메뉴 건너뛰기




Volumn 585, Issue 1, 2016, Pages 135-142

Validation of RT-qPCR reference genes and determination of Robo4 expression levels in human retinal endothelial cells under hypoxia and/or hyperglycemia

Author keywords

Diabetic Retinopathy; HREC; Hyperglycemia; Hypoxia; Reference Genes; Robo4

Indexed keywords

5 AMINOLEVULINATE SYNTHASE; ACTIN; BETA 2 MICROGLOBULIN; BETA GLUCURONIDASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; PEPTIDYLPROLYL ISOMERASE; PORPHOBILINOGEN DEAMINASE; PROTEIN; RIBOSOME PROTEIN; RNA; RNA 18S; ROUNDABOUT4 PROTEIN; TATA BINDING PROTEIN; TRANSFERRIN RECEPTOR; UNCLASSIFIED DRUG; CELL SURFACE RECEPTOR; MESSENGER RNA; PUM1 PROTEIN, HUMAN; RNA BINDING PROTEIN; ROBO4 PROTEIN, HUMAN; TBP PROTEIN, HUMAN;

EID: 84963700025     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2016.03.047     Document Type: Article
Times cited : (20)

References (36)
  • 1
    • 84922271504 scopus 로고    scopus 로고
    • Identification of suitable reference genes for gene expression studies using quantitative polymerase chain reaction in lung cancer in vitro
    • Ali H., Du Z., Li X., Yang Q., Zhang Y.C., et al. Identification of suitable reference genes for gene expression studies using quantitative polymerase chain reaction in lung cancer in vitro. Mol. Med. Rep. 2015, 11:3767-3773.
    • (2015) Mol. Med. Rep. , vol.11 , pp. 3767-3773
    • Ali, H.1    Du, Z.2    Li, X.3    Yang, Q.4    Zhang, Y.C.5
  • 2
    • 3442876969 scopus 로고    scopus 로고
    • Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    • Andersen C.L., Jensen J.L., Orntoft T.F. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004, 64:5245-5250.
    • (2004) Cancer Res. , vol.64 , pp. 5245-5250
    • Andersen, C.L.1    Jensen, J.L.2    Orntoft, T.F.3
  • 3
    • 80053471739 scopus 로고    scopus 로고
    • Hypoxia and oxidative stress in the causation of diabetic retinopathy
    • Arden G.B., Sivaprasad S. Hypoxia and oxidative stress in the causation of diabetic retinopathy. Curr. Diabetes Rev. 2011, 7:291-304.
    • (2011) Curr. Diabetes Rev. , vol.7 , pp. 291-304
    • Arden, G.B.1    Sivaprasad, S.2
  • 4
    • 80054094641 scopus 로고    scopus 로고
    • Reference gene validation for qPCR on normoxia- and hypoxia-cultured human dermal fibroblasts exposed to UVA: is beta-actin a reliable normalizer for photoaging studies?
    • Bruge F., Venditti E., Tiano L., Littarru G.P., Damiani E. Reference gene validation for qPCR on normoxia- and hypoxia-cultured human dermal fibroblasts exposed to UVA: is beta-actin a reliable normalizer for photoaging studies?. J. Biotechnol. 2011, 156:153-162.
    • (2011) J. Biotechnol. , vol.156 , pp. 153-162
    • Bruge, F.1    Venditti, E.2    Tiano, L.3    Littarru, G.P.4    Damiani, E.5
  • 6
    • 84872046851 scopus 로고    scopus 로고
    • Effect of Robo4 on retinal endothelial permeability
    • Cheng Y., Liu Z., Wang S., Wang C., Qi S., et al. Effect of Robo4 on retinal endothelial permeability. Curr. Eye Res. 2013, 38:128-136.
    • (2013) Curr. Eye Res. , vol.38 , pp. 128-136
    • Cheng, Y.1    Liu, Z.2    Wang, S.3    Wang, C.4    Qi, S.5
  • 7
    • 79960731769 scopus 로고    scopus 로고
    • High glucose-induced altered basement membrane composition and structure increases trans-endothelial permeability: implications for diabetic retinopathy
    • Chronopoulos A., Trudeau K., Roy S., Huang H., Vinores S.A., et al. High glucose-induced altered basement membrane composition and structure increases trans-endothelial permeability: implications for diabetic retinopathy. Curr. Eye Res. 2011, 36:747-753.
    • (2011) Curr. Eye Res. , vol.36 , pp. 747-753
    • Chronopoulos, A.1    Trudeau, K.2    Roy, S.3    Huang, H.4    Vinores, S.A.5
  • 9
    • 84896968297 scopus 로고    scopus 로고
