Characterization of a new Baeyer-Villiger monooxygenase and conversion to a solely N-or S-oxidizing enzyme by a single R292 mutation

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1864, Issue 9, 2016, Pages 1177-1187

  Catucci, Gianluca ^a   Zgrablic, Ivan ^a   Lanciani, Francesco ^a   Valetti, Francesca ^a   Minerdi, Daniela ^a   Ballou, David P ^b   Gilardi, Gianfranco ^a   Sadeghi, Sheila J ^a  

^a UNIVERSITY OF TURIN   (Italy)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

BVMO; Drug metabolites; Ethionamide; Flavoprotein; Isothermal calorimetry; Stopped flow

Indexed keywords

ALANINE; ARGININE; ETHIONAMIDE; GLYCINE; KETONE; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; RIBOFLAVIN; TOZASERTIB; UNSPECIFIC MONOOXYGENASE; BACTERIAL PROTEIN; C4A-HYDROPEROXYFLAVIN; MIXED FUNCTION OXIDASE; RECOMBINANT PROTEIN; RIBOFLAVIN DERIVATIVE;

ARTICLE; BAEYER VILLIGER REACTION; CATALYSIS; ENZYME ACTIVITY; GAS CHROMATOGRAPHY; GENE MUTATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTHERMAL TITRATION CALORIMETRY; KINETICS; MOLECULAR CLONING; OXYGENATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; SPECTROSCOPY; STEADY STATE; THERMOBIFIDA FUSCA; ACINETOBACTER; AMINO ACID SEQUENCE; BIOCATALYSIS; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; MICROBIOLOGY; MUTATION; OXIDATION REDUCTION REACTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

ACINETOBACTER; ALANINE; AMINO ACID SEQUENCE; ARGININE; BACTERIAL PROTEINS; BIOCATALYSIS; CATALYTIC DOMAIN; CLONING, MOLECULAR; ESCHERICHIA COLI; ETHIONAMIDE; FLAVINS; GENE EXPRESSION; GLYCINE; KETONES; KINETICS; MIXED FUNCTION OXYGENASES; MUTAGENESIS, SITE-DIRECTED; MUTATION; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SOIL MICROBIOLOGY; SUBSTRATE SPECIFICITY;

EID: 84976892499     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2016.06.010     Document Type: Article

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