Cellulase adsorption on lignin: A roadblock for economic hydrolysis of biomass

Renewable Energy

Volumn 98, Issue , 2016, Pages 29-42

  Saini, Jitendra Kumar ^a   Patel, Anil Kumar ^a   Adsul, Mukund ^a   Singhania, Reeta Rani ^a  

^a INDIAN OIL CORPORATION LIMITED   (India)

Author keywords

Biomass; Cellulase; Enzyme inhibition; Hydrolysis; Lignin; Pretreatment

Indexed keywords

BIOETHANOL; CELLULOSE; ENZYME INHIBITION; ETHANOL; HYDROLYSIS; LIGNIN; SACCHARIFICATION;

BIO-ETHANOL PRODUCTION; CELLULASE; CELLULASE ADSORPTION; COST OF ENZYMES; ENZYMATIC SACCHARIFICATION; LIGNOCELLULOSIC BIOMASS; PRE-TREATMENT;

BIOMASS;

ADSORPTION; BIOFUEL; BIOMASS; CELLULOSE; ENZYME; ENZYME ACTIVITY; HYDROLYSIS; LIGNIN;

EID: 84962028317     PISSN: 09601481     EISSN: 18790682     Source Type: Journal    
DOI: 10.1016/j.renene.2016.03.089     Document Type: Article

References (140)

1. [1] Ooshima, H., Burns, D.S., Converse, A.O., Adsorption of cellulase from Trichoderma reesei on cellulose and lignacious residue in wood pretreated by dilute sulfuric-acid with explosive decompression. Biotechnol. Bioeng. 36:5 (1990), 446–452.
2. [2] Saini, J.K., Saini, R., Tewari, L., Lignocellulosic agriculture wastes as biomass feedstocks for second-generation bioethanol production: concepts and recent developments. 3 Biotech., 5, 2015 337e353 http://dx.doi.org/10.1007/s13205-014-0246-5.
3. [3] Koshijima, T., Watanabe, T., Yaku, F., Structure and properties of the lignin-carbohydrate polymer as an amphipathick substance. Glasser, W.G., Sarkanen, S., (eds.) Lignin Properties and Materials, 1998, American Chemical Society, Washington, DC, 11–28.
4. [4] http://web.nchu.edu.tw/pweb/users/taiwanfir/lesson/10476.pdf (accessed on 25th February 2016).
5. [5] Hatakeyama, T., Quinn, F.X., Thermal analysis, Fundamentals and Applications to Polymer Science. second ed., 1999, Wiley, UK.
6. [6] Yarbrough, J.M., Mittal, A., Mansfield, E., Taylor, L.E., Hobdey, S.E., Sammond, D.W., Bomble, Y.J., Crowley, M.F., Decker, S.R., Himmel, M.E., Vinzant, T.B., Yarbrough, et al. New perspective on glycoside hydrolase binding to lignin from pretreated corn stover. Biotechnol. Biofuels, 8, 2015, 214, 10.1186/s13068-015-0397-6.
7. [7] Hatakeyama, H., Nakamura, K., Hatakeyama, T., Studies on factors affecting the molecular motion of lignin and lignin-related polystyrene derivatives. Trans. Pulp Pap. Can. 81 (1980), 105–110.
8. [8] Hatakeyama, H., Nakano, J., Nuclear magnetic resonance studies on lignin in solid state. Tappi 53 (1970), 472–475.
9. [9] Hatakeyama, H., Hatakeyama, T., Interaction between water and hydrophilic polymers. Thermochim. Acta 308 (1998), 3–22.
10. [10] Nakamura, K., Hatakeyama, T., Hatakeyama, H., Differential scanning calorimetric studies on the glass transition temperature of polyhydroxystyrene derivatives containing sorbed water. Polymer 22 (1981), 473–476.
11. [11] Hatakeyama, T., Hirose, S., Hatakeyama, H., Differential scanning calorimetric studies on bound water in 1.4-dioxane acidolysis lignin. Macromol. Chem. 184 (1983), 1265–1274.
12. [12] Yano, S., Rigdahl, M., Kolseth, P., Ruvo, A., Water-induced softening lignosulfonates Part 1. Effect of molecular mass. Sven. Papperstidning 87 (1984), R170–R176.
13. [13] Yano, S., Rigdahl, M., Kolseth, P., Ruvo, A., Water-induced softening lignosulfonates Part 1. Effect of counter ion. Sven. Papperstidning 88 (1984), R10–R14.
