Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood

Biotechnology for Biofuels

Volumn 9, Issue 1, 2016, Pages

  Herring, Christopher D ^a,b   Kenealy, William R ^a,c   Joe Shaw, A ^a,d   Covalla, Sean F ^a   Olson, Daniel G ^b,e   Zhang, Jiayi ^a,f   Ryan Sillers, W ^a,g   Tsakraklides, Vasiliki ^a,d   Bardsley, John S ^a   Rogers, Stephen R ^a   Thorne, Philip G ^a   Johnson, Jessica P ^a,h   Foster, Abigail ^a   Shikhare, Indraneel D ^a,i   Klingeman, Dawn M ^e,j   Brown, Steven D ^e,j   Davison, Brian H ^e,j   Lynd, Lee R ^a,b,e   Hogsett, David A ^a,k  

^a Mascoma Corporation   (United States)

^b DARTMOUTH COLLEGE   (United States)

^c Verdezyne   (United States)

^d Novogy Inc   (United States)

^e BioEnergy Science Center   (United States)

^f GENZYME CORPORATION   (United States)

^g Myriant Corporation   (United States)

^h Nalco Champion ^*  (United States)

ⁱ Nalco Champion   (United States)

^j OAK RIDGE NATIONAL LABORATORY   (United States)

^k Novozymes Biotech Inc   (United States)

more..

Author keywords

Bioprocess development; Cellulosic ethanol; Consolidated bioprocessing; Metabolic engineering; Organism development; Thermophilic bacteria

Indexed keywords

BACTERIA; BATCH CELL CULTURE; BIOLOGY; CELLULOSE; CELLULOSIC ETHANOL; FERMENTATION; GENES; HARDWOODS; METABOLIC ENGINEERING; SACCHARIFICATION;

BIOPROCESS DEVELOPMENT; BIOPROCESS IMPROVEMENTS; CONSOLIDATED BIO-PROCESSING; INDUSTRIAL PRODUCTION; ORGANISM DEVELOPMENT; PHOSPHOTRANSACETYLASE; SIMULTANEOUS SACCHARIFICATION AND FERMENTATION; THERMOPHILIC BACTERIA;

ETHANOL;

BIOTECHNOLOGY; ETHANOL; FERMENTATION; METABOLISM; THERMOPHILIC BACTERIUM; WOOD; YEAST;

BACTERIA (MICROORGANISMS); THERMOANAEROBACTERIUM SACCHAROLYTICUM;

EID: 84975849537     PISSN: 17546834     EISSN: None     Source Type: Journal    
DOI: 10.1186/s13068-016-0536-8     Document Type: Article

References (25)

