Epidermal growth factor improves lentivirus vector gene transfer into primary mouse hepatocytes

Gene Therapy

Volumn 19, Issue 4, 2012, Pages 425-434

  Rothe, M ^a,b   Rittelmeyer, I ^a,b   Iken, M ^a,b   Rudrich U ^a,b   Schambach, A ^a   Glage, S ^a   Manns, M P ^a   Baum, C ^a   Bock, M ^a   Ott, M ^a,b   Modlich, U ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b INSTITUTE OF INFECTION IMMUNOLOGY   (Germany)

Author keywords

EGF; ex vivo gene therapy; hepatocytes; lentiviral vector; transduction

Indexed keywords

EPIDERMAL GROWTH FACTOR; LENTIVIRUS VECTOR; RETROVIRUS VECTOR; SCATTER FACTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL ADHESION; CELL CULTURE; CELL PROLIFERATION; CONTROLLED STUDY; EX VIVO GENE TRANSFER; GENE TRANSFER; IN VITRO STUDY; LIVER; LIVER CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; RETROVIRUS; VIRAL GENE DELIVERY SYSTEM;

ANIMALS; CELLS, CULTURED; CULTURE MEDIA; EPIDERMAL GROWTH FACTOR; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HEPATOCYTE GROWTH FACTOR; HEPATOCYTES; LENTIVIRUS; MICE; MICE, INBRED C57BL; TRANSDUCTION, GENETIC;

ANIMALIA; LENTIVIRUS;

EID: 84859589642     PISSN: 09697128     EISSN: 14765462     Source Type: Journal    
DOI: 10.1038/gt.2011.117     Document Type: Article

References (40)

