Purification and characterization of a fish granzymeA involved in cell-mediated immunity

Developmental and Comparative Immunology

Volumn 60, Issue , 2016, Pages 33-40

  Matsuura, Yuta ^a   Yabu, Takeshi ^a   Shiba, Hajime ^a   Moritomo, Tadaaki ^a   Nakanishi, Teruyuki ^a  

^a NIHON UNIVERSITY   (Japan)

Author keywords

Allogeneic; Cell mediated immunity; Cytotoxicity; Ginbuna crucian carp; Granzyme; Purification; Serine protease

Indexed keywords

3,4 DICHLOROISOCOUMARIN; BENZAMIDINE; COUMARIN DERIVATIVE; GRANZYME A; SEPHAROSE; SERINE PROTEINASE; SERINE PROTEINASE INHIBITOR; FISH PROTEIN; GRANZYME; PROTEINASE INHIBITOR;

ANIMAL CELL; ARTICLE; CARP; CELLULAR IMMUNITY; CHROMATOGRAPHY; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FISH; HYDROLYSIS; IMMUNOSTIMULATION; IN VIVO STUDY; LEUKOCYTE; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; ANIMAL; ANTAGONISTS AND INHIBITORS; CELL LINE; CHEMISTRY; IMMUNOLOGY; ISOLATION AND PURIFICATION; KINETICS; PROTEIN DEGRADATION;

ANIMALS; CARPS; CELL LINE; FISH PROTEINS; GRANZYMES; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; IMMUNITY, CELLULAR; KINETICS; PROTEASE INHIBITORS; PROTEOLYSIS; SUBSTRATE SPECIFICITY;

EID: 84958559473     PISSN: 0145305X     EISSN: 18790089     Source Type: Journal    
DOI: 10.1016/j.dci.2016.02.011     Document Type: Article

References (38)