14. [14] Hatakeyama, T., Nakamura, K., Hatakeyama, H., Determination of bound water content in polymers by DTA, DSC and TG. Thermochim. Acta 123 (1988), 153–161.
15. [15] Hatakeyama, H., Hatakeyama, T., Lignin structure, properties, and applications. Adv. Polym. Sci., 2009, 10.1007/12 2009 12 c Springer-Verlag Berlin Heidelberg.
16. [16] Pan, X.J., Xie, D., Gilkes, N., Gregg, D.J., Saddler, J.N., Strategies of enhance the enzymatic hydrolysis of pretreated softwood with high residual lignin content. Appl. Biochem. Biotechnol. 121–124 (2005), 1069–1079.
17. [17] Ximenes, E., Kim, Y., Mosier, N., Dien, B., Ladisch, M., Deactivation of cellulases by phenols. Enzyme Microb. Technol. 48 (2011), 54–60.
18. [18] Lee, D., Yu, A.H.C., Saddler, J.N., Evaluation of cellulase recycling strategies for the hydrolysis of lignocellulosic substrates. Biotechnol. Bioeng. 45 (1995), 328–336.
19. [19] Chernoglazov, V.M., Ermolova, O.V., Klyosov, A.A., Adsorption of high purity endo-1-4 glucanase frpm Trichoderma reesei on components of lignocellulosic materials: cellulose, lignin and xylan. Enzym. Microb. Technol. 10 (1988), 503–507.
20. [20] Sutcliffe, R., Saddler, J.N., The role of lignin in the adsorption of cellulases during enzymatic treatment of lignocellulosic material. Biotechnol. Bioeng. Symp. 17 (1986), 749–762.
21. [21] Mansfield, S.D., Mooney, C., Saddler, J.N., Substrate and enzyme characteristics that limit cellulose hydrolysis. Biotechnol. Prog. 15 (1999), 804–816.
22. [22] Hall, M., Bansal, P., Lee, J.H., Realff, M.J., Bommarius, A.S., Cellulose crystallinity—a key predictor of the enzymatic hydrolysis rate. FEBS J. 277:6 (2010), 1571–1582.
23. [23] Leu, S.Y., Zhu, J.Y., Substrate-related factors affecting enzymatic saccharification of lignocelluloses: our recent understanding. Bioenerg. Res. 6:2 (2013), 405–415.
24. [24] Cowling, E.B., Kirk, T.K., Properties of cellulose and lignocellulosic materials as substrates for enzymatic conversion processes. Biotech. Bioeng. Symp. 6 (1976), 95–123.
25. [25] Mansfield, S.D., Mooney, C., Saddler, J.N., Substrate and Enzyme Characteristics That limit cellulose hydrolysis. Biotechnol. Prog. 15 (1999), 804–816.
26. [26] Mandels, M., Reese, E.T., Dev. Ind. Microbiol. 5 (1964), 5–16.
27. [27] Adsul, M., Sharma, B., Singhania, R.R., Saini, J.K., Sharma, A., Mathur, A., Gupta, R., Tuli, D.K., Blending of cellulolytic enzyme preparations from different fungal sources for improved cellulose hydrolysis by increasing synergism. RSC Adv. 4 (2014), 44726–44732.
28. [28] Saini, J.K., Singhania, R.R., Satlewal, A., Saini, R., Gupta, R., Tuli, D.K., Mathur, A., Adsul, M., Improvement of wheat straw hydrolysis by Cellulolytic blends of two Penicillium spp. Renew. Energy, 2016, 10.1016/j.renene.2016.01.025 In Press.
29. [29] Din, N., Gilkes, N.R., Tekant, B., Miller, R.C. Jr., Warren, R.A.J., Kilburn, D.G., Nonhydrolytic disruption of cellulose fibres by the binding domain of a bacterial cellulase. Bio/Technology 9 (1991), 1096–1099.
30. [30] Teeri, T.T., Reinikainen, T., Ruohonen, L., Jones, T.A., Knowles, J.K.C., Domain function in Trichoderma reesei cellobiohydrolases. J. Biotechnol. 22:2 (1992), 169–176.
31. [31] Tomme, P., Van Tilbeurgh, H., Pettersson, G., VanDamme, J., Vandekerckhove, J., Knowles, J., Teeri, T., Claeyssens, M., Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis. Eur. J. Biochem. 170 (1988), 575–581.
