In vivo polyester immobilized sortase for tagless protein purification

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Hay, Iain D ^b,d   Du, Jinping ^a   Reyes, Patricia Rubio ^a   Rehm, Bernd H A ^a,c,d  

^a MASSEY UNIVERSITY   (New Zealand)

^b MONASH UNIVERSITY   (Australia)

^c MACDIARMID INSTITUTE FOR ADVANCED MATERIALS AND NANOTECHNOLOGY   (New Zealand)

^d Polybatics Ltd   (New Zealand)

Author keywords

Polyhydroxyalkanoate; Protein purification; Self cleavage; Sortase

Indexed keywords

BCG VACCINE; CALCIUM CHLORIDE; GREEN FLUORESCENT PROTEIN; MALTOSE BINDING PROTEIN; POLYESTER; POLYHYDROXYALKANOIC ACID; RECOMBINANT PROTEIN; SORTASE; SORTASE A; SYNTHETASE; TRIGLYCINE; UNCLASSIFIED DRUG; ACYLTRANSFERASE; AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; CYSTEINE PROTEINASE; GLYCYL-GLYCYL-GLYCINE; HYBRID PROTEIN; OLIGOPEPTIDE; POLY(3-HYDROXYALKANOIC ACID) SYNTHASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ACTIVATION; ESCHERICHIA COLI; IMMOBILIZATION; IN VIVO STUDY; MOLECULAR RECOGNITION; NONHUMAN; POLYESTER SYNTHASE GENE; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; PROTEIN TARGETING; STAPHYLOCOCCUS AUREUS; BIOSYNTHESIS; CHEMISTRY; DRUG EFFECTS; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; CALCIUM CHLORIDE; CYSTEINE ENDOPEPTIDASES; ENZYME ACTIVATION; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; MALTOSE-BINDING PROTEINS; OLIGOPEPTIDES; POLYESTERS; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCUS AUREUS;

EID: 84961141986     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0385-3     Document Type: Article

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