Chelex-based DNA isolation procedure for the identification of microbial communities of eggshell surfaces

Analytical Biochemistry

Volumn 397, Issue 2, 2010, Pages 253-255

  Martín Platero, Antonio Manuel ^a   Peralta Sánchez, Juan Manuel ^b   Soler, Juan José ^b   Martínez Bueno, Manuel ^a  

^a UNIVERSITY OF GRANADA   (Spain)

^b DEPARTAMENTO DE ECOLOGÍA FUNCIONAL Y EVOLUTIVA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BIRDS; MICROORGANISMS;

AVIAN SPECIES; BACTERIAL DENSITY; DNA EXTRACTION; DNA ISOLATION; EGG VIABILITIES; MICROBIAL COMMUNITIES; RIBOSOMAL GENES; WILD BIRDS;

DNA;

DNA 16S;

ARTICLE; BACTERIAL COUNT; BACTERIUM EXAMINATION; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; COST BENEFIT ANALYSIS; DNA DETERMINATION; DNA EXTRACTION; DNA ISOLATION; EGG SHELL; FOWL; GEL ELECTROPHORESIS; GENE AMPLIFICATION; MICROBIAL COMMUNITY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

AVES; BACTERIA (MICROORGANISMS);

EID: 72049087994     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.10.041     Document Type: Article

References (13)

1. Bruce J., and Drysdale E.M. Trans-shell transmission. In: Board R.G., and Fuller R. (Eds). Microbiology of the Avian Egg (1994), Chapman & Hall, London 63-91
2. Rappé M.S., and Giovannoni S.J. The uncultured microbial majority. Annu. Rev. Microbiol. 57 (2003) 369-394
3. Schleifer K.H. Microbial diversity: facts, problems, and prospects. Syst. Appl. Microbiol. 27 (2004) 3-9
4. Shawkey M.D., Firestone M.K., Brodie E.L., and Beissinger S.R. Avian incubation inhibits growth and diversification of bacterial assemblages on eggs. PLoS ONE 4 (2009) e4522
5. Scheu P.M., Berghof K., and Stahl U. Detection of pathogenic and spoilage micro-organisms in food with the polymerase chain reaction. Food Microbiol. 15 (1998) 13-31
6. Huang C.W., and Huang M.C. A simple and rapid PCR-based method for ostrich sexing using micro amounts of DNA. Reprod. Domest. Anim. 43 (2008) 643-646
7. Tomasek O., Kubicek O., and Tukac V. Comparison of three template preparation methods for routine detection of beak and feather disease virus and avian polyomavirus with single and nested polymerase chain reaction in clinical specimens. Avian Pathol. 37 (2008) 145-149
8. de Lamballerie X., Zandotti C., Vignoli C., BolLlet C., and de Micco P. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Res. Microbiol. 143 (1992) 785-790
9. Giraffa G., Rossetti L., and Neviani E. An evaluation of chelex-based DNA purification protocols for the typing of lactic acid bacteria. J. Microbiol. Methods 42 (2000) 175-184
10. Ogier J.C., Son O., Gruss A., Tailliez P., and Delacroix-Buchet A. Identification of the bacterial microflora in dairy products by temporal temperature gradient gel electrophoresis. Appl. Environ. Microbiol. 68 (2002) 3691-3701
11. Cook M.I., Beissinger S.R., Toranzos G.A., Rodriguez R.A., and Arendt W.J. Trans-shell infection by pathogenic micro-organisms reduces the shelf life of non-incubated bird's eggs: a constraint on the onset of incubation?. Proc. R. Soc. Lond. B 270 (2003) 2233-2240
12. Ishii S., and Loynachan T.E. Rapid and reliable DNA extraction techniques from trypan-blue-stained mycorrhizal roots: comparison of two methods. Mycorrhiza 14 (2004) 271-275
13. Radström P., Knutsson R., Wolffs P., Lövenklev M., and Löfström C. Pre-PCR processing. Mol. Biotechnol. 26 (2004) 133-146