32. [32] Kim, Y., Kreke, T., Ko, J.K., Ladisch, M.R., Hydrolysis-determining substrate characteristics in liquid hot water pretreated hardwood. Biotechnol. Bioeng. 112:4 (2015), 677–687.
33. [33] Zeng, Y.N., Zhao, S., Yang, S.H., Ding, S.Y., Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels. Curr. Opin. Biotechnol. 27 (2014), 38–45.
34. [34] Pan, X.J., Arato, C., Gilkes, N., Gregg, D., Mabee, W., Pye, K., Xiao, Z.Z., Zhang, X., Saddler, J., Biorefining of softwoods using ethanol organosolv pulping: preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products. Biotechnol. Bioeng. 90:4 (2005), 473–481.
35. [35] Pan, X.J., Gilkes, N., Kadla, J., Pye, K., Saka, S., Gregg, D., Ehara, K., Xie, D., Lam, D., Saddler, J., Bioconversion of hybrid poplar to ethanol and co-products using an organosolv fractionation process: optimization of process yields. Biotechnol. Bioeng. 94:5 (2006), 851–861.
36. [36] Rollin, J.A., Zhu, Z., Sathitsuksanoh, N., Zhang, Y.H., Increasing cellulose accessibility is more important than removing lignin: a comparison of cellulose solvent-based lignocellulose fractionation and soaking in aqueous ammonia. Biotechnol. Bioeng. 108:1 (2011), 22–30.
37. [37] Pan, X.J., Role of functional groups in lignin inhibition of enzymatic hydrolysis of cellulose to glucose. J. Biobased Mater Bioenergy 2:1 (2008), 25–32.
38. [38] Qin, C., Clarke, K., Li, K., Interactive forces between lignin and cellulase as determined by atomic force microscopy. Biotechnol. Biofuels, 7, 2014, 65.
39. [39] Ko, J.K., Um, Y., Park, Y.C., Seo, J.H., Kim, K.H., Compounds inhibiting the bioconversion of hydrothermally pretreated lignocellulose. Appl. Microbiol. Biotechnol. 99:10 (2015), 4201–4212.
40. [40] Kim, Y., Ximenes, E., Mosier, N.S., Ladisch, M.R., Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass. Enzym Microb. Technol. 48 (2011), 408–415.
41. [41] Ximenes, E., Kim, Y., Mosier, N., Dien, B., Ladisch, M., Deactivation of cellulases by phenols. Enzym Microb. Technol. 48 (2011), 54–60.
42. [42] Liu, H., Zhu, J.Y., Fu, S.Y., Effects of lignin-metal complexation on enzymatic hydrolysis of cellulose. J. Agric. Food Chem. 58 (2010), 7233–7238.
43. [43] Eriksson, T., Börjesson, J., Tjerneld, F., Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose. Enzyme Microb. Technol. 31 (2002), 353–364.
44. [44] Nakagame, S., Chandra, R.P., Kadla, J.F., Saddler, J.N., The isolation, characterization and effect of lignin isolated from steam pretreated Douglas-fir on the enzymatic hydrolysis of cellulose. Bioresour. Technol. 102 (2011), 4507–4517.
45. [45] Berlin, A., Balakshin, M., Gilkes, N., Kadla, J., Maximenko, V., Kubo, S., Saddler, J., Inhibition of cellulase, xylanase and beta-glucosidase activities by softwood lignin preparations. J. Biotechnol. 125:2 (2006), 198–209.
46. [46] Zhao, X., Zhang, L., Liu, D., Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose. Biofuels Bioprod. Biorefin 6:4 (2012), 465–482.
47. [47] Norde, W., Adsorption of proteins from solution at the solid-liquid interface. Adv. Colloid Interface Sci. 25:4 (1986), 267–340.
48. [48] Hansen, J., Ely, K., Horsley, D., Herron, J., Hlady, V., Andrade, J.D., The adsorption of lysozymes: a model system. Makromol. Chem. Macromol. Symp. 17 (1988), 135–154.
49. [49] Palonen, H., Tjerneld, F., Zacchi, G., Tenkanen, M., Adsorption of Trichoderma reesei CBH I and EG II and their catalytic domains on steam pretreated softwood and isolated lignin. J. Biotechnol. 107:1 (2004), 65–72.
