Studying extracellular vesicle transfer by a Cre-loxP method

Nature Protocols

Volumn 11, Issue 1, 2016, Pages 87-101

  Zomer, Anoek ^a   Steenbeek, Sander Christiaan ^a   Maynard, Carrie ^a   Van Rheenen, Jacco ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY AFFINITY; ARTICLE; CONTROLLED STUDY; CRE LOXP SYSTEM; EXOSOME; EXPERIMENTAL DESIGN; FILTRATION; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENETIC RECOMBINATION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ULTRACENTRIFUGATION; WESTERN BLOTTING; ANIMAL; FEMALE; GENETIC PROCEDURES; GENETICS; HEK293 CELL LINE; METABOLISM; TRANSPORT AT THE CELLULAR LEVEL;

BACTERIOPHAGE RECEPTOR; CRE RECOMBINASE; INTEGRASE;

ANIMALS; ATTACHMENT SITES, MICROBIOLOGICAL; BIOLOGICAL TRANSPORT; EXTRACELLULAR VESICLES; FEMALE; GENETIC TECHNIQUES; HEK293 CELLS; HUMANS; INTEGRASES; MICE;

EID: 84979855846     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2015.138     Document Type: Article

References (39)

1. Ratajczak, J. et al. Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery. Leukemia 20, 847-856 (2006).
2. Valadi, H. et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat. Cell Biol. 9, 654-659 (2007).
3. Pegtel, D.M. et al. Functional delivery of viral miRNAs via exosomes. Proc. Natl. Acad. Sci. USA 107, 6328-6333 (2010).
4. Hood, J.L., San, R.S. & Wickline, S.A. Exosomes released by melanoma cells prepare sentinel lymph nodes for tumor metastasis. Cancer Res. 71, 3792-3801 (2011).
5. Costa-Silva, B. et al. Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver. Nat. Cell Biol. 17, 816-826 (2015).
6. Peinado, H. et al. Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Nat. Med. 18, 883-891 (2012).
7. Cai, Z. et al. Activated T cell exosomes promote tumor invasion via Fas signaling pathway. J. Immunol. 188, 5954-5961 (2012).
8. Luga, V. et al. Exosomes mediate stromal mobilization of autocrine Wnt-PCP signaling in breast cancer cell migration. Cell 151, 1542-1556 (2012).
9. Salomon, C. et al. Hypoxia-induced changes in the bioactivity of cytotrophoblast-derived exosomes. PLoS ONE 8, e79636 (2013).
10. Boelens, M.C. et al. Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways. Cell 159, 499-513 (2014).
11. Melo, S.A. et al. Cancer exosomes perform cell-independent microRNA biogenesis and promote tumorigenesis. Cancer Cell 26, 707-721 (2014).
12. Zomer, A. et al. In vivo imaging reveals extracellular vesicle-mediated phenocopying of metastatic behavior. Cell 161, 1046-1057 (2015).
13. Raposo, G. & Stoorvogel, W. Extracellular vesicles: exosomes, microvesicles, and friends. J. Cell Biol. 200, 373-383 (2013).
14. Thery, C. Exosomes: secreted vesicles and intercellular communications. F1000 Biol. Rep. 3, 15 (2011).
15. Colombo, M., Raposo, G. & Thery, C. Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu. Rev. Cell Dev. Biol. 30, 255-289 (2014).
16. Minciacchi, V.R., Freeman, M.R. & Di Vizio, D. Extracellular vesicles in cancer: exosomes, microvesicles and the emerging role of large oncosomes. Semin. Cell Dev. Biol. 40, 41-51 (2015).
17. Sverdlov, E.D. Amedeo Avogadro's cry: what is 1 μg of exosomes? Bioessays 34, 873-875 (2012).
18. Ridder, K. et al. Extracellular vesicle-mediated transfer of genetic information between the hematopoietic system and the brain in response to inflammation. PLoS Biol. 12, e1001874 (2014).
19. Ridder, K. et al. Extracellular vesicle-mediated transfer of functional RNA in the tumor microenvironment. Oncoimmunology 4, e1008371 (2015).
20. Thery, C., Amigorena, S., Raposo, G. & Clayton, A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr. Protoc. Cell Biol. Chapter 3 Unit 3.22 (2006).
21. van der Pol, E., Boing, A.N., Harrison, P., Sturk, A. & Nieuwland, R. Classification, functions, and clinical relevance of extracellular vesicles. Pharmacol. Rev. 64, 676-705 (2012).
22. Bard, M.P. et al. Proteomic analysis of exosomes isolated from human malignant pleural effusions. Am. J. Respir. Cell Mol. Biol. 31, 114-121 (2004).
23. Gyorgy, B. et al. Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. Blood 117, e39-e48 (2011).
24. Rood, I.M. et al. Comparison of three methods for isolation of urinary microvesicles to identify biomarkers of nephrotic syndrome. Kidney Int. 78, 810-816 (2010).
25. Gyorgy, B. et al. Membrane vesicles, current state-of-The-Art: emerging role of extracellular vesicles. Cell. Mol. Life Sci. 68, 2667-2688 (2011).
26. Lotvall, J. et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: A position statement from the International Society for Extracellular Vesicles. J. Extracell. Vesicles 3, 26913 (2014).
27. Christianson, H.C., Svensson, K.J., van Kuppevelt, T.H., Li, J.P. & Belting, M. Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity. Proc. Natl. Acad. Sci. USA 110, 17380-17385 (2013).
28. Svensson, K.J. et al. Exosome uptake depends on ERK1/2-heat shock protein 27 signaling and lipid raft-mediated endocytosis negatively regulated by caveolin-1. J. Biol. Chem. 288, 17713-17724 (2013).
29. Lai, C.P. et al. Visualization and tracking of tumour extracellular vesicle delivery and RNA translation using multiplexed reporters. Nat. Commun. 6, 7029 (2015).
30. Hoshino, D. et al. Exosome secretion is enhanced by invadopodia and drives invasive behavior. Cell Rep. 5, 1159-1168 (2013).
31. Suetsugu, A. et al. Imaging exosome transfer from breast cancer cells to stroma at metastatic sites in orthotopic nude-mouse models. Adv. Drug Deliv. Rev. 65, 383-390 (2013).
32. Sung, B.H., Ketova, T., Hoshino, D., Zijlstra, A. & Weaver, A.M. Directional cell movement through tissues is controlled by exosome secretion. Nat. Commun. 6, 7164 (2015).
33. Bobrie, A. et al. Rab27a supports exosome-dependent and-independent mechanisms that modify the tumor microenvironment and can promote tumor progression. Cancer Res. 72, 4920-4930 (2012).
34. Mulcahy, L.A., Pink, R.C. & Carter, D.R. Routes and mechanisms of extracellular vesicle uptake. J. Extracell. Vesicles 3, doi:10.3402/jev.v3.24641 (2014).
35. Vojtech, L. et al. Exosomes in human semen carry a distinctive repertoire of small non-coding RNAs with potential regulatory functions. Nucleic Acids Res. 42, 7290-7304 (2014).
36. Zomer, A. et al. Exosomes: fit to deliver small RNA. Commun. Integr. Biol. 3, 447-450 (2010).
37. Bolukbasi, M.F. et al. miR-1289 and 'zipcode'-like sequence enrich mRNAs in microvesicles. Mol. Ther. Nucleic Acids 1, e10 (2012).
38. Nordin, J.Z. et al. Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. Nanomedicine 11, 879-883 (2015).
39. Madisen, L. et al. A robust and high-Throughput Cre reporting and characterization system for the whole mouse brain. Nat. Neurosci. 13, 133-140 (2010).