A NaBH4 Coupled Ninhydrin-Based Assay for the Quantification of Protein/Enzymes During the Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass

Applied Biochemistry and Biotechnology

Volumn 176, Issue 6, 2015, Pages 1564-1580

  Mok, Yiu Ki ^a   Arantes, Valdeir ^b   Saddler, Jack N ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Cellulase enzymes; Enzymatic hydrolysis; Lignocellulose; Ninhydrin; Protein quantification

Indexed keywords

ASSAYS; BIOMASS; ENZYME KINETICS; SODIUM BOROHYDRIDE;

CELLULASE ENZYME; ENZYMATIC HYDROLYSIS CONDITIONS; LIGNOCELLULOSE; LIGNOCELLULOSIC BIOMASS; NINHYDRIN; PROTEIN DETERMINATION; PROTEIN QUANTIFICATION; TECHNO-ECONOMIC ASSESSMENT;

ENZYMATIC HYDROLYSIS;

ACETIC ACID; ARABINOSE; CELLULASE; ENZYME; GALACTOSE; GLUCOSE; HEMICELLULOSE; LIGNOCELLULOSE; MANNOSE; MONOSACCHARIDE; NINHYDRIN; OLIGOSACCHARIDE; PROTEIN; SODIUM BOROHYDRIDE; XYLOSE; BORANE DERIVATIVE; LIGNIN;

ARTICLE; BIOMASS; CHEMICAL MODIFICATION; COLORIMETRY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME KINETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN HYDROLYSIS; CHEMISTRY; HEAT;

BIOMASS; BOROHYDRIDES; CELLULASES; HOT TEMPERATURE; LIGNIN; NINHYDRIN;

EID: 84937969878     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-015-1662-7     Document Type: Article

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