Improved microtitre plate production of single chain Fv fragments in Escherichia coli

New Biotechnology

Volumn 25, Issue 6, 2009, Pages 424-428

  Hust, Michael ^a   Steinwand, Miriam ^a   Al Halabi, Laila ^a   Helmsing, Saskia ^a   Schirrmann, Thomas ^a   Dübel, Stefan ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

96-WELL; ANTIBODY EXPRESSION; ANTIBODY FRAGMENT; FUNCTIONAL GENOMICS; IN-VITRO; MICROTITRE PLATE; OPTIMAL PARAMETER; PHAGE DISPLAY; POST-TRANSLATIONAL MODIFICATIONS; SINGLE-CHAIN FV; SPECIFIC DETECTION; STANDARD METHOD;

CLONING; MONOCLONAL ANTIBODIES;

ESCHERICHIA COLI;

SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY DETECTION; ANTIBODY PRODUCTION; ARTICLE; ESCHERICHIA COLI; FUNCTIONAL GENOMICS; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN PROCESSING;

BACTERIAL PROTEINS; CELL CULTURE TECHNIQUES; ESCHERICHIA COLI; IMMUNOGLOBULIN VARIABLE REGION; PEPTIDE LIBRARY;

ESCHERICHIA COLI;

EID: 70449524544     PISSN: 18716784     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.nbt.2009.03.004     Document Type: Article

References (39)

1. Taussig M.J., et al. ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat. Methods 4 (2007) 13-17
2. Sørensen M.D., et al. Severe acute respiratory syndrome (SARS): development of diagnostics and antivirals. Ann. N. Y. Acad. Sci. 1067 (2006) 500-505
3. Dübel S. Recombinant therapeutic antibodies. Appl. Microbiol. Biotechnol. 74 (2007) 723-729
4. Winter G., et al., Hoogenboom H.R. Making antibodies by phage display technology. Annu. Rev. Immunol. 12 (1994) 433-455
5. Winter G., and Milstein C. Man-made antibodies. Nature 349 (1991) 293-299
6. Hoogenboom H.R. Selecting and screening recombinant antibody libraries. Nat. Biotechnol. 23 (2005) 1105-1116
7. Schirrmann T., et al. Production systems for recombinant antibodies. Front. Biosci. 13 (2008) 4576-4594
8. Boel E., et al. Functional human monoclonal antibodies of all isotypes constructed from phage display library-derived single-chain Fv antibody fragments. J. Immunol. Methods 239 (2000) 153-166
9. Kirsch M., et al. Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV). BMC Biotechnol. 8 (2008) 66
10. Dübel S., and Kontermann R.E. Recombinant antibodies. Antibody Engineering (2001), Springer Verlag, Heidelberg/New York pp. 3-16
11. Hust M., and Dübel S. Mating antibody phage display with proteomics. Trends Biotechnol. 22 (2004) 8-14
12. Goshima N., et al. Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nat. Methods 5 (2008) 1011-1017
13. Langlais C., et al. A systematic approach for testing expression of human full-length proteins in cell-free expression systems. BMC Biotechnol. 7 (2007) 64
14. Rolfs A., et al. Production and sequence validation of a complete full length ORF collection for the pathogenic bacterium Vibrio cholerae. Proc. Natl. Acad. Sci. U. S. A. 105 (2008) 4364-4369
15. Konthur Z., et al. Perspectives for systematic in vitro antibody generation. Gene 364 (2005) 19-29
16. Hallborn J., and Carlsson R. Automated screening procedure for high-throughput generation of antibody fragments. Biotechniques Suppl. (2002) 30-37
17. Buckler D.R., et al. Screening isolates from antibody phage-display libraries. Drug Discov. Today 13 (2008) 318-324
18. Hust M., et al. Single chain Fab (scFab) fragment. BMC Biotechnol. 7 (2007) 14
19. Thie H., et al. SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli. Nat. Biotechnol. 25 (2008) 49-54
20. Skerra A. A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments. Gene 141 (1994) 79-84
21. Sambrook J., and Russell D. Molecular Cloning: A Laboratory Manual. 3rd edn (2001), Cold Spring Harbor Laboratory Press, New York
22. Hust M., Toleikis L., and Dübel S. Antibody phage display. Handbook of Therapeutic Antibodies (2007), Wiley-VCH, Weinheim
23. Pelat T., et al. High-affinity, human antibody-like antibody fragment (single-chain variable fragment) neutralizing the lethal factor (LF) of Bacillus anthracis by inhibiting protective antigen-LF complex formation. Antimicrob. Agents Chemother. 51 (2007) 2758-2764
24. Schlapschy M., et al. A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Protein Eng. Des. Sel. 19 (2006) 385-390
25. He M., and Taussig M.J. Antibody-ribosome-mRNA (ARM) complexes as efficient selection particles for in vitro display and evolution of antibody combining sites. Nucleic Acids Res. 25 (1997) 5132-5134
26. Wang M., and He M. The rapid discovery of engineered antibodies. IDrugs 10 (2007) 562-565
27. Goffinet M., et al. Identification of a GTP-bound Rho specific scFv molecular sensor by phage display selection. BMC Biotechnol. 8 (2008) 34
28. Jensen K.B., et al. Functional improvement of antibody fragments using a novel phage coat protein III fusion system. Biochem. Biophys. Res. Commun. 298 (2002) 566-573
29. Hust M., et al. Selection of recombinant antibodies from antibody gene libraries. Methods Mol. Biol. 408 (2007) 243-255
30. Hoet R.M., et al. Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity. Nat. Biotechnol. 23 (2005) 344-348
31. Garrard L.J., et al. Fab assembly and enrichment in a monovalent phage display system. Biotechnology (N. Y.) 9 (1991) 1373-1377
32. Iba Y., et al. Expression vectors for the introduction of highly diverged sequences into the six complementarity-determining regions of an antibody. Gene 194 (1997) 35-46
33. Hoogenboom H.R., et al. Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 19 (1991) 4133-4137
34. Goletz S., et al. Selection of large diversities of antiidiotypic antibody fragments by phage display. J. Mol. Biol. 315 (2002) 1087-1097
35. Mallano A., et al. Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from Yeast. BMC Biotechnol. 8 (2008) 68
36. Kipriyanov S.M., et al. High level production of soluble single chain antibodies in small-scale Escherichia coli cultures. J. Immunol. Methods 200 (1997) 69-77
37. Yang J., et al. One hundred seventy-fold increase in excretion of an FV fragment-tumor necrosis factor alpha fusion protein (sFV/TNF-alpha) from Escherichia coli caused by the synergistic effects of glycine and Triton X-100. Appl. Environ. Microbiol. 64 (1998) 2869-2874
38. Corisdeo S., and Wang B. Functional expression and display of an antibody Fab fragment in Escherichia coli: study of vector designs and culture conditions. Protein Expr. Purif. 34 (2004) 270-279
39. Kirsch M., et al. Parameters affecting the display of antibodies on phage. J. Immunol. Methods 301 (2005) 173-185