Detection, phenotyping, and quantification of antigenspecific T cells using a peptide-MHC dodecamer

Proceedings of the National Academy of Sciences of the United States of America

Volumn 113, Issue 13, 2016, Pages E1890-E1897

  Huang, Jun ^d   Zeng, Xun ^a   Sigal, Natalia ^e   Lund, Peder J ^a   Su, Laura F ^b   Huang, Huang ^a   Chien, Yueh Hsiu ^a   Davis, Mark M ^a,c  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c STANFORD UNIVERSITY   (United States)

^d UNIVERSITY OF CHICAGO   (United States)

^e FLUIDIGM CORPORATION   (United States)

Author keywords

Antigen specific T cell; Avidity; Dodecamer; Peptide MHC

Indexed keywords

CYTOKINE; MAJOR HISTOCOMPATIBILITY ANTIGEN; PEPTIDE; SCAFFOLD PROTEIN; STREPTAVIDIN; T LYMPHOCYTE RECEPTOR; TETRAMER; LYMPHOCYTE ANTIGEN RECEPTOR;

ANIMAL CELL; ANTIGEN SPECIFICITY; ARTICLE; BINDING AFFINITY; BIOTINYLATION; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL COMPARTMENTALIZATION; CELL ISOLATION; CONTROLLED STUDY; EFFECTOR CELL; FLOW CYTOMETRY; GAMMA DELTA T LYMPHOCYTE; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULE; NEXT GENERATION SEQUENCING; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN FOLDING; PROTEIN STRUCTURE; T LYMPHOCYTE; THYMOCYTE; TIME OF FLIGHT MASS SPECTROMETRY; ANIMAL; CHEMISTRY; GENETICS; IMMUNOLOGY; IMMUNOPHENOTYPING; MAJOR HISTOCOMPATIBILITY COMPLEX; METABOLISM; PARASITOLOGY; PHYSIOLOGY; PROCEDURES; SINGLE CELL ANALYSIS; TRANSGENIC MOUSE;

ANIMALS; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; FLOW CYTOMETRY; HUMANS; IMMUNOPHENOTYPING; MAJOR HISTOCOMPATIBILITY COMPLEX; MICE, TRANSGENIC; PEPTIDES; RECEPTORS, ANTIGEN, T-CELL; SINGLE-CELL ANALYSIS; T-LYMPHOCYTES;

EID: 84961990511     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1602488113     Document Type: Article

References (35)

