Comparison of boiling and robotics automation method in DNA extraction for metagenomic sequencing of human oral microbes

PLoS ONE

Volumn 11, Issue 4, 2016, Pages

  Yamagishi, Junya ^a,b   Sato, Yukuto ^b   Shinozaki, Natsuko ^b   Ye, Bin ^b   Tsuboi, Akito ^b   Nagasaki, Masao ^b   Yamashita, Riu ^b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S; BACTERIAL DNA;

ADULT; ARTICLE; BACTERIAL FLORA; BOILING AUTOMATION METHOD; COMPLETENESS; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DNA EXTRACTION; DNA SEQUENCE; FEMALE; FIRMICUTES; FUSOBACTERIA; GENE SEQUENCE; HANDLING TIME; HEALTH STATUS; HUMAN; INTERMETHOD COMPARISON; MALE; METAGENOMIC SEQUENCING; MOUTH FLORA; NONHUMAN; POWERSOIL METHOD; PROCEDURES, PARAMETERS AND DEVICES; QIASYMPHONY AUTOMATION METHOD; RELIABILITY; REPRODUCIBILITY; TOOTH PLAQUE; ADSORPTION; COMPARATIVE STUDY; DEVICES; GENETICS; HEAT; HIGH THROUGHPUT SEQUENCING; ISOLATION AND PURIFICATION; LIQUID PHASE MICROEXTRACTION; METAGENOMICS; MICROBIOLOGY; MICROFLORA; PILOT STUDY; PRINCIPAL COMPONENT ANALYSIS; PROCEDURES; ROBOTICS;

ADSORPTION; DENTAL PLAQUE; DNA, BACTERIAL; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HOT TEMPERATURE; HUMANS; LIQUID PHASE MICROEXTRACTION; MALE; METAGENOMICS; MICROBIOTA; PILOT PROJECTS; PRINCIPAL COMPONENT ANALYSIS; RNA, RIBOSOMAL, 16S; ROBOTICS; SEQUENCE ANALYSIS, DNA;

EID: 84979026067     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0154389     Document Type: Article

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