1. Perera MT, Mirza DF, Elias E. Liver transplantation: Issues for the next 20 years. J Gastroenterol Hepatol 2009; 24 (Suppl 3): S124-S131.
2. Grossman M, Rader DJ, Muller DW, Kolansky DM, Kozarsky K, Clark 3rd BJ et al. A pilot study of ex vivo gene therapy for homozygous familial hypercholesterolaemia. Nat Med 1995; 1: 1148-1154.
3. Amado RG, Chen IS. Lentiviral vectors - the promise of gene therapy within reach? Science 1999; 285: 674-676.
4. Zychlinski D, Schambach A, Modlich U, Maetzig T, Meyer J, Grassman E et al. Physiological promoters reduce the genotoxic risk of integrating gene vectors. Mol Ther 2008; 16: 718-725. (Pubitemid 351426173)
5. Montini E, Cesana D, Schmidt M, Sanvito F, Bartholomae CC, Ranzani M et al. The genotoxic potential of retroviral vectors is strongly modulated by vector design and integration site selection in a mouse model of HSC gene therapy. J Clin Invest 2009; 119: 964-975.
6. Modlich U, Navarro S, Zychlinski D, Maetzig T, Knoess S, Brugman MH et al. Insertional transformation of hematopoietic cells by self-inactivating lentiviral and gammaretroviral vectors. Mol Ther 2009; 17: 1919-1928.
7. Weber A, Groyer-Picard MT, Franco D, Dagher I. Hepatocyte transplantation in animal models. Liver Transpl 2009; 15: 7-14.
8. Nguyen TH, Mainot S, Lainas P, Groyer-Picard MT, Franco D, Dagher I et al. Ex vivo liver-directed gene therapy for the treatment of metabolic diseases: advances in hepatocyte transplantation and retroviral vectors. Curr Gene Ther 2009; 9: 136-149.
9. Kerr TA, Davidson NO. Therapeutic RNA manipulation in liver disease. Hepatology 2010; 51: 1055-1061.
10. Nguyen TH, Oberholzer J, Birraux J, Majno P, Morel P, Trono D. Highly efficient lentiviral vector-mediated transduction of nondividing, fully reimplantable primary hepatocytes. Mol Ther 2002; 6: 199-209. (Pubitemid 36336127)
11. Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc Natl Acad Sci USA 1993; 90: 8033-8037. (Pubitemid 23260849)
12. Ohnishi N, Itoh K, Itoh Y, Baum C, Higashitsuji H, Yamaguchi K et al. High expression of transgenes mediated by hybrid retroviral vectors in hepatocytes: comparison of promoters from murine retroviruses in vitro and in vivo. Gene Therapy 2002; 9: 303-306. (Pubitemid 34256906)
13. Castell JV, Gomez-Lechon MJ. Liver cell culture techniques. Methods Mol Biol 2009; 481: 35-46.
14. Selden C, Mellor N, Rees M, Laurson J, Kirwan M, Escors D et al. Growth factors improve gene expression after lentiviral transduction in human adult and fetal hepatocytes. J Gene Med 2007; 9: 67-76. (Pubitemid 46444484)
15. Schaudies RP, Savage Jr CR. Isolation of rat epidermal growth factor (r-EGF): chemical, biological immunological comparisons with mouse and human EGF. Comp Biochem Physiol B 1986; 84: 497-505.
16. Schambach A, Bohne J, Baum C, Hermann FG, Egerer L, von Laer D et al. Woodchuck hepatitis virus post-transcriptional regulatory element deleted from X protein and promoter sequences enhances retroviral vector titer and expression. Gene Therapy 2006; 13: 641-645.
17. Richman RA, Claus TH, Pilkis SJ, Friedman DL. Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes. Proc Natl Acad Sci USA 1976; 73: 3589-3593.
18. Gohda E, Tsubouchi H, Nakayama H, Hirono S, Sakiyama O, Takahashi K et al. Purification and partial characterization of hepatocyte growth factor from plasma of a patient with fulminant hepatic failure. J Clin Invest 1988; 81: 414-419. (Pubitemid 18075233)
19. Suzuki Y, Craigie R. The road to chromatin - nuclear entry of retroviruses. Nat Rev Microbiol 2007; 5: 187-196. (Pubitemid 46291278)
20. Miller DG, Adam MA, Miller AD. Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection. Mol Cell Biol 1990; 10: 4239-4242.
21. Mandal D, Prasad VR. Analysis of 2-LTR circle junctions of viral DNA in infected cells. Methods Mol Biol 2009; 485: 73-85.
22. Butler SL, Hansen MS, Bushman FD. A quantitative assay for HIV DNA integration in vivo. Nat Med 2001; 7: 631-634. (Pubitemid 32448333)
23. Tervo HM, Goffinet C, Keppler OT. Mouse T-cells restrict replication of human immunodeficiency virus at the level of integration. Retrovirology 2008; 5: 58.
24. Haridass D, Yuan Q, Becker PD, Cantz T, Iken M, Rothe M et al. Repopulation efficiencies of adult hepatocytes, fetal liver progenitor cells, and embryonic stem cell-derived hepatic cells in albumin-promoter-enhancer urokinase-type plasminogen activator mice. Am J Pathol 2009; 175: 1483-1492.
25. Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y. 'Green mice' as a source of ubiquitous green cells. FEBS Lett 1997; 407: 313-319. (Pubitemid 27199450)
26. Korin YD, Zack JA. Progression to the G1b phase of the cell cycle is required for completion of human immunodeficiency virus type 1 reverse transcription in T cells. J Virol 1998; 72: 3161-3168. (Pubitemid 28175559)
27. Funke S, Maisner A, Muhlebach MD, Koehl U, Grez M, Cattaneo R et al. Targeted cell entry of lentiviral vectors. Mol Ther 2008; 16: 1427-1436.
28. Desfarges S, Salin B, Calmels C, Andreola ML, Parissi V, Fournier M. HIV-1 integrase trafficking in S. cerevisiae: a useful model to dissect the microtubule network involvement of viral protein nuclear import. Yeast 2009; 26: 39-54.
29. Buchholz CJ, Muhlebach MD, Cichutek K. Lentiviral vectors with measles virus glycoproteins - dream team for gene transfer? Trends Biotechnol 2009; 27: 259-265.
30. Lo HW, Ali-Seyed M, Wu Y, Bartholomeusz G, Hsu SC, Hung MC. Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1. J Cell Biochem 2006; 98: 1570-1583. (Pubitemid 44141861)
31. Wang YN, Yamaguchi H, Hsu JM, Hung MC. Nuclear trafficking of the epidermal growth factor receptor family membrane proteins. Oncogene 2010; 29: 3997-4006.
32. O'Neill LS, Skinner AM, Woodward JA, Kurre P. Entry kinetics and cell-cell transmission of surface-bound retroviral vector particles. J Gene Med 2010; 12: 463-476.
33. Schambach A, Bohne J, Chandra S, Will E, Margison GP, Williams DA et al. Equal potency of gammaretroviral and lentiviral SIN vectors for expression of O6-methylguanine- DNA methyltransferase in hematopoietic cells. Mol Ther 2006; 13: 391-400. (Pubitemid 43056905)
34. Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D et al. A third-generation lentivirus vector with a conditional packaging system. J Virol 1998; 72: 8463-8471. (Pubitemid 28471781)
35. Schambach A, Mueller D, Galla M, Verstegen MM, Wagemaker G, Loew R et al. Overcoming promoter competition in packaging cells improves production of self-inactivating retroviral vectors. Gene Therapy 2006; 13: 1524-1533. (Pubitemid 44567149)
36. Kutner RH, Zhang XY, Reiser J. Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors. Nat Protoc 2009; 4: 495-505.
37. Seglen PO. Hepatocyte suspensions and cultures as tools in experimental carcinogenesis. J Toxicol Environ Health 1979; 5: 551-560. (Pubitemid 9214773)
38. Sandgren EP, Palmiter RD, Heckel JL, Daugherty CC, Brinster RL, Degen JL. Complete hepatic regeneration after somatic deletion of an albumin-plasminogen activator transgene. Cell 1991; 66: 245-256. (Pubitemid 121001363)
39. Morosan S, Hez-Deroubaix S, Lunel F, Renia L, Giannini C, Van Rooijen N et al. Liver-stage development of Plasmodium falciparum, in a humanized mouse model. J Infect Dis 2006; 193: 996-1004.
40. Silva-Barbosa SD, Butler-Browne GS, Di Santo JP, Mouly V. Comparative analysis of genetically engineered immunodeficient mouse strains as recipients for human myoblast transplantation. Cell Transplant 2005; 14: 457-467. (Pubitemid 41566352)