Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

Nanomedicine: Nanotechnology, Biology, and Medicine

Volumn 11, Issue 4, 2015, Pages 879-883

  Nordin, Joel Z ^a   Lee, Yi ^b   Vader, Pieter ^b   Mäger, Imre ^b,c   Johansson, Henrik J ^a   Heusermann, Wolf ^d   Wiklander, Oscar P B ^a   Hällbrink, Mattias ^e   Seow, Yiqi ^f   Bultema, Jarred J ^a   Gilthorpe, Jonathan ^g   Davies, Tim ^h   Fairchild, Paul J ^h   Gabrielsson, Susanne ^a   Meisner Kober, Nicole C ^d   Lehtiö, Janne ^a   Smith, C I Edvard ^a   Wood, Matthew J A ^b   Andaloussi, Samir EL ^a,b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF TARTU   (Estonia)

^d NOVARTIS PHARMA AG   (Switzerland)

^e STOCKHOLM UNIVERSITY   (Sweden)

^f AGENCY FOR SCIENCE   (Singapore)

^g UMEÅ UNIVERSITY   (Sweden)

^h UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Biophysical properties; Extracellular vesicles; Size exclusion liquid chromatography; Ultracentrifugation; Ultrafiltration

Indexed keywords

BIOPHYSICS; CENTRIFUGATION; LIQUIDS; PROTEINS; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY; STEM CELLS; ULTRAFILTRATION;

BIOLOGICAL FLUIDS; BIOPHYSICAL PROPERTIES; CLINICAL APPLICATION; EXTRACELLULAR; FUNCTIONAL PROPERTIES; SIZE EXCLUSION; THERAPEUTIC AGENTS; ULTRACENTRIFUGATION;

LIQUID CHROMATOGRAPHY;

ANIMAL CELL; ARTICLE; BIOPHYSICS; CELL FUNCTION; CELL ISOLATION; CONTROLLED STUDY; EXOSOME; FLUORESCENCE CORRELATION SPECTROSCOPY; FLUORESCENCE MICROSCOPY; GEL PERMEATION CHROMATOGRAPHY; HYDRODYNAMICS; LIQUID CHROMATOGRAPHY; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; TRANSMISSION ELECTRON MICROSCOPY; ULTRACENTRIFUGATION; ULTRAFILTRATION; CHEMISTRY; HEK293 CELL LINE; HUMAN; MEMBRANE MICROPARTICLE; SIZE EXCLUSION CHROMATOGRAPHY; ULTRASTRUCTURE;

CELL-DERIVED MICROPARTICLES; CHROMATOGRAPHY, GEL; HEK293 CELLS; HUMANS; ULTRAFILTRATION;

EID: 84929380225     PISSN: 15499634     EISSN: 15499642     Source Type: Journal    
DOI: 10.1016/j.nano.2015.01.003     Document Type: Article

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