    • Capacity of aflibercept to counteract VEGF-stimulated abnormal behavior of retinal microvascular endothelial cells
    • Deissler H.L., Lang G.K., Lang G.E. Capacity of aflibercept to counteract VEGF-stimulated abnormal behavior of retinal microvascular endothelial cells. Exp. Eye Res. 2014, 122:20-31.
    • (2014) Exp. Eye Res. , vol.122 , pp. 20-31
    • Deissler, H.L.1    Lang, G.K.2    Lang, G.E.3
  • 10
    • 3142615272 scopus 로고    scopus 로고
    • Validation of housekeeping genes for normalizing RNA expression in real-time PCR
    • 116, 118-119)
    • Dheda K., Huggett J.F., Bustin S.A., Johnson M.A., Rook G., et al. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques 2004, 37. (112-114, 116, 118-119).
    • (2004) Biotechniques , vol.37 , pp. 112-114
    • Dheda, K.1    Huggett, J.F.2    Bustin, S.A.3    Johnson, M.A.4    Rook, G.5
  • 11
  • 12
    • 84991212416 scopus 로고    scopus 로고
    • Selection of suitable reference genes for reverse transcription-quantitative polymerase chain reaction analysis of neuronal cells differentiated from bone mesenchymal stem cells
    • He Y.X., Zhang Y., Yang Q., Wang C., Su G. Selection of suitable reference genes for reverse transcription-quantitative polymerase chain reaction analysis of neuronal cells differentiated from bone mesenchymal stem cells. Mol. Med. Rep. 2015, 12:2291-2300.
    • (2015) Mol. Med. Rep. , vol.12 , pp. 2291-2300
    • He, Y.X.1    Zhang, Y.2    Yang, Q.3    Wang, C.4    Su, G.5
  • 13
    • 66649105475 scopus 로고    scopus 로고
    • Expression of Robo4 in the fibrovascular membranes from patients with proliferative diabetic retinopathy and its role in RF/6A and RPE cells
    • Huang L., Yu W., Li X., Xu Y., Niu L., et al. Expression of Robo4 in the fibrovascular membranes from patients with proliferative diabetic retinopathy and its role in RF/6A and RPE cells. Mol. Vis. 2009, 15:1057-1069.
    • (2009) Mol. Vis. , vol.15 , pp. 1057-1069
    • Huang, L.1    Yu, W.2    Li, X.3    Xu, Y.4    Niu, L.5
  • 14
    • 0036209165 scopus 로고    scopus 로고
    • Magic roundabout is a new member of the roundabout receptor family that is endothelial specific and expressed at sites of active angiogenesis
    • Huminiecki L., Gorn M., Suchting S., Poulsom R., Bicknell R. Magic roundabout is a new member of the roundabout receptor family that is endothelial specific and expressed at sites of active angiogenesis. Genomics 2002, 79:547-552.
    • (2002) Genomics , vol.79 , pp. 547-552
    • Huminiecki, L.1    Gorn, M.2    Suchting, S.3    Poulsom, R.4    Bicknell, R.5
  • 15
    • 77955870163 scopus 로고    scopus 로고
    • Gene expression profile of hyperoxic and hypoxic retinas in a mouse model of oxygen-induced retinopathy
    • Ishikawa K., Yoshida S., Kadota K., Nakamura T., Niiro H., et al. Gene expression profile of hyperoxic and hypoxic retinas in a mouse model of oxygen-induced retinopathy. Invest. Ophthalmol. Vis. Sci. 2010, 51:4307-4319.
    • (2010) Invest. Ophthalmol. Vis. Sci. , vol.51 , pp. 4307-4319
    • Ishikawa, K.1    Yoshida, S.2    Kadota, K.3    Nakamura, T.4    Niiro, H.5
  • 16
    • 41849131420 scopus 로고    scopus 로고
    • Robo4 stabilizes the vascular network by inhibiting pathologic angiogenesis and endothelial hyperpermeability
    • Jones C.A., London N.R., Chen H., Park K.W., Sauvaget D., et al. Robo4 stabilizes the vascular network by inhibiting pathologic angiogenesis and endothelial hyperpermeability. Nat. Med. 2008, 14:448-453.
    • (2008) Nat. Med. , vol.14 , pp. 448-453
    • Jones, C.A.1    London, N.R.2    Chen, H.3    Park, K.W.4    Sauvaget, D.5
  • 17
    • 70449529782 scopus 로고    scopus 로고
    • Slit2-Robo4 signalling promotes vascular stability by blocking Arf6 activity
