Solubilization of recombinant ovine growth hormone with retention of native-like secondary structure and its refolding from the inclusion bodies of Escherichia coli

Biotechnology Progress

Volumn 14, Issue 5, 1998, Pages 722-728

  Khan, R H ^a   Appa Rao, K B C ^a   Eshwari, A N S ^a   Totey, S M ^a   Panda, A K ^a  

^a NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

CIRCULAR DICHROISM SPECTROSCOPY; FLUORESCENCE; GROWTH HORMONE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; SOLUBILIZATION; WESTERN BLOTTING;

BATCH CELL CULTURE; BIOASSAY; COLUMN CHROMATOGRAPHY; CONFORMATIONS; ESCHERICHIA COLI; FERMENTATION; FLUORESCENCE; HORMONES; IMMUNOLOGY; MOLECULAR STRUCTURE; PURIFICATION; SOLUBILITY;

CIRCULAR DICHROISM; GEL FILTRATION CHROMATOGRAPHY; OVINE GROWTH HORMONES; RADIO RECEPTOR BINDING ASSAY; WESTERN BLOTTING;

GENETIC ENGINEERING;

ANIMALS; BIOTECHNOLOGY; CHROMATOGRAPHY, GEL; ESCHERICHIA COLI; GENE EXPRESSION; GROWTH HORMONE; HYDROGEN-ION CONCENTRATION; INCLUSION BODIES; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SHEEP; SOLUBILITY;

ESCHERICHIA COLI; OVIS;

EID: 0032171073     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp980071q     Document Type: Article

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