Production of recombinant adeno-associated virus vectors using suspension HEK293 cells and continuous harvest of vector from the culture media for GMP FIX and FLT1 clinical vector

Molecular Therapy

Volumn 24, Issue 2, 2016, Pages 287-297

  Grieger, Joshua C ^a,b   Soltys, Stephen M ^a   Samulski, Richard Jude ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b Bamboo Therapeutics Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD CLOTTING FACTOR 9; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; PARVOVIRUS VECTOR; PLASMID DNA; POLYETHYLENEIMINE; POLYETHYLENEIMINE MAX; UNCLASSIFIED DRUG; VASCULOTROPIN RECEPTOR 1; FLT1 PROTEIN, HUMAN; SERUM-FREE MEDIUM;

ADENO ASSOCIATED VIRUS 2; ADENO ASSOCIATED VIRUS 8; ADENO ASSOCIATED VIRUS 9; ARTICLE; BIOREACTOR; CELL CLONE; CELL CULTURE; CELL DENSITY; CELL SUSPENSION; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE OPTIMIZATION; GOOD MANUFACTURING PRACTICE; HEK293 CELL LINE; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; SEROTYPE; SILVER STAINING; TRANSIENT TRANSFECTION; TRANSMISSION ELECTRON MICROSCOPY; VIRAL GENE DELIVERY SYSTEM; VIROGENESIS; VIRUS CAPSID; VIRUS GENOME; VIRUS INFECTIVITY; VIRUS PURIFICATION; VIRUS RECOMBINANT; BATCH CELL CULTURE; CELL ADHESION; DEPENDOPARVOVIRUS; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES; SERUM-FREE MEDIUM;

BATCH CELL CULTURE TECHNIQUES; BIOREACTORS; CELL ADHESION; CULTURE MEDIA, SERUM-FREE; DEPENDOVIRUS; GENETIC VECTORS; HEK293 CELLS; HUMANS; VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-1;

EID: 84959530609     PISSN: 15250016     EISSN: 15250024     Source Type: Journal    
DOI: 10.1038/mt.2015.187     Document Type: Article

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