Engineering hybrid exosomes by membrane fusion with liposomes

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Sato, Yuko T ^a   Umezaki, Kaori ^a   Sawada, Shinichi ^a   Mukai, Sada Atsu ^a   Sasaki, Yoshihiro ^a   Harada, Naozumi ^b   Shiku, Hiroshi ^b   Akiyoshi, Kazunari ^a  

^a KYOTO UNIVERSITY   (Japan)

^b MIE UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR 2; FLUORESCENT DYE; LIPOSOME; NANOPARTICLE;

ANIMAL; CHEMISTRY; CONFOCAL MICROSCOPY; EXOSOME; FLOW CYTOMETRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; FREEZING; HELA CELL LINE; HUMAN; IMMUNOLOGY; MEMBRANE FUSION; METABOLISM; MOUSE; RAW 264.7 CELL LINE; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; EXOSOMES; FLOW CYTOMETRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; FREEZING; HELA CELLS; HUMANS; LIPOSOMES; MEMBRANE FUSION; MICE; MICROSCOPY, CONFOCAL; NANOPARTICLES; RAW 264.7 CELLS; RECEPTOR, ERBB-2;

EID: 84959489986     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep21933     Document Type: Article

References (43)

1. Valadi, H., et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat. Cell Biol. 9, 654-659 (2007
2. Properzi, F., Logozzi, M., & Fais, S. Exosomes: the future of biomarkers in medicine. Biomark. Med. 7, 769-778 (2013
3. Jia, S., et al. Emerging technologies in extracellular vesicle-based molecular diagnostics. Expert Rev. Mol. Diagn. 14, 307-321 (2014
4. Mulcahy, L. A., Pink, R. C., & Carter, D. R. F. Routes and mechanisms of extracellular vesicle uptake. J. Extracell. Vesicles 3, 1-14 (2014
5. Parolini, I., et al. Microenvironmental pH is a key factor for exosome traffic in tumor cells. J. Biol. Chem. 284, 34211-34222 (2009
6. Alvarez-Erviti, L., et al. Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat. Biotechnol. 29, 341-345 (2011
7. Johnsen, K. B., et al. A comprehensive overview of exosomes as drug delivery vehicles-endogenous nanocarriers for targeted cancer therapy. Biochim. Biophys. Acta. Rev. Cancer 1846, 75-87 (2014
8. Van der Meel, R. R., Fens, M. H., Vader, P., Van Solinge, W. W., & Eniola-Adefeso, O. Extracellular vesicles as drug delivery systems: lessons from the liposome field. J. Control Release 195, 72-85 (2014
9. Montecalvo, A., et al. Mechanism of transfer of functional microRNAs between mouse dendritic cells via exosomes. Blood 119, 756-766 (2011
10. Kanada, M., et al. Differential fates of biomolecules delivered to target cells via extracellular vesicles. Proc. Natl. Acad. Sci. E1433-E1442 (2015
11. Ohno, S.-I., et al. Systemically injected exosomes targeted to EGFR deliver antitumor microRNA to breast cancer cells. Mol. Ther. 21, 185-191 (2012
12. Takahashi, Y., et al. Visualization and in vivo tracking of the exosomes of murine melanoma B16-BL6 cells in mice after intravenous injection. J. Biotechnol. 165, 77-84 (2013
13. Lee, J., et al Liposome-based engineering of cells to package hydrophobic compounds in membrane vesicles for tumor penetrateon. Nano Lett. 15, 2938-2944 (2015
14. Shtam, T. A., et al. Exosomes are natural carriers of exogenous siRNA to human cells in vitro. Cell Commun. Signal 11, 88 (2013
15. Tian, Y., et al. A doxorubicin delivery platform using engineered natural membrane vesicle exosomes for targeted tumor therapy. Biomaterials 35, 2383-2390 (2014
16. Banizs, A. B., et al. In vitro evaluation of endothelial exosomes as carriers for small interfering ribonucleic acid delivery. Int. J. Nanomed. 9, 4223-4230 (2014
17. Cooper, J. M., et al. Systemic exosomal siRNA delivery reduced alpha-synuclein aggregates in brains of transgenic mice. Mov. Disord. 29, 1476-1485 (2014
18. Wahlgren, J., et al. Plasma exosomes can deliver exogenous short interfering RNA to monocytes and lymphocytes. Nucleic Acids Res. 40, e130 (2012
19. Kooijmans, S. A., et al. Electroporation-induced siRNA precipitation obscures the efficiency of siRNA loading into extracellular vesicles, J. Control. Release 172, 229-238 (2013