50. [50] Costaouëc, T.L., Pakarinen, A., Várnai, A., Puranen, T., Viikari, L., The role of carbohydrate binding module (CBM) at high substrate consistency: comparison of Trichoderma reesei and Thermoascus aurantiacus Cel7A (CBHI) and Cel5A (EGII). Bioresour. Technol. 143 (2013), 196–203.
51. [51] Yemshanov, D., McKenney, D.W., Fast-growing poplar plantations as a bioenergy 52 source for Canada. Biomass Bioenergy 32 (2008), 185–197.
52. [52] Borjesson, J., Peterson, R., Tjerneld, F., Enhanced enzymatic conversion of softwood lignocellulose by poly(ethylene glycol) addition. Enzyme Microb. Technol. 40 (2007), 754–762.
53. [53] Borjesson, J., Engqvist, M., Sipos, B., Tjerneld, F., Effect of poly(ethylene glycol) on enzymatic hydrolysis and adsorption of cellulase enzymes to pretreated lignocellulose. Enzyme Microb. Technol. 41:1–2 (2007), 186–195.
54. [54] Hodgson, K.T., Berg, J.C., Dynamic wettability properties of single wood pulp fibers and their relationship to absorbency. Wood Fiber Sci. 20:1 (1988), 3–17.
55. [55] Eriksson, M., Notley, S.M., Wågberg, L., Cellulose thin films: degree of cellulose ordering and its influence on adhesion. Biomacromolecules 8:3 (2007), 912–919.
56. [56] Gunnars, S., Wagberg, L., Stuart, M.A.C., Model films of cellulose: I. Method development and initial results. Cellulose 9:3−4 (2002), 239–249.
57. [57] Notley, S.M., Norgren, M., Surface energy and wettability of spin-coated thin films of lignin isolated from wood. Langmuir 26:8 (2010), 5484–5490.
58. [58] Norgren, M., Gardlund, L., Notley, S.M., Htun, M., Wagberg, L., Smooth model surfaces from lignin derivatives. II. Adsorption of polyelectrolytes and PECs monitored by QCM-D. Langmuir 23:7 (2007), 3737–3743.
59. [59] Norgren, M., Notley, S.M., Majtnerova, A., Gellerstedt, G., Smooth model surfaces from lignin derivatives. I. Preparation and characterization. Langmuir 22:3 (2006), 1209–1214.
60. [60] Nakagame, S., Chandra, R.P., Saddler, J.N., The effect of isolated lignins, obtained from a range of pretreated lignocellulosic substrates, on enzymatic hydrolysis. Biotechnol. Bioeng. 105:5 (2010), 871–879.
61. [61] Sewalt, V.J.H., Glasser, W.G., Beauchemi, K.A., Lignin impact on fiber degradation. 3. Reversal of inhibition of enzymatic hydrolysis by chemical modification of lignin and by additives. J. Agric. Food Chem. 45:5 (1997), 1823–1828.
62. [62] Sewalt, V.J.H., Olivera, W.D., Glasser, W.G., Fontenot, J.P., Lignin impact on fiber degradation. 2. A model study using cellulose hydrogels. J. Sci. Food Agric. 71 (1996), 204–208.
63. [63] Nakagame, S., Chandra, R.P., Kadla, J.F., Saddler, J.N., Enhancing the enzymatic hydrolysis of lignocellulosic biomass by increasing the carboxylic acid content of the associated lignin. Biotechnol. Bioeng. 108 (2011), 538–548.
64. [64] Elgersma, A.V., Zsom, R.L.J., Norde, W., Lyklema, J., The adsorption of bovine serum-albumin on positively and negatively charged polystyrene lattices. J. Colloid Interface Sci. 138:1 (1990), 145–156.
65. [65] Ragnar, M., Lindgren, C.T., Nilvebran, N., pKa-values of guaiacyl and syringyl phenols related to lignin. J. Wood Chem. Technol. 20 (2000), 277–305.
66. [66] Lou, H., Zhu, J.Y., Lan, T.Q., Lai, H., Qiu, X., pH-induced lignin surface modification to reduce nonspecific cellulase binding and enhance enzymatic saccharification of lignocelluloses. ChemSusChem 6 (2013), 919–927.
67. [67] Lan, T.Q., Lou, H., Zhu, J.Y., Enzymatic saccharification of lignocelluloses should be conducted at elevated pH 5.2–6.2. Bioenergy Res. 6:2 (2013), 476–485.
68. [68] McCleary, B.V., Harrington, J., Purification of beta-D-glucosidase from Aspergillus niger. Methods Enzymol. 160 (1988), 575–583.
