Licochalcone A induces T24 bladder cancer cell apoptosis by increasing intracellular calcium levels

Molecular Medicine Reports

Volumn 14, Issue 1, 2016, Pages 911-919

  Yang, Xinhui ^a   Jiang, Jiangtao ^a   Yang, Xinyan ^a   Han, Jichun ^a   Zheng, Qiusheng ^a  

^a SHIHEZI UNIVERSITY   (China)

Author keywords

Apoptosis; Intracellular Ca2+; Licochalcone A; Reactive oxygen species; T24 cells

Indexed keywords

APOPTOTIC PROTEASE ACTIVATING FACTOR 1; BIM PROTEIN; CALCIUM; CALPAIN 2; CASPASE 3; CASPASE 4; CASPASE 9; FLUO 3; LICOCHALCONE A; PROTEIN BAX; PROTEIN BCL XL; REACTIVE OXYGEN METABOLITE; ANTINEOPLASTIC AGENT; APOPTOSIS REGULATORY PROTEIN; CHALCONE DERIVATIVE;

APOPTOSIS; ARTICLE; BLADDER CANCER; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CANCER CELL; CELL DEATH; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; ENDOPLASMIC RETICULUM STRESS; ENZYME ACTIVATION; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; HUMAN; HUMAN CELL; IC50; IN VITRO STUDY; MITOCHONDRIAL MEMBRANE POTENTIAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION; BLADDER TUMOR; CELL SURVIVAL; DOSE RESPONSE; DRUG EFFECTS; GENETICS; INTRACELLULAR SPACE; METABOLISM; MITOCHONDRION; TUMOR CELL LINE;

ANTINEOPLASTIC AGENTS, PHYTOGENIC; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CALCIUM; CASPASE 9; CELL LINE, TUMOR; CELL SURVIVAL; CHALCONES; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOPLASMIC RETICULUM STRESS; HUMANS; INTRACELLULAR SPACE; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; REACTIVE OXYGEN SPECIES; URINARY BLADDER NEOPLASMS;

EID: 84973495070     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2016.5334     Document Type: Article

References (36)

