Radiolabeling of Extracellular Vesicles with 99mTc for Quantitative in Vivo Imaging Studies

Cancer Biotherapy and Radiopharmaceuticals

Volumn 31, Issue 5, 2016, Pages 168-173

  Varga, Zoltán ^a   Gyurkó, István ^a   Pálóczi, Krisztina ^b   Buzás, Edit I ^b   Horváth, Ildikó ^b   Hegedus, Nikolett ^b   Máthé, Domokos ^b,c   Szigeti, Krisztián ^b  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b SEMMELWEIS UNIVERSITY   (Hungary)

^c CROmed Ltd   (Hungary)

Author keywords

biodistribution; exosome; microvesicle; SPECT

Indexed keywords

CYSTEINE; HISTIDINE; LIPOSOME; METHIONINE; TECHNETIUM 99M; RADIOPHARMACEUTICAL AGENT; TECHNETIUM COMPLEX;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; COMPUTER ASSISTED TOMOGRAPHY; CONTROLLED STUDY; ERYTHROCYTE; EXOSOME; ISOTOPE LABELING; LIVER; MALE; MOUSE; NONHUMAN; PHOSPHOLIPID BILAYER; PRIORITY JOURNAL; RADIOACTIVITY; SINGLE PHOTON EMISSION COMPUTER TOMOGRAPHY; SPLEEN; TRANSMISSION ELECTRON MICROSCOPY; ANIMAL; BAGG ALBINO MOUSE; CHEMISTRY; HUMAN; METABOLISM; PROCEDURES; SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY-COMPUTED TOMOGRAPHY; TISSUE DISTRIBUTION; ULTRASTRUCTURE;

ANIMALS; ERYTHROCYTES; EXOSOMES; EXTRACELLULAR VESICLES; HUMANS; ISOTOPE LABELING; MALE; MICE; MICE, INBRED BALB C; ORGANOTECHNETIUM COMPOUNDS; RADIOPHARMACEUTICALS; SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY COMPUTED TOMOGRAPHY; TISSUE DISTRIBUTION;

EID: 84975477717     PISSN: 10849785     EISSN: 15578852     Source Type: Journal    
DOI: 10.1089/cbr.2016.2009     Document Type: Article

References (38)