20. Abuchowski, A., van Es, T., Palczuk, N. C., & Davis, F. F. Alteration of immunological properties of bovine serum albumin by covalent attachment of polyethylene glycol. J. Biol. Chem. 252, 3578-3581 (1977
21. Blume, G., & Cevc, G. Liposomes for the sustained drug release in vivo. Biochim. Biophys. Acta. 1029, 91-97 (1990
22. Matsuoka, T. Recent advances in the HER2 targeted therapy of gastric cancer, World. J. Clin. Cases, 3, 42-51 (2015
23. Struck, D. K., Hoekstra, D., & Pagano, R. E. Use of resonance energy transfer to monitor membrane fusion. Biochemistry 20, 4093-4099 (1981
24. Düzgünes, N. Fluorescence assays for liposome fusion. Methods Enzymol. 372, 260-274 (2003
25. MacDonald, R. I. Energy transfer measurements of fusion between Sendai virus and vesicles corrected for decreased absorption of acceptor probe. J. Biol. Chem. 262, 10392-10397 (1987
26. Trier, S., Henriksen, J. R., & Andresen, T. L. Membrane fusion of pH-sensitive liposomes-A quantitative study using giant unilamellar vesicles. Soft Matter 7, 9027-9034 (2011
27. Mukai, K., et al. HER2 peptide-specific CD8+ T cells are proportionally detectable long after multiple DNA vaccinations. Gene Ther. 9, 879-888 (2002
28. Nagata, Y., et al. Peptides derived from a wild-Type murine proto-oncogene c-erbB-2/HERZ/neu can induce CTL and tumor suppression in syngeneic hosts. J. Immunol. 159, 1336-1343 (1997
29. Tian, T. Exosome uptake through clathrin-mediated endocytosis and macropinocytosis and mediating miR-21 delivery. J. Biol. Chem. 289, 22258-22267 (2014
30. Pick, U. Liposomes with a large trapping capacity prepared by freezing and thawing of sonicated phospholipid mixtures. Arch. Biochem. Biophys. 212, 186-194 (1981
31. Oku, N., & MacDonald, R. C. Differential effects of alkali metal chlorides on formation of giant liposomes by freezing and thawing and dialysis. Biochemistry 22, 855-863 (1983
32. Macdonald R. I., & Macdonald R. C. Lipid mixing during freezing thawing of liposomal membrane as monitored by fluorescence energy transfer. Biochim. Biophys. Acta. 735, 243-251 (1983
33. Talsma, H. M., Steenbergen, J. V., & Crommelin, D. J. A. The cryopreservation of liposomes: 3. Almost compete retention of a watersoluble marker in small liposomes in a cryoprotectant containing dispersion after a freezing/thawing cycle. Int. J. Pharm. 77, 119-126 (1991
34. Costa, A. P., Xu, X., & Burgess, D. J. Freeze-Anneal-Thaw cycling of unilamellar liposomes: Effect on encapsulation efficiency, Pharm. Res. 31, 97-103 (2014
35. MacDonald, R. C., Jones, F. D., & Qiu, R. Fragmentation into small vesicles of dioleoylphosphatidylcholine bilayers during freezing and thawing. Biochim. Biophys. Acta. Biomembr. 1191, 362-370 (1994
36. Rudolph, A. S., & Crowe, J. H. Membrane stabilization during freezing: the role of two natural cryoprotectants, trehalose and proline. Cryobiology 22, 367-377 (1985
37. Siow, L. F., Rades, T., & Lim, M. H. Characterizing the freezing behavior of liposomes as a tool to understand the cryopreservation procedures. Cryobiology 55, 210-221 (2007
38. Allen, T. M., & Cullis, P. R. Liposomal drug delivery systems: From concept to clinical applications, Adv. Drug. Deliv. Rev. 65, 36-48 (2013
39. Lee, J., & Lentz, B. R. Outer leaflet-packing defects promote poly(ethylene glycol)-mediated fusion of large unilamellar vesicles, Biochemistry 36, 421-431 (1997
40. Moritani, Y., Nomura, S. I. M., Morita, I., & Akiyoshi, K. Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation. FEBS. J. 277, 3343-3352 (2010
41. Mineo, M., et al. Exosomes released by K562 chronic myeloid leukemia cells promote angiogenesis in a Src-dependent fashion. Angiogenesis 15, 33-45 (2012
42. Tian, T., et al. Dynamics of exosome internalization and trafficking. J. Cell. Physiol. 228, 1487-195 (2013
43. Mayer, L. D., Hope M. J., & Cullis P. R. Vesicles of variable sizes produced by a rapid extrusion procedure. Biochim. Biophys. Acta. 858, 161-168 (1986