Significantly enhanced heme retention ability of myoglobin engineered to mimic the third covalent linkage by nonaxial histidine to heme (vinyl) in Synechocystis hemoglobin

Journal of Biological Chemistry

Volumn 290, Issue 4, 2015, Pages 1979-1993

  Uppal, Sheetal ^a   Salhotra, Shikha ^a   Mukhi, Nitika ^a   Zaidi, Fatima Kamal ^b   Seal, Manas ^c   Dey, Somdatta Ghosh ^c   Bhat, Rajiv ^b   Kundu, Suman ^a  

^a UNIVERSITY OF DELHI   (India)

^b JAWAHARLAL NEHRU UNIVERSITY   (India)

^c DEPARTMENT OF MATERIALS SCIENCE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CHROMATOGRAPHY; COVALENT BONDS; ELECTROSPRAY IONIZATION; HEMOGLOBIN; PROTEINS;

AMINO ACID RESIDUES; CLOSE PROXIMITY; COVALENT LINKAGE; CYANOBACTERIUM SYNECHOCYSTIS; LIGAND BINDING; RETENTION ABILITY; REVERSED PHASE CHROMATOGRAPHY; WILD-TYPE PROTEINS;

PORPHYRINS;

HEME; HEMOGLOBIN; HISTIDINE; IRON; MUTANT PROTEIN; MYOGLOBIN; VINYL DERIVATIVE; RECOMBINANT PROTEIN; TRUNCATED HEMOGLOBIN;

AMINO ACID SUBSTITUTION; ARTICLE; AUTOOXIDATION; CHEMICAL ENGINEERING; CONTROLLED STUDY; COVALENT BOND; DISSOCIATION; ELECTROSPRAY MASS SPECTROMETRY; LIGAND BINDING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR STABILITY; NONHUMAN; PH; REVERSED PHASE LIQUID CHROMATOGRAPHY; STRUCTURAL HOMOLOGY; SYNECHOCYSTIS; THERMOSTABILITY; WILD TYPE; AMINO ACID SEQUENCE; CHEMISTRY; CIRCULAR DICHROISM; DIFFERENTIAL SCANNING CALORIMETRY; ELECTRON SPIN RESONANCE; ESCHERICHIA COLI; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; MUTATION; PROCEDURES; PROTEIN ENGINEERING; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

PHYSETERIDAE; SYNECHOCYSTIS; SYNECHOCYSTIS SP.;

AMINO ACID SEQUENCE; CALORIMETRY, DIFFERENTIAL SCANNING; CIRCULAR DICHROISM; ELECTRON SPIN RESONANCE SPECTROSCOPY; ESCHERICHIA COLI; HEME; HEMOGLOBINS; HISTIDINE; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; MYOGLOBIN; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SYNECHOCYSTIS; TRUNCATED HEMOGLOBINS;

EID: 84961292106     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M114.603225     Document Type: Article

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