Inhibition of RhoA expression by adenovirus-mediated siRNA combined with TNF-α induced apoptosis of hepatocarcinoma cells

Bio-Medical Materials and Engineering

Volumn 26, Issue , 2015, Pages S2055-S2067

  Yin, Kun ^a   Zhao, Guihua ^a   Huang, Xiaodan ^a   Gao, Ge ^a   Sun, Hui ^a   Wei, Qingkuan ^a   Liu, Qiaoqiao ^a   Li, Mian ^a   Xu, Chao ^a   Zhu, Song ^a   Ba, Zaihua ^b   Yan, Ge ^a  

^a SHANDONG PROVINCIAL HOSPITAL AFFILIATED TO SHANDONG FIRST MEDICAL UNIVERSITY   (China)

^b JINING MEDICAL UNIVERSITY   (China)

Author keywords

adenovirus mediated siRNA; apoptosis; hepatocarcinoma cells; RhoA; tumor necrosis factor alpha

Indexed keywords

CELL DEATH; CELLS; DIAGNOSIS; DISEASES; GLYCOPROTEINS; MACROPHAGES; PROTEINS; TUMORS;

ADENOVIRUS-MEDIATED SIRNA; HEPATOCARCINOMA; HEPATOCELLULAR CARCINOMA; PROLIFERATION AND APOPTOSIS; RECOMBINANT ADENOVIRUS; RHOA; THERAPEUTIC EFFECTS; TUMOR NECROSIS FACTOR ALPHA;

CYTOLOGY;

ADENOVIRUS VECTOR; MESSENGER RNA; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; SMALL INTERFERING RNA; TUMOR NECROSIS FACTOR; TUMOR NECROSIS FACTOR ALPHA;

APOPTOSIS; ARTICLE; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; FREEZE THAWING; HEP-G2 CELL LINE; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IN VITRO GENE TRANSFER; INVERTED REPEAT; LIVER CELL CARCINOMA; MTT ASSAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECOMBINANT PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SHUTTLE VECTOR; TUNEL ASSAY; VIRUS PURIFICATION; WESTERN BLOTTING; ADENOVIRIDAE; CARCINOMA, HEPATOCELLULAR; GENETICS; HEPG2 CELL LINE; LIVER; LIVER NEOPLASMS; METABOLISM; PATHOLOGY; RNAI THERAPEUTICS;

ADENOVIRIDAE; APOPTOSIS; CARCINOMA, HEPATOCELLULAR; HEP G2 CELLS; HUMANS; LIVER; LIVER NEOPLASMS; RHOA GTP-BINDING PROTEIN; RNA, SMALL INTERFERING; RNAI THERAPEUTICS; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84979097481     PISSN: 09592989     EISSN: 18783619     Source Type: Journal    
DOI: 10.3233/BME-151511     Document Type: Article

References (37)

