A protocol for exosome isolation and characterization: Evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods

Methods in Molecular Biology

Volumn 1295, Issue , 2015, Pages 179-209

  Greening, David W ^a   Xu, Rong ^a   Ji, Hong ^a   Tauro, Bow J ^a   Simpson, Richard J ^a  

^a NONE

Author keywords

Density; EpCAM; Exosome; Extracellular vesicle; Immunoaffinity capture; Isolation; Proteomics; Protocol; Purification; Shed microvesicles

Indexed keywords

EPITHELIAL CELL ADHESION MOLECULE; IODIXANOL; PROTEIN;

ALKYLATION; ANTIBODY AFFINITY; BIOGENESIS; CANCER CELL CULTURE; CELL FRACTIONATION; CELL ISOLATION; CELL LINE; CELL LYSATE; CELL SUSPENSION; CHEMISTRY; COLORECTAL CANCER CELL LINE; CONFERENCE PAPER; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; DENSITY GRADIENT; DENSITY GRADIENT CENTRIFUGATION; EXOSOME; EXTRACTION; HUMAN; HUMAN CELL; IMMUNOAFFINITY CAPTURE; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOBLOTTING; INVESTIGATIVE PROCEDURES; ISOLATION AND PURIFICATION; MEMBRANE VESICLE; PRIORITY JOURNAL; PROCEDURES; PROTEIN ANALYSIS; PROTEOMICS; PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TANDEM MASS SPECTROMETRY; TRANSMISSION ELECTRON MICROSCOPY; ULTRACENTRIFUGATION; WESTERN BLOTTING;

CELL FRACTIONATION; CELL LINE; CENTRIFUGATION, DENSITY GRADIENT; CHEMISTRY; EXOSOMES; HUMANS; IMMUNOCHROMATOGRAPHY; ISOLATION AND PURIFICATION; METHODS; PROTEINS; PROTEOMICS; TANDEM MASS SPECTROMETRY;

EID: 84964265534     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2550-6_15     Document Type: Conference Paper

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