Consensus brain-derived protein, extraction protocol for the study of human and murine brain proteome using both 2D-DIGE and mini 2DE immunoblotting

Journal of Visualized Experiments

Volumn , Issue 86, 2014, Pages

  Fernandez Gomez, Francisco Jose ^a   Jumeau, Fanny ^a,b   Derisbourg, Maxime ^a   Burnouf, Sylvie ^a   Tran, Hélène ^a   Eddarkaoui, Sabiha ^a   Obriot, Hélène ^a   Dutoit Lefevre, Virginie ^c   Deramecourt, Vincent ^d   Mitchell, Valérie ^b   Lefranc, Didier ^c   Hamdane, Malika ^a   Blum, David ^a   Buée, Luc ^a   Buée Scherrer, Valérie ^a   Sergeant, Nicolas ^a  

^a JEAN PIERRE AUBERT RESEARCH CENTER   (France)

^b Rouen Normandy University Hospital   (France)

^c Institut Fédératif de Recherche 114   (France)

^d LILLE UNIVERSITY HOSPITAL   (France)

Author keywords

2D DIGE (two dimensional fluorescence difference gel electrophoresis); 2DE; Abbreviations: 2DE (two dimensional gel electrophoresis); AD (Alzheimer s disease); Fluorescence; Human and mice brain tissue; IEF (isoelectrofocusing); Immunoblotting; IPG (Immobilized pH gradients); Issue 86; Mini 2DE (mini 2DE immunoblotting); Neurodegeneration; Neuroscience; Proteomics

Indexed keywords

CARBOCYANINE; FLUORESCENT DYE; NERVE PROTEIN; PROTEOME;

ANIMAL; BRAIN; BRAIN CHEMISTRY; CHEMISTRY; HUMAN; IMMUNOBLOTTING; METABOLISM; MOUSE; PROCEDURES; TWO DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS;

ANIMALS; BRAIN; BRAIN CHEMISTRY; CARBOCYANINES; FLUORESCENT DYES; HUMANS; IMMUNOBLOTTING; MICE; NERVE TISSUE PROTEINS; PROTEOME; TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS;

EID: 84961179176     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51339     Document Type: Article

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