69. [69] Yang, B., Wyman, C.E., BSA treatment to enhance enzymatic hydrolysis of cellulose in lignin containing substrates. Biotechnol. Bioeng. 94:4 (2006), 611–617.
70. [70] Bhardwaj, N.K., Kumar, S., Bajpai, P.K., Effects of processing on zeta potential and cationic demand of kraft pulps. Colloids Surf. A 246:1−3 (2004), 121–125.
71. [71] Liu, H., Zhu, J.Y., Eliminating inhibition of enzymatic hydrolysis by lignosulfonate in unwashed sulfite-pretreated aspen using metal salts. Bioresour. Technol. 101:23 (2010), 9120–9127.
72. [72] Liu, H., Zhu, J.Y., Fu, S.Y., Effects of lignin-metal complexation on enzymatic hydrolysis of cellulose. J. Agric. Food Chem. 58:12 (2010), 7233–7238.
73. [73] Fukuzaki, S., Urano, H., Nagata, K., Adsorption of bovine serum albumin onto metal oxide surfaces. J. Ferment Bioeng. 81:2 (1996), 163–167.
74. [74] L. Cascao-Pereira, T. Kaper, B.R. Kelemen, A. Liu, Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials. Application no. WO/2009/149202. Publication date 10/12/2009.
75. [75] J. Lavigne, B. Scott, M. Whissel, J. Tomashek, Family 6 cellulase with decreased inactivation by lignin. Application no. WO/2010/012102. Publication date 04/02/2010.
76. [76] B.R. Scott, P. StPierre, J. Lavigne, N. Masri, T.C. White, J.J. Tomashek, Novel lignin-resistant cellulase enzymes. Application no. WO2010096931. Publication date 02/09/2010.
77. [77] J.J. Tomashek, B.R. Scott, D. Kolczynski, Carbohydrate binding modules with reduced binding to lignin. Application no. WO/2011/097713. Publication date 18/08/2011.
78. [78] Rahikainen, J.L., Martin-Sampedro, R., Heikkinen, H., Rovio, S., Marjamaa, K., Tamminen, T., Rojas, O.J., Kruus, K., Inhibitory effect of lignin during cellulose bioconversion: the effect of lignin chemistry on non-productive enzyme adsorption. Bioresour. Technol. 133 (2013), 270–278.
79. [79] McCann, M.C., Carpita, N.C., Designing the deconstruction of plant cell walls. Curr. Opin. Plant Biol. 11:3 (2008), 314–320.
80. [80] Ding, S.Y., Himmel, M.E., The maize primary cell wall microfibril: a new model derived from direct visualization. J. Agric. Food Chem. 54:3 (2006), 597–606.
81. [81] Cosgrove, D.J., Growth of the plant cell wall. Nat. Rev. Mol. Cell Biol. 6:11 (2005), 850–861.
82. [82] Nakagame, S., Chandra, R.P., Saddler, J.N., The influence of lignin on the enzymatic hydrolysis of pretreated biomass substrates. Zhu, J.Y., Zhang, X., Pan, X., (eds.) Sustainable Production of Fuels, Chemicals, and Fibers from Forest Biomass, ACS Symposium Series, Washington DC, USA, 2011, 145–167.
83. [83] Kumar, L., Arantes, V., Chandra, R., Saddler, J., The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility. Bioresour. Technol. 103 (2012), 201–208.
84. [84] Mooney, C.A., Mansfield, S.D., Touhy, M.G., Saddler, J.N., The effect of initial pore volume and lignin content on the enzymatic hydrolysis of softwoods. Bioresour. Technol. 64 (1998), 113–119.
85. [85] Varnai, A., Siika-aho, M., Viikari, L., Restriction of enzymatic hydrolysis of pretreated spruce by lignin. Enzyme Microb. Technol. 46 (2010), 185–193.
86. [86] Chapple, C., Ladisch, M., Meilan, R., Loosening lignin's grip on biofuel production. Nat. Biotechnol. 25:7 (2007), 746–748.
87. [87] Kim, Y., Ximenes, E., Mosier, N.S., Ladisch, M.R., Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass. Enzyme Microb. Technol. 48:4–5 (2011), 408–415.
88. [88] Pan, X.J., Zhang, X., Gregg, D.J., Saddler, J.N., Enhanced enzymatic hydrolysis of steam-exploded Douglas fir by alkaline oxygen post-treatment. Appl. Biochem. Biotechnol. 115 (2004), 1103–1114.
