Comparison of direct boiling method with commercial kits for extracting fecal microbiome DNA by Illumina sequencing of 16S rRNA tags

Journal of Microbiological Methods

Volumn 95, Issue 3, 2013, Pages 455-462

  Peng, Xin ^a   Yu, Ke Qiang ^a   Deng, Guan Hua ^a   Jiang, Yun Xia ^a   Wang, Yu ^a   Zhang, Guo Xia ^a   Zhou, Hong Wei ^a  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

Author keywords

DNA extraction; Feces; Illumina; Microbial diversity; Microbiome

Indexed keywords

RNA 16S;

ARTICLE; CLUSTER ANALYSIS; CONTROLLED STUDY; COST CONTROL; DIRECT BOILING METHOD; DNA EXTRACTION; FECES MICROFLORA; INTERMETHOD COMPARISON; METAGENOMICS; MICROBIOME; NONHUMAN; OPERATIONAL TAXONOMIC UNIT; PRINCIPAL COORDINATE ANALYSIS; PRIORITY JOURNAL; RNA SEQUENCE; SAMPLING; SPECIES DIVERSITY; TAXONOMY;

BACTERIA (MICROORGANISMS); OTUS;

DNA EXTRACTION; FECES; ILLUMINA; MICROBIAL DIVERSITY; MICROBIOME;

ANIMALS; COSTS AND COST ANALYSIS; DNA; FECES; HUMANS; MICROBIOTA; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SPECIMEN HANDLING; TIME FACTORS;

EID: 84889102325     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.07.015     Document Type: Article

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