1. Davis MM, et al. (1998) Ligand recognition by alpha beta T cell receptors. Annu Rev Immunol 16:523-544.
2. Altman JD, et al. (1996) Phenotypic analysis of antigen-specific T lymphocytes. Science 274(5284):94-96.
3. Toebes M, et al. (2006) Design and use of conditional MHC class I ligands. Nat Med 12(2):246-251.
4. Hadrup SR, et al. (2009) Parallel detection of antigen-specific T-cell responses by multidimensional encoding of MHC multimers. Nat Methods 6(7):520-526.
5. Newell EW, Klein LO, Yu W, Davis MM (2009) Simultaneous detection of many T-cell specificities using combinatorial tetramer staining. Nat Methods 6(7):497-499.
6. Newell EW, et al. (2013) Combinatorial tetramer staining and mass cytometry analysis facilitate T-cell epitope mapping and characterization. Nat Biotechnol 31(7):623-629.
7. Moon JJ, et al. (2007) Naive CD4(+) T cell frequency varies for different epitopes and predicts repertoire diversity and response magnitude. Immunity 27(2):203-213.
8. Dolton G, et al. (2014) Comparison of peptide-major histocompatibility complex tetramers and dextramers for the identification of antigen-specific T cells. Clin Exp Immunol 177(1):47-63.
9. Mallet-Designe VI, et al. (2003) Detection of low-avidity CD4+ T cells using recombinant artificial APC: Following the antiovalbumin immune response. J Immunol 170(1): 123-131.
10. Guillaume P, et al. (2003) Soluble major histocompatibility complex-peptide octamers with impaired CD8 binding selectively induce Fas-dependent apoptosis. J Biol Chem 278(7):4500-4509.
11. Batard P, et al. (2006) Dextramers: New generation of fluorescent MHC class I/peptide multimers for visualization of antigen-specific CD8+ T cells. J Immunol Methods 310(1-2): 136-148.
12. Chattopadhyay PK, et al. (2006) Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry. Nat Med 12(8):972-977.
13. Davis MM, Altman JD, Newell EW(2011) Interrogating the repertoire: Broadening the scope of peptide-MHC multimer analysis. Nat Rev Immunol 11(8):551-558.
14. Wooldridge L, et al. (2009) Tricks with tetramers: How to get the most from multimeric peptide-MHC. Immunology 126(2):147-164.
15. Sabatino JJ Jr, Huang J, Zhu C, Evavold BD (2011) High prevalence of low affinity peptide-MHC II tetramer-negative effectors during polyclonal CD4+ T cell responses. J Exp Med 208(1):81-90.
16. Schild H, et al. (1994) The nature of major histocompatibility complex recognition by gamma delta T cells. Cell 76(1):29-37.
17. Newell EW, Sigal N, Bendall SC, Nolan GP, Davis MM (2012) Cytometry by time-offlight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes. Immunity 36(1):142-152.
18. Howarth M, et al. (2006) A monovalent streptavidin with a single femtomolar biotin binding site. Nat Methods 3(4):267-273.
19. Newell EW, et al. (2011) Structural basis of specificity and cross-reactivity in T cell receptors specific for cytochrome c-I-E(k). J Immunol 186(10):5823-5832.
20. Whelan JA, et al. (1999) Specificity of CTL interactions with peptide-MHC class I tetrameric complexes is temperature dependent. J Immunol 163(8):4342-4348.
21. Huppa JB, Davis MM (2003) T-cell-antigen recognition and the immunological synapse. Nat Rev Immunol 3(12):973-983.
22. Day CL, et al. (2003) Ex vivo analysis of human memory CD4 T cells specific for hepatitis C virus using MHC class II tetramers. J Clin Invest 112(6):831-842.
23. Janeway CA, Travers P, Walport M, Capra JD (1999) Immunobiology: The Immune System in Health and Disease (Elsevier Science Ltd/Garland Publishing, New York), 4th Ed, pp 241-257.
24. Baldwin KK, Trenchak BP, Altman JD, Davis MM (1999) Negative selection of T cells occurs throughout thymic development. J Immunol 163(2):689-698.
25. Bendall SC, et al. (2011) Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science 332(6030):687-696.
26. Doherty PC (2011) The tetramer transformation. J Immunol 187(1):5-6.
27. Boniface JJ, et al. (1998) Initiation of signal transduction through the T cell receptor requires the multivalent engagement of peptide/MHC ligands [corrected]. Immunity 9(4):459-466.
28. Grotenbreg GM, et al. (2008) Discovery of CD8+ T cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers. Proc Natl Acad Sci USA 105(10):3831-3836.
29. Reijonen H, Kwok WW(2003) Use of HLA class II tetramers in tracking antigen-specific T cells and mapping T-cell epitopes. Methods 29(3):282-288.
30. Tungatt K, et al. (2015) Antibody stabilization of peptide-MHC multimers reveals functional T cells bearing extremely low-affinity TCRs. J Immunol 194(1):463-474.
31. Xiong Y, Kern P, Chang H, Reinherz E (2001) T cell receptor binding to a pMHCII ligand is kinetically distinct from and independent of CD4. J Biol Chem 276(8): 5659-5667.
32. Huppa JB, et al. (2010) TCR-peptide-MHC interactions in situ show accelerated kinetics and increased affinity. Nature 463(7283):963-967.
33. Fairhead M, et al. (2014) SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly. J Am Chem Soc 136(35):12355-12363.
34. Zeng X, et al. (2012) γδ T cells recognize a microbial encoded B cell antigen to initiate a rapid antigen-specific interleukin-17 response. Immunity 37(3):524-534.
35. Huppa JB, Gleimer M, Sumen C, Davis MM (2003) Continuous T cell receptor signaling required for synapse maintenance and full effector potential. Nat Immunol 4(8): 749-755.