CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes

Nature Biotechnology

Volumn 34, Issue 6, 2016, Pages 631-633

  Evers, Bastiaan ^a   Jastrzebski, Katarzyna ^a   Heijmans, Jeroen P M ^a   Grernrum, Wipawadee ^a   Beijersbergen, Roderick L ^a   Bernards, Rene ^a  

^a NETHERLANDS CANCER INSTITUTE   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL PROCESS; ESSENTIAL GENE; GENETIC SCREENS; HIGH THROUGHPUT; LOW NOISE; PALINDROMIC; SHORT HAIRPIN RNA (SHRNA);

BIOTECHNOLOGY;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT PROTEIN 9; CRISPR ASSOCIATED PROTEIN; GENOMIC DNA; RNA POLYMERASE; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG; GENETIC MARKER; SMALL INTERFERING RNA;

ARTICLE; CANCER CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT INTERFERENCE; COMPARATIVE STUDY; CONTROLLED STUDY; CRISPR CAS SYSTEM; ESSENTIAL GENE; GENE FUNCTION; GENE IDENTIFICATION; GENE TARGETING; GENETIC SCREENING; MOUSE; NEXT GENERATION SEQUENCING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PRODUCTIVITY; RNA INTERFERENCE; TRANSCRIPTION INITIATION SITE; EVALUATION STUDY; GENE EXPRESSION PROFILING; GENE INACTIVATION; GENETIC MARKER; GENETICS; PROCEDURES;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; GENE EXPRESSION PROFILING; GENE KNOCKOUT TECHNIQUES; GENETIC MARKERS; GENETIC TESTING; RNA, SMALL INTERFERING;

EID: 84974622979     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3536     Document Type: Article

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