Molecular variation analysis of Aspergillus flavus using polymerase chain reaction‑restriction fragment length polymorphism of the internal transcribed spacer rDNA region

Experimental and Therapeutic Medicine

Volumn 12, Issue 3, 2016, Pages 1628-1632

  Zarrin, Majid ^a   Erfaninejad, Maryam ^a  

^a AHVAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Aspergillus flavus; Internal transcribed spacer rDNA region; Polymerase chain reaction restriction fragment length polymorphism

Indexed keywords

INTERNAL TRANSCRIBED SPACER;

ARTICLE; ASPERGILLUS FLAVUS; DNA EXTRACTION; GENE AMPLIFICATION; GENETIC ANALYSIS; MOLECULAR VARIATION ANALYSIS; MYCELIUM; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RESTRICTION SITE;

EID: 84980410144     PISSN: 17920981     EISSN: 17921015     Source Type: Journal    
DOI: 10.3892/etm.2016.3479     Document Type: Article

References (24)

1. Krishnan S, Manavathu EK and Chandrasekar PH: Aspergillus flavus: An emerging non-fumigatus Aspergillus species of significance. Mycoses 52: 206-222, 2009.
2. Pasqualotto AC: Differences in pathogenicity and clinical syndromes due to Aspergillus fumigatus and Aspergillus flavus. Med Mycol 47 (Suppl 1): S261-S270, 2009.
3. Ehrlich KC and Mack BM: Comparison of Expression of Secondary Metabolite Biosynthesis Cluster Genes in Aspergillus flavus, A. parasiticus, and A. oryzae. Toxins (Basel) 6: 1916-1928, 2014.
4. Karthikeyan M, Sandosskumar R, Mathiyazhagan S, Mohankumar M, Valluvaparidasan V, Kumar S and Velazhahan R: Genetic variability and aflatoxigenic potential of Aspergillus flavus isolates from maize. Arch Phytopathol Plant Protect 42: 83-91, 2009.
5. Zhang ZG, Zhang JY, Zheng XB, Yang YW and Ko WH: Molecular distinctions between Phytophthora capsici and P. tropicalis based on ITS sequences of ribosomal DNA. J Phytopathol 152: 358-364, 2004.
6. Khoodoo MHR and Jaufeerally-Fakim Y: RAPD-PCR fingerprinting and southern analysis of Xanthomonas axonopodis pv. Dieffenbachiae strains isolated from different aroid hosts and locations. Plant Dis 88: 980-988, 2004.
7. Henry T, Iwen PC and Hinrichs SH: Identification of Aspergillus species using internal transcribed spacer regions 1 and 2. J Clin Microbiol 38: 1510-1515, 2000.
8. Gomes EA, Maria Kasuya CM, de Barros EG, Borges AC and Araújo EF: Polymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 26 isolates of ectomycorrhizal fungi. Genet Mol Biol 25: 477-483, 2002.
9. Krimitzas A, Pyrri I, Kouvelis VN, Kapsanaki-Gotsi E and Typas MA: A phylogenetic analysis of Greek isolates of Aspergillus species based on morphology and nuclear and mitochondrial gene sequences. Biomed Res Int 2013: 260395, 2013.
10. Criseo G, Bagnara A and Bisignano G: Differentiation of aflatoxin producing and non-producing strains of Aspergillus flavus group. Lett Appl Microbiol 33: 291-295, 2001.
11. Chen RS, Tsay JG, Huang YF and Chiou RY: Polymerase chain reaction-mediated characterization of molds belonging to the Aspergillus flavus group and detection of Aspergillus parasiticus in peanut kernels by multiplex polymerase chain reaction. J Food Prot 65: 840-844, 2002.
12. Toju H, Tanabe AS, Yamamoto S and Sato H: High-coverage ITS primers for the DNA-based identification of ascomycetes and basidiomycetes in environmental samples. PLoS One 7: e40863, 2012.
13. Diguta CF, Vincent B, Guilloux-Benatier M, Alexandre H and Rousseaux S: PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes. Food Microbiol 28: 1145-1154, 2011.
14. Pereira F, Carneiro J and Amorim A: Identification of species with DNA-based technology: Current progress and challenges. Recent Pat DNA Gene Seq 2: 187-199, 2008.
15. Huang A, Li JW, Shen ZQ, Wang XW and Jin M: High-throughput identification of clinical pathogenic fungi by hybridization to an oligonucleotide microarray. J Clin Microbiol 44: 3299-3305, 2006.
16. Nicholson P, Simpson DR, Weston G, Rezanoor HN, Lees AK, Parry DW and Joyce D: Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiol Mol Plant Pathol 53: 17-37, 1998.
17. O’Donnell K, Cigelnik E and Nirenberg HI: Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90: 465-493, 1998.
18. Paterson RRM: Identification and quantification of mycotoxigenic fungi by PCR. Process Biochem 71: 1467-1474, 2006.
19. Ferrer C, Colom F, Frasés S, Mulet E, Abad JL and Alió JL: Detection and identification of fungal pathogens by PCR and by ITS2 and 5.8S ribosomal DNA typing in ocular infections. J Clin Microbiol 39: 2873-2879, 2001.
20. Mirete S, Patiño B, Vázquez C, Jiménez M, Hinojo MJ, Soldevilla C and González-Jaén MT: Fumonisin production by Gibberella fujikuroi strains from Pinus species. Int J Food Microbiol 89: 213-221, 2003.
21. González-Salgado A, Patño B, Vázquez C and González-Jaén MT: Discrimination of Aspergillus niger and other Aspergillus species belonging to section Nigri by PCR assays. FEMS Microbiol Lett 245: 353-361, 2005.
22. Kizis D, Natskoulis P, Nychas GJ and Panagou EZ: Biodiversity and ITS-RFLP characterisation of Aspergillus section Nigri isolates in grapes from four traditional grape-producing areas in Greece. PLoS One 9: e93923, 2014.
23. Dubey SC, Tripathi A and Singh SR: ITS-RFLP fingerprinting and molecular marker for detection of Fusarium oxysporum f.sp. ciceris. Folia Microbiol (Praha) 55: 629-634, 2010.
24. Mohankumar M, Vijayasamundeeswari A, Karthikeyan M, Mathiyazhagan S, Paranidharan V and Velazhahan R: Analysis of molecular variability among isolates of Aspergillus flavus by PCR-RFLP of the its regions of rDNA. J Plant Prot Res 50: 446-451, 2010.