89. [89] Yang, B., Wyman, C.E., Effect of xylan and lignin removal by batch and flow through pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnol. Bioeng. 86:1 (2004), 88–95.
90. [90] Donohoe, B.S., Decker, S.R., Tucker, M.P., Himmel, M.E., Vinzant, T.B., Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment. Biotechnol. Bioeng. 101:5 (2008), 913–925.
91. [91] Li, J.B., Henriksson, G., Gellerstedt, G., Lignin depolymerization/repolymerization and its critical role for delignification of aspen wood steam expolsion. Bioresour. Technol. 98 (2007), 3061–3068.
92. [92] Pu, Y., Hu, F., Huang, F., Davison, B.H., Ragauskas, A.J., Assessing the molecular structure basis for biomass recalcitrance during dilute acid and hydrothermal pretreatments. Biotechnol. Biofuels, 6, 2013, 15.
93. [93] Cannella, D., Hsieh, C.W., Felby, C., Jørgensen, H., Production and effect of aldonic acids during enzymatic hydrolysis of lignocellulose at high dry matter content. Biotechnol. Biofuels, 5, 2012, 26.
94. [94] Pan, X., Yang, Q., Toward a Fundamental Understanding of How Lignin Impacts Enzymatic Saccharification of Lignocelluloses. 247th ACS National Meeting & Exposition. March 16-20, 2014, United States, Dallas, TX CELL-116.
95. [95] Yang, Q., Pan, X., Correlation between lignin physicochemical properties and inhibition to enzymatic hydrolysis of cellulose. Biotechnol. Bioeng., 2016, 10.1002/bit.25903.
96. [96] Shuai, L., Yang, Q., Zhu, J.Y., Lu, F.C., Weimer, P.J., Ralph, J., Pan, X.J., Comparative study of SPORL and dilute-acid pretreatments of spruce for cellulosic ethanol production. Bioresour. Technol. 101:9 (2010), 3106–3114.
97. [97] Kawamoto, H., Nakatsubo, F., Murakami, K., Protein-adsorbing capacities of lignin samples. Mokuzai Gakkaishi 38 (1992), 81–84.
98. [98] Ko, J.K., Ximenes, E., Kim, Y., Ladisch, M.R., Adsorption of enzyme onto lignins of liquid hot water pretreated hardwoods. Biotechnol. Bioeng. 112 (2015), 447–456.
99. [99] Rahikainen, J., Mikander, S., Marjamaa, K., Tamminen, T., Lappas, A., Viikari, L., Kruus, K., Inhibition of enzymatic hydrolysis by residual lignins from softwood–study of enzyme binding and inactivation on lignin-rich surface. Biotechnol. Bioeng. 108 (2011), 2823–2834.
100. [100] Berlin, A., Balakshin, M., Gilkes, N., Kadla, J., Maximenko, V., Kubo, S., Saddler, J., Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations. J. Biotechnol. 125 (2006), 198–209.
101. [101] Sipos, B., Dienes, D., Schleicher, Á., Perazzini, R., Crestini, C., Siika-aho, M., Réczey, K., Hydrolysis efficiency and enzyme adsorption on steam-pretreated spruce in the presence of poly(ethylene glycol). Enzym Microb. Technol. 47 (2010), 84–90.
102. [102] Nordwald, E.M., Brunecky, R., Himmel, M.E., Beckham, G.T., Kaar, J.L., Charge engineering of cellulases improves ionic liquid tolerance and reduces lignin inhibition. Biotechnol. Bioeng. 111 (2014), 1541–1549.
103. [103] Payne, C.M., Knott, B.C., Mayes, H.B., Hansson, H., Himmel, M.E., Sandgren, M., Ståhlberg, J., Beckham, G.T., Fungal cellulases. Chem. Rev. 115 (2015), 1308–1448.
104. [104] Boraston, A.B., Bolam, D.N., Gilbert, H.J., Davies, G.J., Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem. J. 382 (2004), 769–781.
105. [105] Karlsson, J., Siika-aho, M., Tenkanen, M., Tjerneld, F., Enzymatic properties of the low molecular mass endoglucanases Cel12A (EG III) and Cel45A (EG V) of Trichoderma reesei. J Biotechnol 99 (2002), 63–78.
106. [106] Divne, C., Stahlberg, J., Reinikainen, T., Ruohonen, L., Pettersson, G., Knowles, J.K., Teeri, T.T., Jones, T.A., The three-dimensional crystal structure of the catalytic core of cellobiohydrolase I from Trichoderma reesei. Science 265 (1994), 524–528.