    • Jones C.A., Nishiya N., London N.R., Zhu W., Sorensen L.K., et al. Slit2-Robo4 signalling promotes vascular stability by blocking Arf6 activity. Nat. Cell Biol. 2009, 11:1325-1331.
    • (2009) Nat. Cell Biol. , vol.11 , pp. 1325-1331
    • Jones, C.A.1    Nishiya, N.2    London, N.R.3    Zhu, W.4    Sorensen, L.K.5
  • 18
    • 0021163570 scopus 로고
    • Urethane-induced rat retinopathy. Plasticity of the blood-retinal barrier in disease
    • Korte G.E., Bellhorn R.W., Burns M.S. Urethane-induced rat retinopathy. Plasticity of the blood-retinal barrier in disease. Invest. Ophthalmol. Vis. Sci. 1984, 25:1027-1034.
    • (1984) Invest. Ophthalmol. Vis. Sci. , vol.25 , pp. 1027-1034
    • Korte, G.E.1    Bellhorn, R.W.2    Burns, M.S.3
  • 19
    • 84886734511 scopus 로고    scopus 로고
    • Reference genes in real-time PCR
    • Kozera B., Rapacz M. Reference genes in real-time PCR. J. Appl. Genet. 2013, 54:391-406.
    • (2013) J. Appl. Genet. , vol.54 , pp. 391-406
    • Kozera, B.1    Rapacz, M.2
  • 20
    • 84957430221 scopus 로고    scopus 로고
    • Identification and validation of reference genes for quantitative RT-PCR analysis of retinal pigment epithelium cells under hypoxia and/or hyperglycemia
    • Liu X., Xie J., Liu Z., Gong Q., Tian R., et al. Identification and validation of reference genes for quantitative RT-PCR analysis of retinal pigment epithelium cells under hypoxia and/or hyperglycemia. Gene 2016, 580:41-46.
    • (2016) Gene , vol.580 , pp. 41-46
    • Liu, X.1    Xie, J.2    Liu, Z.3    Gong, Q.4    Tian, R.5
  • 21
    • 7044229733 scopus 로고    scopus 로고
    • Interactions between hyperglycemia and hypoxia: implications for diabetic retinopathy
    • Nyengaard J.R., Ido Y., Kilo C., Williamson J.R. Interactions between hyperglycemia and hypoxia: implications for diabetic retinopathy. Diabetes 2004, 53:2931-2938.
    • (2004) Diabetes , vol.53 , pp. 2931-2938
    • Nyengaard, J.R.1    Ido, Y.2    Kilo, C.3    Williamson, J.R.4
  • 22
    • 1842483281 scopus 로고    scopus 로고
    • Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations
    • Pfaffl M.W., Tichopad A., Prgomet C., Neuvians T.P. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 2004, 26:509-515.
    • (2004) Biotechnol. Lett. , vol.26 , pp. 509-515
    • Pfaffl, M.W.1    Tichopad, A.2    Prgomet, C.3    Neuvians, T.P.4
  • 23
    • 79955953053 scopus 로고    scopus 로고
    • A potential role for angiopoietin 2 in the regulation of the blood-retinal barrier in diabetic retinopathy
    • Rangasamy S., Srinivasan R., Maestas J., McGuire P.G., Das A. A potential role for angiopoietin 2 in the regulation of the blood-retinal barrier in diabetic retinopathy. Invest. Ophthalmol. Vis. Sci. 2011, 52:3784-3791.
    • (2011) Invest. Ophthalmol. Vis. Sci. , vol.52 , pp. 3784-3791
    • Rangasamy, S.1    Srinivasan, R.2    Maestas, J.3    McGuire, P.G.4    Das, A.5
  • 24
    • 84896296860 scopus 로고    scopus 로고
    • The effect of hyperglycaemia on permeability and the expression of junctional complex molecules in human retinal and choroidal endothelial cells
    • Saker S., Stewart E.A., Browning A.C., Allen C.L., Amoaku W.M. The effect of hyperglycaemia on permeability and the expression of junctional complex molecules in human retinal and choroidal endothelial cells. Exp. Eye Res. 2014, 121:161-167.
    • (2014) Exp. Eye Res. , vol.121 , pp. 161-167
    • Saker, S.1    Stewart, E.A.2    Browning, A.C.3    Allen, C.L.4    Amoaku, W.M.5
  • 25
    • 0034694128 scopus 로고    scopus 로고
    • Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR
    • Schmittgen T.D., Zakrajsek B.A. Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J. Biochem. Biophys. Methods 2000, 46:69-81.