1. Lee TH, D'Asti E, Magnus N, et al. Microvesicles as mediators of intercellular communication in cancer-The emerging science of cellular "debris." Semin Immunopathol 2011;33:455.
2. Simons M, Raposo G. Exosomes-Vesicular carriers for intercellular communication. Curr Opin Cell Biol 2009;21:575.
3. Mathivanan S, Ji H, Simpson RJ. Exosomes: Extracellular organelles important in intercellular communication. J Proteomics 2010;73:1907.
4. Yuana Y, Sturk A, Nieuwland R. Extracellular vesicles in physiological and pathological conditions. Blood Rev 2012; 27:31.
5. van der Pol E, Böing AN, Harrison P, et al. Classification, functions, and clinical relevance of extracellular vesicles. Pharmacol Rev 2012;64:676.
6. György B, Szabó TG, Pásztói M, et al. Membrane vesicles, current state-of-the-art: Emerging role of extracellular vesicles. Cell Mol Life Sci 2011;68:2667.
7. Biller SJ, Schubotz F, Roggensack SE, et al. Bacterial vesicles in marine ecosystems. Science 2014;343:183.
8. Abstracts from the Third International Meeting of ISEV 2014 Rotterdam, The Netherlands, April 30th-May 3rd, 2014. J Extracell Vesicles 2014;3:24214.
9. Andaloussi SE, Mäger I, Breakefield XO, et al. Extracellular vesicles: Biology and emerging therapeutic opportunities. Nat Rev Drug Discov 2013;12:347.
10. Lener T, Gioma M, Aigner L, et al. Applying extracellular vesicles based therapeutics in clinical trials-an ISEV position paper. J Extracell Vesicles 2015;4:30087.
11. Zomer A, Maynard C, Verweij FJ, et al. In vivo imaging reveals extracellular vesicle-mediated phenocopying of metastatic behavior. Cell 2015;161:1046.
12. Sadovska L, Santos CB, Kalninxa Z, et al. Biodistribution, uptake and effects caused by cancer-derived extracellular vesicles. J Circ Biomarkers 2015;4:2.
13. Wiklander OP, Nordin JZ, O'Loughlin A, et al. Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting. J Extracell Vesicles 2015;4:26316.
14. Takahashi Y, Nishikawa M, Shinotsuka H, et al. Visualization and in vivo tracking of the exosomes of murine melanoma B16-BL6 cells in mice after intravenous injection. J Biotechnol 2013;165:77.
15. Lai CP, Mardini O, Ericsson M, et al. Dynamic biodistribution of extracellular vesicles in vivo using a multimodal imaging reporter. ACS Nano 2014;8:483.
16. Jang SC, Kim SR, Yoon YJ, et al. In vivo kinetic biodistribution of nano-sized outer membrane vesicles derived from bacteria. Small 2015;11:456.
17. Grange C, Tapparo M, Bruno S, et al. Biodistribution of mesenchymal stem cell-derived extracellular vesicles in a model of acute kidney injury monitored by optical imaging. Int J Mol Med 2014;33:1055.
18. Ohno S, Takanashi, M, Sudo K, et al. Systemically injected exosomes targeted to EGFR deliver antitumor microRNA to breast cancer cells. Mol Ther 2013;21:185.
19. Suetsugu A, Honma K, Saji S, et al. Imaging exosome transfer from breast cancer cells to stroma at metastatic sites in orthotopic nude-mouse models. Adv Drug Deliv Rev 2013;65:383.
20. Hirsjärvi S, Sancey L, Dufort S, et al. Effect of particle size on the biodistribution of lipid nanocapsules: Comparison between nuclear and fluorescence imaging and counting. Int J Pharm 2013;453:594.
21. Böing AN, Van Der Pol E, Grootemaat AE, et al. Singlestep isolation of extracellular vesicles by size-exclusion chromatography. J Extracell Vesicles 2014;3:23430.
22. Phillips WT, Goins BA, Bao A. Radioactive liposomes. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2009;1:69.
23. McAfee JG, Thakur, ML. Survey of radioactive agents for in vitro labeling of phagocytic leukocytes. I. Soluble agents. J Nucl Med Off Publ Soc Nucl Med 1976;17:480.
24. Goins B, Klipper R, Rudolph AS, et al. Biodistribution and imaging studies of technetium-99m-labeled liposomes in rats with focal infection. J Nucl Med Off Publ Soc Nucl Med 1993;34;2160.
25. Awasthi V, Goins B, McManus L, et al. [99m Tc] liposomes for localizing experimental colitis in arabbit model. Nucl Med Biol 2003;30:159.
26. Lee C-M, Choi Y, Huh EJ, et al. Polyethylene glycol (PEG) modified 99mTc-HMPAOliposome for improving blood circulation and biodistribution: The effect of the extent of PEGylation. Cancer Biother Radiopharm 2005; 20:620.
27. Dagar S, Krishnadas A, Rubinstein I, et al. VIP grafted sterically stabilized liposomes for targeted imaging of breast cancer: In vivo studies. J Control Release 2003; 91:123.
28. Erdogan S, Ozer AY, Ercan MT, et al. Scintigraphic imaging of infections with 99m-Tc-labelled glutathione liposomes. J Microencapsul 2000;17:459.
29. Oyen WJ, Boerman OC, Storm G, et al. Detecting infection and inflammation with technetium-99m-labeled Stealth (R) liposomes. J Nucl Med 1996;37:1392.
30. Tilcock C, Yap M, Szucs M, et al. PEG-coated lipid vesicles with encapsulated technetium-99m as blood pool agents for nuclear medicine. Nucl Med Biol 1994; 21:165.
31. Sou K, Goins B, Takeoka S, et al. Selective uptake of surface-modified phospholipid vesicles by bone marrow macrophages in vivo. Biomaterials 2007;28:2655.
32. Kleiter MM, Yu D, Mohammadian LA, et al. A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation. Clin Cancer Res 2006;12:6800.
33. Helbok A, Decristoforo C, Dobrozemsky G, et al. Radiolabeling of lipid-based nanoparticles for diagnostics and therapeutic applications: A comparison using different radiometals. J Liposome Res 2010;20:219.
34. Hwang do W, Choi H, Jang SC, et al. Noninvasive imaging of radiolabeled exosome-mimetic nanovesicle using (99m) Tc-HMPAO. Sci Rep 2015;5:15636.
35. Alberto R, Schibli R, Egli A, et al. A novel organometallic aqua complex of technetium for the labeling of biomolecules: Synthesis of [99mTc OH2) 3 (CO) 3]+ from [99mTcO4]- in aqueous solution and its reaction with a bifunctional ligand. J Am Chem Soc 1998;120:7987.
36. Schibli R, Schubiger AP. Current use and future potential of organometallic radiopharmaceuticals. Eur J Nucl Med Mol Imaging 2002;29:1529.
37. Egli A, Alberto R, Tannahill L, et al. Organometallic 99mTc-Aquaion labels peptide to an unprecedented high specific activity. J Nucl Med 1999;40:1913.
38. Veres DS, Máthé D, Futó I, et al. Quantitative liver lesion volume determination by nanoparticle-based SPECT. Mol Imaging Biol 2014;16:167.