Solubilization and refolding of inclusion body proteins

Methods in Molecular Biology

Volumn 1258, Issue , 2015, Pages 283-291

  Singh, Anupam ^a   Upadhyay, Vaibhav ^a   Panda, Amulya K ^a  

^a NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

Aggregation; Inclusion bodies; Protein refolding; Pulsatile renaturation; Solubilization

Indexed keywords

BUFFER; CYANIC ACID DERIVATIVE; DISULFIDE; GLUTATHIONE; GLUTATHIONE DISULFIDE; GUANIDINE; INCLUSION BODY PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; UREA;

ALKALINITY; ARTICLE; BACTERIAL CELL; CELL INCLUSION; CELL SUSPENSION; DISULFIDE BOND; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN AGGREGATION; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN REFOLDING; PROTEIN RENATURATION; PROTEIN SECONDARY STRUCTURE; SOLUBILIZATION; ANIMAL; HUMAN; METABOLISM; SOLUBILITY;

ANIMALS; BUFFERS; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; PROTEIN FOLDING; RECOMBINANT PROTEINS; SOLUBILITY;

EID: 84958661323     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2205-5_15     Document Type: Article

References (22)

1. Ventura S, Villaverde A (2006) Protein quality in bacterial inclusion bodies. Trends Biotechnol 24:179–185
2. García-Fruitós E, Sabate R, de Groot NS et al (2011) Biological role of bacterial inclusion bodies: A model for amyloid aggregation. FEBS J 278:2419–2427
3. Khan RH, Rao KB, Eshwari AN et al (1998) Solubilization of recombinant ovine growth hormone with retention of native-like secondary structure and its refolding from the inclusion bodies of Escherichia coli. Biotechnol Prog 14:722–728
4. Clark ED (2001) Protein refolding for industrial processes. Curr Opin Biotechnol 12:202–207
5. Burgess RR (2009) Refolding solubilized inclusion body proteins. Methods Enzymol 463:259–282
6. Stockel J, Doring K, Malotka J et al (1997) Pathway of detergent-mediated and peptide ligand-mediated refolding of heterodimeric class II major histocompatibility complex (MHC) molecules. Eur J Biochem 248:684–691
7. Burgess RR (1996) Purifi cation of overproduced Escherichia coli RNA polymerase sigma factors by solubilizing inclusion bodies and refolding from Sarkosyl. Methods Enzymol 273:145–149
8. Panda AK (2003) Bioprocessing of therapeutic proteins from the inclusion bodies of Escherichia coli. Adv Biochem Eng Biotechnol 85:43–93
9. St John RJ, Carpenter JF, Balny C et al (2001) High pressure refolding of recombinant human growth hormone from insoluble aggregates. Structural transformations, kinetic barriers, and energetics. J Biol Chem 276: 46856–46863
10. Panda AK, Singh SM, Upadhyay AK (2008) Process for obtaining bioactive recombinant protein from inclusion bodies. Patent no. US20100273234 (PCT/IN2008/000297)
11. Singh SM, Sharma A, Upadhyay AK et al (2012) Solubilization of inclusion body proteins using n-propanol and its refolding into bioactive form. Protein Expr Purif 81:75–82
12. Hutchinson MH, Morreale G, Middelberg AP et al (2006) Production of enzymatically active ketosteroid isomerase following insoluble expression in Escherichia coli. Biotechnol Bioeng 95:724–733
13. De Bernardez CE, Schwarz E, Rudolph R (1999) Inhibition of aggregation side reactions during in vitro protein folding. Methods Enzymol 309:217–236
14. Upadhyay AK, Murmu A, Singh A et al (2012) Kinetics of inclusion body formation and its correlation with the characteristics of protein aggregates in Escherichia coli. PLoS One 7:e33951
15. Peternel S, Grdadolnik J, Gaberc-Porekar V et al (2008) Engineering inclusion bodies for non denaturing extraction of functional proteins. Microb Cell Fact 7:34
16. Jevsevar S, Gaberc-Porekar V, Fonda I et al (2005) Production of nonclassical inclusion bodies from which correctly folded protein can be extracted. Biotechnol Prog 21:632–639
17. Singh SM, Eshwari AN, Garg LC et al (2005) Isolation, solubilization, refolding, and chromatographic purification of human growth hormone from inclusion bodies of Escherichia coli cells: a case study. Methods Mol Biol 308:163–176
18. Georgiou G, Valax P (1999) Isolating inclusion bodies from bacteria. Methods Enzymol 309:48–58
19. Bowden GA, Paredes AM, Georgiou G (1991) Structure and morphology of protein inclusion bodies in Escherichia coli. Biotechnology (N Y) 9:725–730
20. Street TO, Courtemanche N, Barrick D (2008) Protein folding and stability using denaturants. Methods Cell Biol 84: 295–325
21. Singh SM, Panda AK (2005) Solubilization and refolding of bacterial inclusion body proteins. J Biosci Bioeng 99:303–310
22. Bondos SE, Bicknell A (2003) Detection and prevention of protein aggregation before, during, and after purification. Anal Biochem 316:223–231