    • (2000) J. Biochem. Biophys. Methods , vol.46 , pp. 69-81
    • Schmittgen, T.D.1    Zakrajsek, B.A.2
  • 28
    • 84939546371 scopus 로고    scopus 로고
    • Investigation of the regulation of Roundabout4 (Robo4) by hypoxia-inducible factor-1alpha in microvascular endothelial cells
    • Tian R., Liu Z.X., Zhang H., Fang X.X., Wang C.G., et al. Investigation of the regulation of Roundabout4 (Robo4) by hypoxia-inducible factor-1alpha in microvascular endothelial cells. Invest. Ophthalmol. Vis. Sci. 2015.
    • (2015) Invest. Ophthalmol. Vis. Sci.
    • Tian, R.1    Liu, Z.X.2    Zhang, H.3    Fang, X.X.4    Wang, C.G.5
  • 29
    • 84876579314 scopus 로고    scopus 로고
    • Diosmin alleviates retinal edema by protecting the blood-retinal barrier and reducing retinal vascular permeability during ischemia/reperfusion injury
    • Tong N., Zhang Z., Zhang W., Qiu Y., Gong Y., et al. Diosmin alleviates retinal edema by protecting the blood-retinal barrier and reducing retinal vascular permeability during ischemia/reperfusion injury. PLoS One 2013, 8.
    • (2013) PLoS One , vol.8
    • Tong, N.1    Zhang, Z.2    Zhang, W.3    Qiu, Y.4    Gong, Y.5
  • 30
    • 77954617065 scopus 로고    scopus 로고
    • High glucose disrupts mitochondrial morphology in retinal endothelial cells: implications for diabetic retinopathy
    • Trudeau K., Molina A.J., Guo W., Roy S. High glucose disrupts mitochondrial morphology in retinal endothelial cells: implications for diabetic retinopathy. Am. J. Pathol. 2010, 177:447-455.
    • (2010) Am. J. Pathol. , vol.177 , pp. 447-455
    • Trudeau, K.1    Molina, A.J.2    Guo, W.3    Roy, S.4
  • 31
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3.
    • (2002) Genome Biol. , vol.3
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3    Poppe, B.4    Van Roy, N.5
  • 33
    • 84868157972 scopus 로고    scopus 로고
    • Stability of endogenous reference genes in postmortem human brains for normalization of quantitative real-time PCR data: comprehensive evaluation using geNorm, NormFinder, and BestKeeper
    • Wang Q., Ishikawa T., Michiue T., Zhu B.L., Guan D.W., et al. Stability of endogenous reference genes in postmortem human brains for normalization of quantitative real-time PCR data: comprehensive evaluation using geNorm, NormFinder, and BestKeeper. Int. J. Legal Med. 2012, 126:943-952.
    • (2012) Int. J. Legal Med. , vol.126 , pp. 943-952
    • Wang, Q.1    Ishikawa, T.2    Michiue, T.3    Zhu, B.L.4    Guan, D.W.5
  • 34
    • 85006246253 scopus 로고    scopus 로고
    • High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy
    • Wang L., Wang J., Fang J., Zhou H., Liu X., et al. High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy. Diabetol. Metab. Syndr. 2015, 7:89.
    • (2015) Diabetol. Metab. Syndr. , vol.7 , pp. 89
    • Wang, L.1    Wang, J.2    Fang, J.3    Zhou, H.4    Liu, X.5
  • 35
    • 84893850202 scopus 로고    scopus 로고
    • O-GlcNAcylation under hypoxic conditions and its effects on the blood-retinal barrier in diabetic retinopathy
    • Xu C., Liu G., Liu X., Wang F. O-GlcNAcylation under hypoxic conditions and its effects on the blood-retinal barrier in diabetic retinopathy. Int. J. Mol. Med. 2014, 33:624-632.
    • (2014) Int. J. Mol. Med. , vol.33 , pp. 624-632
    • Xu, C.1    Liu, G.2    Liu, X.3    Wang, F.4
  • 36
    • 84933041675 scopus 로고    scopus 로고
    • Protective factors in diabetic retinopathy: focus on blood-retinal barrier
    • Zhang C., Wang H., Nie J., Wang F. Protective factors in diabetic retinopathy: focus on blood-retinal barrier. Discov. Med. 2014, 18:105-112.
    • (2014) Discov. Med. , vol.18 , pp. 105-112
    • Zhang, C.1    Wang, H.2    Nie, J.3    Wang, F.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.