A Novel NADPH-dependent flavoprotein reductase from Bacillus megaterium acts as an efficient cytochrome P450 reductase

Journal of Biotechnology

Volumn 231, Issue , 2016, Pages 83-94

  Milhim, Mohammed ^a   Gerber, Adrian ^a   Neunzig, Jens ^a   Hannemann, Frank ^a   Bernhardt, Rita ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

Bacillus megaterium; CYP106A1; CYP21A2; Diflavin reductase; Microsomal P450; NADPH dependent cytochrome P450 oxidoreductase

Indexed keywords

BACTERIOLOGY; BIOTECHNOLOGY; ELECTRON TRANSITIONS; ESCHERICHIA COLI; PROTEINS; PURIFICATION;

BACILLUS MEGATERIUM; CYP106A1; CYP21A2; DIFLAVIN REDUCTASE; MICROSOMAL P450; OXIDOREDUCTASES;

ENZYME ACTIVITY;

4 HYDROXYTESTOSTERONE; CYTOCHROME C; CYTOCHROME P450 REDUCTASE; DEOXYCORTICOSTERONE; FLAVINE ADENINE NUCLEOTIDE; FLAVINE MONONUCLEOTIDE; PROGESTERONE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FLAVOPROTEIN REDUCTASE; TESTOSTERONE; UNCLASSIFIED DRUG; CYTOCHROME P450; FLAVOPROTEIN; OXIDOREDUCTASE; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS MEGATERIUM; BIOCATALYST; BIOTRANSFORMATION; ELECTRON TRANSPORT; ESCHERICHIA COLI; GENE EXPRESSION; HETEROLOGOUS EXPRESSION; HYDROXYLATION; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; THERMOSTABILITY; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM;

BACILLUS MEGATERIUM; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME STABILITY; ESCHERICHIA COLI; FLAVOPROTEINS; OXIDOREDUCTASES; RECOMBINANT PROTEINS;

EID: 84976544832     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2016.05.035     Document Type: Article

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