Single chain antibodies as tools to study transforming growth factor-β-regulated SMAD proteins in proximity ligation-based pharmacological screens

Molecular and Cellular Proteomics

Volumn 15, Issue 6, 2016, Pages 1848-1856

  Blokzijl, Andries ^a,d   Zieba, Agata ^a   Hust, Michael ^a   Schirrmann, Thomas ^c,d   Helmsing, Saskia ^c   Grannas, Karin ^a   Hertz, Ellen ^a   Moren, Anita ^a   Chen, Lei ^a   Söderberg, Ola ^a   Moustakas, Aristidis ^b   Dübel, Stefan ^c   Landegren, Ulf ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b UPPSALA UNIVERSITY   (Sweden)

^c TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^d YUMAB GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTALLIZABLE ANTIBODY FRAGMENT; CYCLIN DEPENDENT KINASE 4; IMMUNOGLOBULIN G; INSULIN RECEPTOR INHIBITOR; PHOSPHOTRANSFERASE INHIBITOR; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; SMAD PROTEIN; SMAD2 PROTEIN; SMAD3 PHOSPHORYLATED IN THE LINKER DOMAIN 3; SMAD3 PROTEIN; SMAD4 PROTEIN; SMAD7 PROTEIN; TRANSFORMING GROWTH FACTOR BETA; UNCLASSIFIED DRUG; PEPTIDE LIBRARY;

ARTICLE; ASSAY; CANCER INHIBITION; CONTROLLED STUDY; DRUG SCREENING; HUMAN; HUMAN CELL; IN SITU PROXIMITY LIGATION ASSAY; IN VITRO STUDY; INTRACELLULAR SIGNALING; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; TUMOR CELL; DRUG EFFECTS; MCF-7 CELL LINE; METABOLISM; PEPTIDE LIBRARY; PHOSPHORYLATION; TUMOR CELL LINE;

CELL LINE, TUMOR; DRUG SCREENING ASSAYS, ANTITUMOR; HUMANS; MCF-7 CELLS; PEPTIDE LIBRARY; PHOSPHORYLATION; SIGNAL TRANSDUCTION; SINGLE-CHAIN ANTIBODIES; SMAD PROTEINS; TRANSFORMING GROWTH FACTOR BETA;

EID: 84973293249     PISSN: 15359476     EISSN: 15359484     Source Type: Journal    
DOI: 10.1074/mcp.M115.055756     Document Type: Article

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