1. Andrade F., Roy S., Nicholson D., Thornberry N., Rosen A., Casciola-Rosen L. Granzyme B directly and efficiently cleaves several downstream caspase substrates: implications for CTL-induced apoptosis. Immunity 1998, 8:451-460.
2. Buzza M.S., Netzel-Arnett S., Shea-Donohue T., Zhao A.P., Lin C.Y., List K., Szabo R., Fasano A., Bugge T.H., Antalis T.M. Membrane-anchored serine protease matriptase regulates epithelial barrier formation and permeability in the intestine. Proc. Natl. Acad. Sci. U. S. A. 2010, 107:4200-4205.
3. Chowdhury D., Beresford P.J., Zhu P., Zhang D., Sung J.S., Demple B., Perrino F.W., Lieberman J. The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell death. Mol. Cell 2006, 23:133-142.
4. Choy J.C. Granzymes and perforin in solid organ transplant rejection. Cell Death Differ. 2010, 17:567-576.
5. Coughlan A.R., Robertson D.H., Burke R., Beynon R.J., Carter S.D. Isolation and identification of canine matrix metalloproteinase-2 (MMP-2). Vet. J. 1998, 155:231-237.
6. Fischer U., Koppang E.O., Nakanishi T. Teleost T and NK cell immunity. Fish. Shellfish Immunol. 2013, 35:197-206.
7. Garciasanz J.A., Macdonald H.R., Jenne D.E., Tschopp J., Nabholz M. Cell specificity of granzyme gene-expression. J. Immunol. 1990, 145:3111-3118.
8. Griffiths G.M., Namikawa R., Mueller C., Liu C.C., Young J.D.E., Billingham M., Weissman I. Granzyme-a and perforin as markers for rejection in cardiac transplantation. Eur. J. Immunol. 1991, 21:687-692.
9. Harper J.W., Hemmi K., Powers J.C. Reaction of serine proteases with substituted isocoumarins - discovery of 3,4-dichloroisocoumarin, a new general mechanism based serine protease inhibitor. Biochemistry 1985, 24:1831-1841.
10. Hasegawa S., Nakayasu C., Okamoto N., Nakanishi T., Ikeda Y. Fin cell line from isogeneic ginbuna crucian carp. In Vitro Cell. Dev. Biol. Anim. 1997, 33:232-233.
11. Jiang Y., Doolittle R.F. The evolution of vertebrate blood coagulation as viewed from a comparison of puffer fish and sea squirt genomes. Proc. Natl. Acad. Sci. U. S. A. 2003, 100:7527-7532.
12. Kam C.M., Hudig D., Powers J.C. Granzymes (lymphocyte serine proteases): characterization with natural and synthetic substrates and inhibitors. Biochim. Biophys. Acta 2000, 1477:307-323.
13. Kanaoka Y., Takahashi T., Nakayama H., Takada K., Kimura T., Sakakibara S. Organic fluorescence reagent .4. Synthesis of a key fluorogenic amide, "L-Arginine-4-Methylcoumaryl-7-Amide (L-Arg-Mca) and its derivatives - fluorescence assays for trypsin and papain. Chem. Pharm. Bull. 1977, 25:3126-3128.
14. Kato H., Adachi N., Ohno Y., Iwanaga S., Takada K., Sakakibara S. New fluorogenic peptide-substrates for plasmin. J. Biochem. 1980, 88:183-190.
15. Kawabata S.I., Miura T., Morita T., Kato H., Fujikawa K., Iwanaga S., Takada K., Kimura T., Sakakibara S. Highly sensitive peptide-4-methylcoumaryl-7-amide substrates for blood-clotting proteases and trypsin. Eur. J. Biochem. 1988, 172:17-25.
16. Kettner C.A., Shenvi A.B. Inhibition of the serine proteases leukocyte elastase, pancreatic elastase, cathepsin-G, and chymotrypsin by peptide boronic acids. J. Biol. Chem. 1984, 259:5106-5114.
17. Kimura A., Ikeo K., Nonaka M. Evolutionary origin of the vertebrate blood complement and coagulation systems inferred from liver EST analysis of lamprey. Dev. Comp. Immunol. 2009, 33:77-87.
18. Krahenbuhl O., Rey C., Jenne D., Lanzavecchia A., Groscurth P., Carrel S., Tschopp J. Characterization of granzymes A and B isolated from granules of cloned human cytotoxic T lymphocytes. J. Immunol. 1988, 141:3471-3477.
19. Li W.A., Barry Z.T., Cohen J.D., Wilder C.L., Deeds R.J., Keegan P.M., Platt M.O. Detection of femtomole quantities of mature cathepsin K with zymography. Anal. Biochem. 2010, 401:91-98.
20. Lineweaver H., Burk D. The determination of enzyme dissociation constants. J. Am. Chem. Soc. 1934, 56:658-666.
21. Mabee C.L., McGuire M.J., Thiele D.L. Dipeptidyl peptidase I and granzyme A are coordinately expressed during CD8+ T cell development and differentiation. J. Immunol. 1998, 160:5880-5885.
22. Mahrus S., Craik C.S. Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem. Biol. 2005, 12:567-577.
23. Matsuura Y., Yabu T., Shiba H., Moritomo T., Nakanishi T. Identification of a novel fish granzyme involved in cell-mediated immunity. Dev. Comp. Immunol. 2014, 46:499-507.
24. Nakanishi T., Toda H., Shibasaki Y., Somamoto T. Cytotoxic T cells in teleost fish. Dev. Comp. Immunol. 2011, 35:1317-1323.
25. Perona J.J., Craik C.S. Evolutionary divergence of substrate specificity within the chymotrypsin-like serine protease fold. J. Biol. Chem. 1997, 272:29987-29990.
26. Peters P.J., Borst J., Oorschot V., Fukuda M., Krahenbuhl O., Tschopp J., Slot J.W., Geuze H.J. Cytotoxic T lymphocyte granules are secretory lysosomes, containing both perforin and granzymes. J. Exp. Med. 1991, 173:1099-1109.
27. Poe M., Bennett C.D., Biddison W.E., Blake J.T., Norton G.P., Rodkey J.A., Sigal N.H., Turner R.V., Wu J.K., Zweerink H.J. Human cytotoxic lymphocyte tryptase - its purification from granules and the characterization of inhibitor and substrate-specificity. J. Biol. Chem. 1988, 263:13215-13222.
28. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 1985, 150:76-85.
29. Somamoto T., Nakanishi T., Nakao M. Identification of anti-viral cytotoxic effector cells in the ginbuna crucian carp, Carassius auratus langsdorfii. Dev. Comp. Immunol. 2013, 39:370-377.
30. Somamoto T., Nakanishi T., Okamoto N. Role of specific cell-mediated cytotoxicity in protecting fish from viral infections. Virology 2002, 297:120-127.
31. Somamoto T., Yoshiura Y., Sato A., Nakao M., Nakanishi T., Okamoto N., Ototake M. Expression profiles of TCRbeta and CD8alpha mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp. Virology 2006, 348:370-377.
32. Toda H., Araki K., Moritomo T., Nakanishi T. Perforin-dependent cytotoxic mechanism in killing by CD8 positive T cells in ginbuna crucian carp, Carassius auratus langsdorfii. Dev. Comp. Immunol. 2011, 35:88-93.
33. Toda H., Shibasaki Y., Koike T., Ohtani M., Takizawa F., Ototake M., Moritomo T., Nakanishi T. Alloantigen-specific killing is mediated by CD8-positive T cells in fish. Dev. Comp. Immunol. 2009, 33:646-652.
34. Toda H., Yabu T., Shiba H., Moritomo T., Nakanishi T. Evaluating antigen-specific cytotoxicity of CD8+ T cells in fish by granzyme B-like activity. Vet. Immunol. Immunopathol. 2011, 141:168-172.
35. Trapani J.A. Granzymes: a family of lymphocyte granule serine proteases. Genome Biol. 2001, 2.
36. Trapani J.A., Smyth M.J. Functional significance of the perforin/granzyme cell death pathway. Nat. Rev. Immunol. 2002, 2:735-747.
37. Zhang D., Pasternack M.S., Beresford P.J., Wagner L., Greenberg A.H., Lieberman J. Induction of rapid histone degradation by the cytotoxic T lymphocyte protease Granzyme A. J. Biol. Chem. 2001, 276:3683-3690.
38. Zimmerman M., Ashe B., Yurewicz E.C., Patel G. Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substrates. Anal. Biochem. 1977, 78:47-51.