Optimization of protein solubilization for the analysis of the CD14 human monocyte membrane proteome using LC-MS/MS

Journal of Proteomics

Volumn 73, Issue 1, 2009, Pages 112-122

  Ye, Xiaoying ^a   Johann Jr , Donald J ^b   Hakami, Ramin M ^c   Xiao, Zhen ^a   Meng, Zhaojing ^a   Ulrich, Robert G ^d   Issaq, Haleem J ^a   Veenstra, Timothy D ^a   Blonder, Josip ^a  

^a SAIC FREDERICK INC   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c OAK RIDGE ASSOCIATED UNIVERSITIES   (United States)

^d UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

Author keywords

CD14 monocyte; Detergents; LC MS MS; Membrane proteins; Methanol; Solubilization

Indexed keywords

BACTERIORHODOPSIN; BUFFER; CD14 ANTIGEN; DETERGENT; MEMBRANE PROTEIN; METHANOL; PROTEOME;

ARTICLE; HUMAN; HUMAN CELL; MASS SPECTROMETRY; MEMBRANE MODEL; MONOCYTE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN ISOLATION; SOLUBILIZATION;

ANTIGENS, CD14; BACTERIORHODOPSINS; CALIBRATION; CASEINS; CHROMATOGRAPHY, LIQUID; HUMANS; HYDROPHOBICITY; MEMBRANE PROTEINS; MONOCYTES; PROTEIN HYDROLYSATES; PROTEOME; PROTEOMICS; SOLUBILITY; TANDEM MASS SPECTROMETRY;

EID: 70349991237     PISSN: 18743919     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jprot.2009.08.008     Document Type: Article

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