107. [107] Davies, G., Henrissat, B., Structures and mechanisms of glycosyl hydrolases. Structure 3 (1995), 853–859.
108. [108] Vaaje-Kolstad, G., Westereng, B., Horn, S.J., Liu, Z., Zhai, H., Sørlie, M., Eijsink, V.G.H., An oxidative enzyme boosting the enzymatic conversion of recalcitrant polysaccharides. Science 330 (2010), 219–222.
109. [109] Linder, M., Mattinen, M.L., Kontteli, M., Lindeberg, G., Ståhlberg, J., Drakenberg, T., Reinikainen, T., Pettersson, G., Annila, A., Identification of functionally important amino acids in the cellulose-binding domain of Trichoderma reesei cellobiohydrolase I. Protein Sci. 4 (1995), 1056–1064.
110. [110] Pfeiffer, K.A., Sorek, H., Roche, C., Strobel, K., Blanch, H.W., Clark, D.S., Evaluating endoglucanase Cel7B-lignin interaction mechanisms and kinetics using quartz crystal microgravimetry. Biotechnol. Bioeng. 212:11 (2015), 2255–2266.
111. [111] Varnai, A., Siika-aho, M., Viikari, L., Carbohydrate-binding modules (CBMs) revisited. Reduced amount of water counterbalances the need for CBMs. Biotechnol. Biofuels, 6, 2013, 30.
112. [112] Pakarinen, A., Ø, Haven M., Djajadi, D.T., Varnai, A., Puranen, T., Viikari, L., Cellulases without carbohydrate binding modules in high consistency ethanol production process. Biotechnol. Biofuels, 7, 2014, 27.
113. [113] Berlin, A., Gilkes, N., Kurabi, A., Bura, R., Tu, M.B., Kilburn, D., Saddler, J., Weak lignin-binding enzymes-a novel approach to improve activity of cellulases for hydrolysis of lignocellulosics. Appl. Biochem. Biotechnol. 121 (2005), 163–170.
114. [114] Park, J.W., Park, K., Song, H., Shin, H., Saccharification and adsorption characteristics of modified cellulases with hydrophilic/hydrophobic copolymers. J. Biotechnol. 93:3 (2002), 203–208.
115. [115] Eriksson, T., Börjesson, J., Tjerneld, F., Mechanism of surfactant effect in enzymatic hydrolysis of lignocelluloses. Enzym Microb. Technol. 31 (2002), 353–364.
116. [116] Kumar, L., Arantes, V., Chandra, R., Saddler, J., The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility. Bioresour. Technol. 103 (2012), 201–208.
117. [117] Yang, B., Wyman, C.E., BSA treatment to enhance enzymatic hydrolysis of cellulose in lignin containing substrates. Biotechnol. Bioeng. 94 (2006), 611–617.
118. [118] Kim, Y., Kreke, T., Ko, J.K., Ladisch, M.R., Hydrolysis-determining substrate characteristics in liquid hot water pretreated hardwood. Biotechnol. Bioeng. 112 (2015), 677–687.
119. [119] Han, Y., Chen, H., Synergism between hydrophobic proteins of corn stover and cellulase in lignocellulose hydrolysis. Biochem. Eng. J. 48 (2010), 218–224.
120. [120] B. Yang, C.E. Wyman. Lignin blockers and uses thereof. 2013; US Patent 8,580,541.
121. [121] Kim, I.J., Ko, H.J., Kim, T.W., Nam, K.H., Choi, I.G., Kim, K.H., Binding characteristics of a bacterial expansin (BsEXLX1) for various types of pretreated lignocellulose. Appl. Microbiol. Biotechnol. 97 (2013), 5381–5388.
122. [122] Lin, X.L., Qiu, X.Q., Yuan, L., Li, Z.H., Lou, H.M., Zhou, M.S., Yang, D.J., Lignin-based polyoxyethylene ether enhanced enzymatic hydrolysis of lignocelluloses by dispersing cellulase aggregates. Bioresour. Technol. 185 (2015), 165–170.
123. [123] Lin, X.L., Qiu, X.Q., Zhu, D.M., Li, Z.H., Zhan, N.X., Zheng, J.Y., Lou, H.M., Zhou, M.S., Yang, D.J., Effect of the molecular structure of lignin-based polyoxyethylene ether on enzymatic hydrolysis efficiency and kinetics of lignocelluloses. Bioresour. Technol. 193 (2015), 266–273.
