Massive clonal selection and transiently contributing clones during expansion of mesenchymal stem cell cultures revealed by lentiviral rgb-barcode technology

Stem Cells Translational Medicine

Volumn 5, Issue 5, 2016, Pages 591-601

  Selich, Anton ^a   Daudert, Jannik ^a   Hass, Ralf ^a   Philipp, Friederike ^a,b   Von Kaisenberg, Constantin ^a   Paul, Gabi ^a   Cornils, Kerstin ^c   Fehse, Boris ^c   Rittinghausen, Susanne ^b   Schambach, Axel ^a,d   Rothe, Michael ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b FRAUNHOFER INSTITUTE FOR TOXICOLOGY AND EXPERIMENTAL MEDICINE   (Germany)

^c UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^d HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Clonal evolution; DNA barcoding; Mesenchymal stem cells; Reproducibility of results; Stem cell niche; Umbilical cord

Indexed keywords

FLUORESCENT DYE; RGB MARKING; UNCLASSIFIED DRUG;

ARTICLE; CELL ASSAY; CELL CLONE; CELL LABELING; CELL SUBPOPULATION; CONTROLLED STUDY; DNA BARCODING; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENE DOSAGE; HIGH THROUGHPUT SEQUENCING; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MESENCHYMAL STEM CELL; MONOLAYER CULTURE; STEM CELL CULTURE; STEM CELL EXPANSION; TUBE FORMATION ASSAY; UMBILICAL CORD; VIRAL GENE DELIVERY SYSTEM; CELL AGING; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CLONAL EVOLUTION; CYTOLOGY; FEMALE; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSDUCTION; GENETICS; GENOTYPE; LENTIVIRINAE; MESENCHYMAL STROMA CELL; METABOLISM; PHENOTYPE; PHYSIOLOGY; PREGNANCY; PROCEDURES; TIME FACTOR;

CELL AGING; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; CLONAL EVOLUTION; CLONE CELLS; DNA BARCODING, TAXONOMIC; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENETIC VECTORS; GENOTYPE; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; LENTIVIRUS; MESENCHYMAL STROMAL CELLS; MICROSCOPY, FLUORESCENCE; PHENOTYPE; PREGNANCY; TIME FACTORS; TRANSDUCTION, GENETIC; UMBILICAL CORD;

EID: 84964253294     PISSN: 21576564     EISSN: 21576580     Source Type: Journal    
DOI: 10.5966/sctm.2015-0176     Document Type: Article

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