1. F. Balkwill. Tumour necrosis factor and cancer. Nature Reviews Cancer 9(2009), 361-371.
2. E. Kakiashvili, Q. Dan, M. V and ermeer, Y. Zhang, F. Waheed, M. Pham, and K. Szaszi. The epidermal growth factor receptor mediates tumor necrosis factor-Alpha-induced activation of the ERK/GEF-H1/RhoA pathway in tubular epithelium. Journal of Biological Chemistry 286 (2011), 9268-9279.
3. C. Emmanuel, M. Huynh, J. Matthews, E. Kelly, and H. Zoellner. TNF-Alpha and TGF-beta synergistically stimulate elongation of human endothelial cells without transdifferentiation to smooth muscle cell phenotype. Cytokine 61 (2013), 38-40.
4. W. Cao, S.L. Ma, J. Tang, J. Shi, and Y. Lu. A combined treatment TNF-Alpha/doxorubicin alleviates the resistance of MCF-7/Adr cells to cytotoxic treatment. Biochimica et Biophysica Acta 1763 (2006), 182-187.
5. A. Sacchi, A. Gasparri, C. Gallo-Stampino, S. Toma, F. Curnis, and A. Corti. Synergistic antitumor activity of cisplatin, paclitaxel, and gemcitabine with tumor vasculature-Targeted tumor necrosis factor-Alpha. Clinical Cancer Research 12 (2006), 175-182.
6. D.R. Spriggs, M.L. Sherman, H. Michie, K.A. Arthur, K. Imamura, D. Wilmore, E. Frei, and D.W. Kufe. Recombinant human tumor necrosis factor administered as a 24-hour intravenous infusion. A phase I and pharmacologic study, Journal of the National Cancer Institute 80 (1988), 1039-1044.
7. J. Li, R. Zheng, and Z. Wang. Mechanisms of the induction of apoptosis in human hepatoma cells by tumour necrosis factor-Alpha. Cell Biology International 25 (2001), 1213-1219.
8. D. Tang, H.J. Park, S.P. Georgescu, S.M. Sebti, A.D. Hamilton, and J.B. Galper. Simvastatin potentiates tumor necrosis factor alpha-mediated apoptosis of human vascular endothelial cells via the inhibition of the geranylgeranylation of RhoA. Life Sciences 79 (2006), 1484-1492.
9. K. Fukui, S. Tamura, A. Wada, Y. Kamada, Y. Sawai, K. Imanaka, N. Hayashi, I. Shimomura, and N. Hayashi. Expression and prognostic role of RhoAGTPases in hepatocellular carcinoma. Journal of Cancer Research and Clinical Oncology's 132 (2006), 627-633.
10. A.P. Struckhoff, M.K. Rana, and R.A. Worthylake. RhoA can lead the way in tumor cell invasion and metastasis. Frontiers in Bioscience 16 (2011), 1915-1926.
11. X.R. Li, F. Ji, J. Ouyang, W. Wu, L.Y. Qian, and K.Y. Yang. Overexpression of RhoA is associated with poor prognosis in hepatocellular carcinoma. European Journal of Surgical Oncology 32 (2006), 1130-1134.
12. X.L. Deng, F. He, L.F. Yang, L.W. Wu, and F. Yin. A novel mechanism of RhoA activation induced by tumor necrosis factor-α in mouse cerebral microvascular endothelial cells. Chinese Journal of Pathophysiology 27 (2011), 1210-1212.
13. S. Lim, J. Ryu, J.A. Shin, M.J. Shin, Y.K. Ahn, J.J. Kim, and K.H. Han. Tumor necrosis factor-Alpha potentiates RhoAmediated monocyte transmigratory activity in vivo at a picomolar level. Arteriosclerosis Thrombosis and Vascular Biology 29 (2009), 2138-2145.
14. T. Tuschl. Exp and ing small RNA interference. Nature Biotechnology 20 (2002), 446-448.
15. Q.Q. Liu, K. Yin, S. Zhu, L. Zhang, P.E. Wen, C.L. Li, D.B. Zhang, M. Liu, and G. Yan. Inhibition of C35 gene expression by small interfering RNA induces apoptosis of breast cancer cells. Bioscience Trends 4 (2010), 254-259.
16. G. Yan, R. Duan, K. Yin, S. Zhu, Q. Liu, M. Gong, H. Wang, C. Sun, D. Pu, N. Tang, and A.L. Huang. Inhibition of survivin expression to induce the apoptosis of hepatocarcinoma cells by adenovirus-mediated siRNA. Bioscience Trends 2 (2008), 88-93.
17. T.C. He, S. Zhou, L.T. da Costa, J. Yu, K.W. Kinzler, and B. Vogelstein. A simplified system for generating recombinant adenoviruses. Proceedings of the National Academy of Sciences of the United States of America 95 (1998), 2509-2514.
18. M. Sioud. RNA interference: Mechanisms, technical challenges, and therapeutic opportunities. Methods in Molecular Biology 1218 (2015), 1-15.
19. G. Li, H. Chang, Y.P. Zhai, and W. Xu. Targeted silencing of inhibitors of apoptosis proteins with siRNAs: A potential anti-cancer strategy for hepatocellular carcinoma. Asian Pacific Journal of Cancer Prevention 14 (2013), 4943-4952.
