Payen D: Sepsis-induced immunosup-pression: From cellular dysfunctions to immunotherapy. Nat Rev Immunol 2013; 13:862-874
7. Brahmamdam P, Inoue S, Unsinger J, et al: Delayed administration of anti-PD-1 antibody reverses immune dysfunction and improves survival during sepsis. J Leukoc Biol 2010; 88:233-240
8. Inoue S, Unsinger J, Davis CG, et al: IL-15 prevents apoptosis, reverses innate and adaptive immune dysfunction, and improves survival in sepsis. J Immunol 2010; 184:1401-1409
9. Shindo Y, Unsinger J, Burnham CA, et al: Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression. Shock 2015; 43:334-343
10. Hotchkiss RS, Sherwood ER: Immunology. Getting sepsis therapy right. Science 2015; 347:1201-1202
11. Hotchkiss RS, Strasser A, McDunn JE, et al: Cell death. N Engl J Med 2009; 361:1570-1583
12. Choi AM, Ryter SW, Levine B: Autophagy in human health and disease. N Engl J Med 2013; 368:651-662
13. Takahashi W, Watanabe E, Fujimura L, et al: Kinetics and protective role of autophagy in a mouse cecal ligation and puncture-induced sepsis. Crit Care 2013; 17:R160
14. Howell GM, Gomez H, Collage RD, et al: Augmenting autophagy to treat acute kidney injury during endotoxemia in mice. PLoS One 2013; 8:e69520
15. Hsieh CH, Pai PY, Hsueh HW, et al: Complete induction of autophagy is essential for cardioprotection in sepsis. Ann Surg 2011; 253:1190-1200
16. Lin CW, Lo S, Hsu C, et al: T-cell autophagy defciency increases mortality and suppresses immune responses after sepsis. PLoS One 2014; 9:e102066
17. Crouser ED, Julian MW, Huff JE, et al: Carbamoyl phosphate syn-thase-1: A marker of mitochondrial damage and depletion in the liver during sepsis. Crit Care Med 2006; 34:2439-2446
18. Watanabe E, Muenzer JT, Hawkins WG, et al: Sepsis induces extensive autophagic vacuolization in hepatocytes: A clinical and laboratory-based study. Lab Invest 2009; 89:549-561
19. Shvets E, Fass E, Elazar Z: Utilizing fow cytometry to monitor autophagy in living mammalian cells. Autophagy 2008; 4:621-628
20. Jia W, He YW: Temporal regulation of intracellular organelle homeostasis in T lymphocytes by autophagy. J Immunol 2011; 186:5313-5322
21. Pua HH, Guo J, Komatsu M, et al: Autophagy is essential for mitochondrial clearance in mature T lymphocytes. J Immunol 2009; 182:4046-4055
22. Hubbard VM, Valdor R, Patel B, et al: Macroautophagy regulates energy metabolism during effector T cell activation. J Immunol 2010; 185:7349-7357
23. Saitoh T, Fujita N, Jang MH, et al: Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature 2008; 456:264-268
24. Peral de Castro C, Jones SA, Ni Cheallaigh C, et al: Autophagy regulates IL-23 secretion and innate T cell responses through effects on IL-1 secretion. J Immunol 2012; 189:4144-4153