1. Zacchi G, Axelsson A. Economic evaluation of preconcentration in production of ethanol from dilute sugar solutions. Biotechnol Bioeng. 1989;34:223-33.
2. Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev. 2002;66:506-77.
3. Humbird D, Davis R, Tao L, Kinchin C, Hsu D, Aden A, Schoen P, Lukas J, Olthof B, Worley M, Sexton D, Dudgeon D. Process design and economics for conversion of lignocellulosic biomass to ethanol. NREL technical report NREL/TP-5100-51400. 2011.
4. Laser M, Jin H, Jayawardhana K, Dale BE, Lynd LR. Projected mature technology scenarios for conversion of cellulosic biomass to ethanol with coproduction thermochemical fuels, power, and/or animal feed protein. Biofuels Bioprod Biorefin. 2009;3:231-46.
5. Paye J, Guseva A, Hammer S, Gjersing E, Davis M, Davison B, Olstad J, Donohoe B, Nguyen T, Wyman C, Pattathil S, Hahn M, Lynd L. Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment. Biotechnol Biofuels. 2016;9:8.
6. Shaw AJ, Podkaminer KK, Desai SG, Bardsley JS, Rogers SR, Thorne PG, Hogsett D, Lynd LR. Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield. Proc Natl Acad Sci USA. 2008;105:13769-74.
7. Shaw AJ, Hogsett DA, Lynd LR. Natural competence in thermoanaerobacter and thermoanaerobacterium species. Appl Environ Microbiol. 2010;76:4713-9.
8. Currie DH, Raman B, Gowen CM, Tschaplinski TJ, Land ML, Brown SD, Covalla SF, Klingeman DM, Yang ZK, Engle NL, Johnson CM, Rodriguez M, Shaw a J, Kenealy WR, Lynd LR, Fong SS, Mielenz JR, Davison BH, Hogsett D, Herring CD. Genome-scale resources for Thermoanaerobacterium saccharolyticum. BMC Syst Biol. 2015;9:30.
9. Shaw AJ, Covalla SF, Miller BB, Firliet BT, Hogsett DA, Herring CD. Urease expression in a Thermoanaerobacterium saccharolyticum ethanologen allows high titer ethanol production. Metab Eng. 2012;14:528-32.
10. Herring C, Kenealy W, Shaw A, Raman B, Tschaplinski T, Brown S, Davison B, Covalla S, Sillers W, Xu H, Tsakraklides V, Hogsett D. Final report on development of Thermoanaerobacterium saccharolyticum for the conversion of lignocellulose to ethanol. US Dep Energy Tech Rep DOE/GO/017057-1 2012.
11. Podkaminer KK, Kenealy WR, Herring CD, Hogsett DA, Lynd LR. Ethanol and anaerobic conditions reversibly inhibit commercial cellulase activity in thermophilic simultaneous saccharification and fermentation (tSSF). Biotechnol Biofuels. 2012;5:43.
12. Currie DH, Herring CD, Guss AM, Olson DG, Hogsett DA, Lynd LR. Functional heterologous expression of an engineered full length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolyticum. Biotechnol Biofuels. 2013;6:32.
13. Shaw AJ, Covalla SF, Hogsett DA, Herring CD. Marker removal system for Thermoanaerobacterium saccharolyticum and development of a markerless ethanologen. Appl Environ Microbiol. 2011;77:2534-6.
14. Kacmar J, Gilbert A, Cockrell J, Srienc F. The cytostat: a new way to study cell physiology in a precisely defined environment. J Biotechnol. 2006;126:163-72.
15. Borden JR, Papoutsakis ET. Dynamics of genomic-library enrichment and identification of solvent tolerance genes for Clostridium acetobutylicum. Appl Environ Microbiol. 2007;73:3061-8.
16. Shaw AJ, Miller BB, Rogers SR, Kenealy WR, Meola A, Bhandiwad A, Sillers WR, Shikhare I, Hogsett DA, Herring CD. Anaerobic detoxification of acetic acid in a thermophilic ethanologen. Biotechnol Biofuels. 2015;8:75.
17. Hillmann F, Fischer R-J, Saint-Prix F, Girbal L, Bahl H. PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum. Mol Microbiol. 2008;68:848-60.
18. Russell JB. Glucose toxicity in Prevotella ruminicola: methylglyoxal accumulation and its effect on membrane physiology. Appl Environ Microbiol. 1993;59:2844-50.
19. Zhou J, Olson DG, Lanahan AA, Tian L, Murphy SJL, Lo J, Lynd LR. Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485. Biotechnol Biofuels. 2015;8:138.
20. Currie DH, Guss AM, Herring CD, Giannone RJ, Johnson CM, Lankford PK, Brown SD, Hettich RL, Lynd LR. Profile of secreted hydrolases, associated proteins, and SlpA in Thermoanaerobacterium saccharolyticum during the degradation of hemicellulose. Appl Environ Microbiol. 2014;80:5001-11.
21. Olson DG, McBride JE, Shaw AJ, Lynd LR. Recent progress in consolidated bioprocessing. Curr Opin Biotechnol. 2012;23(3):396-405.
22. Lynd LR, Guss AM, Beri D, Herring C, Holwerda EK, Murphy SJ, Olson DG, Paye J, Rydzak T, Shao X, Tian L, Worthen R. Advances in consolidated bioprocessing using Clostridium thermocellum and Thermoanaerobacterium saccharolyticum. In: Liao J, Wittmann C, editors. Industrial biotechnology. Weinheim: Wiley-VCH; 2016.
23. Cray JA, Stevenson A, Ball P, Bankar SB, Eleutherio ECA, Ezeji TC, Singhal RS, Thevelein JM, Timson DJ, Hallsworth JE. Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms. Curr Opin Biotechnol. 2015;49:228-59.
24. Shanks RMQ, Caiazza NC, Hinsa SM, Toutain CM, O'Toole GA. Saccharomyces cerevisiae-based molecular tool kit for manipulation of genes from gram-negative bacteria. Appl Environ Microbiol. 2006;72:5027-36.
25. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;13:403-10.