20. K. Yin, Q.Q. Liu, S. Zhu, and G. Yan. Adenovirus-mediated siRNA inhibited survivin gene expression induces tumor cell apoptosis in nude mice. Bioscience Trends 2 (2008), 231-234.
21. H. Wang, G. Zhao, X. Liu, A. Sui, K. Yang, R. Yao, Z. Wang, and Q. Shi. Silencing of RhoA and RhoC expression by RNA interference suppresses human colorectal carcinoma growth in vivo. Journal of Experimental & Clinical Cancer Research 29 (2010), 123.
22. C.C. Wong, C.M. Wong, S.L. Au, and I.O. Ng. RhoGTPases and Rho-effectors in hepatocellular carcinoma metastasis: ROCK N'Rhomove it. Liver International 30 (2010), 642-656.
23. C.M. Wong, J.W. Yam, Y.P. Ching, T.O. Yau, T.H. Leung, D.Y. Jin, and I.O. Ng. Rho GTPase-Activating protein deleted in liver cancer suppresses cell proliferation and invasion in hepatocellular carcinoma. Cancer Research 65 (2005), 8861-8868.
24. R. Rathinam, A. Berrier, and S.K. Alahari. Role of Rho GTPases and their regulators in cancer progression. Frontiers in Bioscience-L and mark 16 (2011), 2561-2571.
25. R.A. Cardone, A. Bellizzi, G. Busco, E.J. Weinman, M.E. Dell'Aquila, V. Casavola, A. Azzariti, A. Mangia, A. Paradiso, and S.J. Reshkin. The NHERF1 PDZ2 domain regulates PKA-RhoA-p38-mediated NHE1 activation and invasion in breast tumor cells. Molecular Biology of The Cell 18 (2007), 1768-1780.
26. F. Grise, A. Bidaud, and V. Moreau. Rho GTPases in hepatocellular carcinoma. Biochimica et Biophysica Acta-General Subjects 1795 (2009), 137-151.
27. L. Gou, W. Wang, A. Tong, Y. Yao, Y. Zhou, C. Yi, and J. Yang. Proteomic identification of RhoA as a potential biomarker for proliferation and metastasis in hepatocellular carcinoma. Journal of Molecular Medicine (Berlin). 89 (2011), 817-827.
28. A.Z. Hang, Q. Wang, Z. Han, W. Hu, L. Xi, Q. Gao, S. Wang, J. Zhou, G. Xu, L. Meng, G. Chen, and D. Ma. Reduced expression of Snail decreases breast cancer cell motility by down-regulating the expression and inhibiting the activity of RhoAGTPase. Oncology Letters 6 (2013), 339-346.
29. C.L. Williams. A new signaling paradigm to control the prenylation and trafficking of small GTPases. Cell Cycle 12 (2013), 2933-2934.
30. X. Wang, H. He, Y. Lu, W. Ren, K.Y. Teng, C.L. Chiang, Z. Yang, B. Yu, S. Hsu, S.T. Jacob, K. Ghoshal, and L.J. Lee. Indole-3-carbinol inhibits tumorigenicity of hepatocellular carcinoma cells via suppression of microRNA-21 and upregulation of phosphatase and tensin homolog. Biochimica et Biophysica Acta-general Subjects 1853 (2015), 244-253.
31. Z. Li, X. Dong, Z. Wang, W. Liu, N. Deng, Y. Ding, L. Tang, T. Hla, R. Zeng, L. Li, and D. Wu. Regulation of PTEN by Rho small GTPases. Nature Cell Biology 7 (2005), 399-404.
32. S. Yang, and H.M. Kim. The RhoA-ROCK-PTEN pathway as a molecular switch for anchorage dependent cell behavior. Biomaterials 33 (2012), 2902-2915.
33. F. Fusella, R. Ferretti, D. Recupero, S. Rocca, A. Di Savino, G. Tornillo, L. Silengo, E. Turco, S. Cabodi, P. Provero, P.P. P and olfi, A. Sapino, G. Tarone, and M. Brancaccio. Morgana acts as a proto-oncogene through inhibition of a ROCK-PTEN pathway. Journal of Patholoy and Bacteriology 234 (2014), 152-163.
34. K. Goto, Y. Chiba, K. Matsusue, Y. Hattori, Y. Maitani, H. Sakai, S. Kimura, and M. Misawa. The proximal STAT6 and NF-kappaB sites are responsible for IL-13- And TNF-Alpha-induced RhoA transcriptions in human bronchial smooth muscle cells. Pharmacological Research 61 (2010), 466-472.
35. K. Goto, Y. Chiba, H. Sakai, and M. Misawa. Tumor necrosis factor-Alpha (TNF-Alpha) induces up-regulation of RhoA via NF-kappaB activation in cultured human bronchial smooth muscle cells. Journal of Pharmacological Sciences 110 (2009), 437-444.
36. E. Kakiashvili, P. Speight, F. Waheed, R. Seth, M. Lodyga, S. Tanimura, M. Kohno, O.D. Rotstein, A. Kapus, and K. Szaszi. GEF-H1 mediates tumor necrosis factor-Alpha-induced Rho activation and myosin phosphorylation: Role in the regulation of tubular paracellular permeability. Journal of Biological Chemistry 284 (2009), 11454-11466.
37. F. Balkwill. TNF-Alpha in promotion and progression of cancer, cancer and Metastasis Reviews 25 (2006), 409-416.