1. Wang X, Feng Y, Wang N, Cheung F, Tan HY, Zhong S, Li C and Kobayashi S: Chinese medicines induce cell death: The molecular and cellular mechanisms for cancer therapy. Biomed Res Int 2014: 530342, 2014.
2. Lu C and Mei X: Study on anti-tumor activity of extracts from cultured cells of Taxus chinensis. Zhong Yao Cai 26: 335-337, 2003 (In Chinese).
3. Wang Q, Du H, Geng G, Zhou H, Xu M, Cao H, Zhang B, Song G and Hu T: Matrine inhibits proliferation and induces apoptosis via BID-mediated mitochondrial pathway in esophageal cancer cells. Mol Biol Rep 41: 3009-3020, 2014.
4. Zhao H, Jiang JT and Zheng QS: Advance in studies on pharmacological effects of licochalcone A. Zhongguo Zhong Yao Za Zhi 38: 3814-3818, 2013 (In Chinese).
5. Fukai T, Marumo A, Kaitou K, Kanda T, Terada S and Nomura T: Anti-Helicobacter pylori fl avonoids from licorice extract. Life Sci 71: 1449-1463, 2002.
6. Funakoshi-Tago M, Nakamura K, Tsuruya K, Hatanaka M, Mashino T, Sonoda Y and Kasahara T: The fi xed structure of Licochalcone A by alpha, beta-unsaturated ketone is necessary for anti-infl ammatory activity through the inhibition of NF-kappaB activation. Int Immunopharmacol 10: 562-571, 2010.
7. Xiao XY, Hao M, Yang XY, Ba Q, Li M, Ni SJ, Wang LS and Du X: Licochalcone A inhibits growth of gastric cancer cells by arresting cell cycle progression and inducing apoptosis. Cancer Lett 302: 69-75, 2011.
8. Zheng Q and Ye M: Chemical analysis of Chinese herbal medicine Gan-Cao (licorice). J Chromatogr A 1216: 1954-1969, 2009.
9. Feldman M and Grenier D: Cranberry proanthocyanidins act in synergy with licochalcone A to reduce Porphyromonas gingivalis growth and virulence properties, and to suppress cytokine secretion by macrophages. J Appl Microbiol 113: 438-447, 2012.
10. Messier C and Grenier D: Effect of licorice compounds licochalcone A, glabridin and glycyrrhizic acid on growth and virulence properties of Candida albicans. Mycoses 54: e801-e806, 2011.
11. Furusawa J, Funakoshi-Tago M, Mashino T, Tago K, Inoue H, Sonoda Y and Kasahara T: Glycyrrhiza inflata-derived chalcones, Licochalcone A, Licochalcone B and Licochalcone D, inhibit phosphorylation of NF-kappaB p65 in LPS signaling pathway. Int Immunopharmacol 9: 499-507, 2009.
12. Lee CS, Kwak SW, Kim YJ, Lee SA, Park ES, Myung SC, Kim W, Lee MS and Lee JJ: Guanylate cyclase activator YC-1 potentiates apoptotic effect of licochalcone A on human epithelial ovarian carcinoma cells via activation of death receptor and mitochondrial pathways. Eur J Pharmacol 683: 54-62, 2012.
13. Kerr JF, Wyllie AH and Currie AR: Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26: 239-257, 1972.
14. Zamzami N, Marchetti P, Castedo M, Decaudin D, Macho A, Hirsch T, Susin SA, Petit PX, Mignotte B and Kroemer G: Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell death. J Exp Med 182: 367-377, 1995.
15. Kim J, Choi TG, Ding Y, Kim Y, Ha KS, Lee KH, Kang I, Ha J, Kaufman RJ, Lee J, et al: Overexpressed cyclophilin B suppresses apoptosis associated with ROS and Ca2+ homeostasis after ER stress. J Cell Sci 121: 3636-3648, 2008.
16. Santos CX, Tanaka LY, Wosniak J and Laurindo FR: Mechanisms and implications of reactive oxygen species generation during the unfolded protein response: Roles of endoplasmic reticulum oxidoreductases, mitochondrial electron transport, and NADPH oxidase. Antioxid Redox Signal 11: 2409-2427, 2009.
17. Yan Y, Wei CL, Zhang WR, Cheng HP and Liu J: Cross-talk between calcium and reactive oxygen species signaling. Acta Pharmacol Sin 27: 821-826, 2006.
18. Berridge MJ, Lipp P and Bootman MD: The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol 1: 11-21, 2000.
19. Nakagawa T and Yuan J: Cross-talk between two cysteine protease families. Activation of caspase-12 by calpain in apoptosis. J Cell Biol 150: 887-894, 2000.
20. Martinez JA, Zhang Z, Svetlov SI, Hayes RL, Wang KK and Larner SF: Calpain and caspase processing of caspase-12 contribute to the ER stress-induced cell death pathway in differentiated PC12 cells. Apoptosis 15: 1480-1493, 2010.
21. Tan Y, Dourdin N, Wu C, De Veyra T, Elce JS and Greer PA: Ubiquitous calpains promote caspase-12 and JNK activation during endoplasmic reticulum stress-induced apoptosis. J Biol Chem 281: 16016-16024, 2006.
22. Choi AY, Choi JH, Hwang KY, Jeong YJ, Choe W, Yoon KS, Ha J, Kim SS, Youn JH, Yeo EJ and Kang I: Licochalcone A induces apoptosis through endoplasmic reticulum stress via a phospholipase C?1-, Ca(2+)-, and reactive oxygen species-dependent pathway in HepG2 human hepatocellular carcinoma cells. Apoptosis 19: 682-697, 2014.
23. Yuan X, Li D, Zhao H, Jiang J, Wang P, Ma X, Sun X and Zheng Q: Licochalcone A-induced human bladder cancer T24 cells apoptosis triggered by mitochondria dysfunction and endoplasmic reticulum stress. Biomed Res Int 2013: 474272, 2013.
24. Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S and Boyd MR: New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst 82: 1107-1112, 1990.
25. Hockenbery D, Nuñez G, Milliman C, Schreiber RD and Korsmeyer SJ: Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-346, 1990.
26. Jung JI, Lim SS, Choi HJ, Cho HJ, Shin HK, Kim EJ, Chung WY, Park KK and Park JH: Isoliquiritigenin induces apoptosis by depolarizing mitochondrial membranes in prostate cancer cells. J Nutr Biochem 17: 689-696, 2006.
27. Vanden Hoek TL, Li C, Shao Z, Schumacker PT and Becker LB: Significant levels of oxidants are generated by isolated cardiomyocytes during ischemia prior to reperfusion. J Mol Cell Cardiol 29: 2571-2583, 1997.
28. Reers M, Smith TW and Chen LB: J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. Biochemistry 30: 4480-4486, 1991.
29. Carthagena L, Bergamaschi A, Luna JM, David A, Uchil PD, Margottin-Goguet F, Mothes W, Hazan U, Transy C, Pancino G and Nisole S: Human TRIM gene expression in response to interferons. PLoS One 4: e4894, 2009.
30. Rafi MM, Vastano BC, Zhu N, Ho CT, Ghai G, Rosen RT, Gallo MA and DiPaola RS: Novel polyphenol molecule isolated from licorice root (Glycyrrhiza glabra) induces apoptosis, G2/M cell cycle arrest, and Bcl-2 phosphorylation in tumor cell lines. J Agric Food Chem 50: 677-684, 2002.
31. Jiang J, Yuan X, Zhao H, Yan X, Sun X and Zheng Q: Licochalcone A inhibiting proliferation of bladder cancer T24 cells by inducing reactive oxygen species production. Biomed Mater Eng 24: 1019-1025, 2014.
32. Kaiser N and Edelman IS: Calcium dependence of glucocorticoid-induced lymphocytolysis. Proc Natl Acad Sci USA 74: 638-642, 1977.
33. McConkey DJ and Orrenius S: The role of calcium in the regulation of apoptosis. Biochem Biophys Res Commun 239: 357-366, 1997.
34. Sergeev IN: Calcium as a mediator of 1,25-dihydroxyvitamin D3-induced apoptosis. J Steroid Biochem Mol Biol 89-90: 419-425, 2004.
35. Mekahli D, Bultynck G, Parys JB, De Smedt H and Missiaen L: Endoplasmic-reticulum calcium depletion and disease. Cold Spring Harb Perspect Biol 3: a004317, 2011.
36. Matsuzaki S, Hiratsuka T, Kuwahara R, Katayama T and Tohyama M. Caspase-4 is partially cleaved by calpain via the impairment of Ca2+ homeostasis under the ER stress. Neurochem Int 56: 352-356, 2010.