124. [124] Zheng, Y., Pan, Z., Zhang, R., Wang, D., Jenkins, B., Non-ionic surfactants and non-catalytic protein treatment on enzymatic hydrolysis of pretreated creeping wild ryegrass. Appl Biochem. Biotechnol. 146 (2008), 231–248.
125. [125] Lin, X.L., Qiu, X.Q., Lou, H.M., Li, Z.H., Zhan, N.G., Huang, J.H., Pang, Y.X., Enhancement of lignosulfonate-based polyoxyethylene ether on enzymatic hydrolysis of lignocelluloses. Ind. Crops Prod. 80 (2016), 86–92.
126. [126] Várnai, A., Mäkelä, M.R., Djajadi, D.T., Rahikainen, J., Hatakka, A., Viikari, L., Carbohydrate-binding modules of fungal cellulases: occurrence in nature, function, and relevance in industrial biomass conversion. Adv. Appl. Microbiol. 88 (2014), 103–165.
127. [127] Sukumaran, R.K., Singhania, R.R., Mathew, G.M., Pandey, A., Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bioethanol production. Renew. Energy 34:2 (2009), 421–424.
128. [128] Meng, X., Ragauskas, A.J., Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates. Curr. Opin. Biotechnol. 27 (2014), 150–158.
129. [129] Li, C., Cheng, G., Balan, V., Kent, M.S., Ong, M., Chundawat, S.P.S., Ld, Sousa, Melnichenko, Y.B., Dale, B.E., Simmons, B.A., Singh, S., Influence of physico-chemical changes on enzymatic digestibility of ionic liquid and AFEX pretreated corn stover. Bioresour. Technol. 102 (2011), 6928–6936.
130. [130] Wiman, M., Dienes, D., Hansen, M.A., van der, M.T., Zacchi, G., Liden, G., Cellulose accessibility determines the rate of enzymatic hydrolysis of steam-pretreated spruce. Bioresour. Technol. 126 (2012), 208–215.
131. [131] Rouquerol, J., Baron, G., Denoyel, R., Giesche, H., Groen, J., Klobes, P., Levitz, P., Neimark, A.V., Rigby, S., Skudas, R., et al. Liquid intrusion and alternative methods for the characterization of macroporous materials (IUPAC technical report). Pure Appl. Chem. 84 (2012), 107–136.
132. [132] Giesche, H., Mercury porosimetry: a general (practical) overview. Part Part Syst. Charact. 23 (2006), 9–19.
133. [133] Grethlein, H.E., The effect of pore size distribution on the rate of enzymic hydrolysis of cellulosic substrates. Biotechnology 3 (1985), 155–160.
134. [134] Thompson, D.N., Chen, H.C., Grethlein, H.E., Comparison of pretreatment methods on the basis of available surface area. Bioresour. Technol. 39 (1992), 155–163.
135. [135] Arantes, V., Saddler, J.N., Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates. Biotechnol. Biofuels, 4, 2011, 3.
136. [136] Keshwani, D.R., Cheng, J.J., Microwave-based alkali pretreatment of switchgrass and coastal bermuda grass for bioethanol production. Biotechnol. Prog. 26 (2010), 644–652.
137. [137] Wang, Q.Q., He, Z., Zhu, Z., Zhang, Y.H.P., Ni, Y., Luo, X.L., Zhu, J.Y., Evaluations of cellulose accessibilities of lignocelluloses by solute exclusion and protein adsorption techniques. Biotechnol. Bioeng. 109 (2012), 381–389.
138. [138] Lupoi, J.S., Singh, S., Parthasarathi, R., Simmons Blake, A., Henry Robert, J., Recent innovations in analytical methods for the qualitative and quantitative assessment of lignin. Renew. Sustain. Energy Rev. 49 (2015), 871–906.
139. [139] Mok, Y.K., The Role of Adsorbed Enzymes in Determining the Hydrolysis Kinetics of pretreated lignocellulosic biomass. (Ph.D. thesis), 2015 available from: https://open.library.ubc.ca/collections/24/items/1.0167205/source (accessed on 31 12 15.).
140. 4 coupled ninhydrin-based assay for the quantification of protein/enzymes during the enzymatic hydrolysis of pretreated lignocellulosic biomass. Appl. Biochem. Biotechnol. 176:6 (2015), 1564–1580.