Evaluating antigen-specific cytotoxicity of CD8+ T cells in fish by granzyme B-like activity

Veterinary Immunology and Immunopathology

Volumn 141, Issue 1-2, 2011, Pages 168-172

  Toda, Hideaki ^a   Yabu, Takeshi ^a   Shiba, Hajime ^a   Moritomo, Tadaaki ^a   Nakanishi, Teruyuki ^a  

^a NIHON UNIVERSITY   (Japan)

Author keywords

CTL; Cytotoxicity; Ginbuna crucian carp; Granzyme B

Indexed keywords

3,4 DICHLOROISOCOUMARIN; ACETYLISOLEUCYLGLUTAMYLTHREONYLASPARTYL 4 METHYLCOUMARYL 7 AMIDE; BENZYLOXYCARBONYLISOLEUCYLGLUTAMYLTHREONYLASPARTYL FLUOROMETHYL KETONE; CD8 ANTIGEN; FISH PROTEIN; GRANZYME B; PEPTIDE DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ANTIGEN SPECIFICITY; APOPTOSIS; ARTICLE; CARP; CD8+ T LYMPHOCYTE; CELL SURVIVAL; COCULTURE; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOTOXICITY; ENZYME ACTIVITY; ENZYME SPECIFICITY; NONHUMAN; PHARMACOLOGICAL BLOCKING; PROTEIN FUNCTION; SENSITIZATION;

ANIMALS; CARPS; CD8-POSITIVE T-LYMPHOCYTES; CELL LINE; CYTOTOXICITY TESTS, IMMUNOLOGIC; FLOW CYTOMETRY; GRANZYMES; T-LYMPHOCYTES, CYTOTOXIC;

CARASSIUS; MAMMALIA; VERTEBRATA;

EID: 79953310744     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetimm.2011.02.020     Document Type: Article

References (14)

1. Barry M., Bleackley R. Cytotoxic T lymphocytes: all roads lead to death. Nature Reviews Immunology 2002, 2:401-409.
2. Ewen C., Kane K., Shostak I., Griebel P., Bertram E., Watts T., Bleackley R., McElhaney J. A novel cytotoxicity assay to evaluate antigen-specific CTL responses using a colorimetric substrate for Granzyme B. Journal of Immunological Methods 2003, 276:89-101.
3. Grossman W., Revell P., Lu Z., Johnson H., Bredemeyer A., Ley T. The orphan granzymes of humans and mice. Current Opinion in Immunology 2003, 15:544-552.
4. Huang R., Zhong S., Liu H., Kong R.Q., Wang Y.P., Hu W., Guo Q.L. Identification and characterization of common carp (Cyprinus carpio L.) granzyme A/K, a cytotoxic cell granule-associated serine protease. Fish & Shellfish Immunology 2010, 29:388-398.
5. Nakanishi T., Fischer U., Dijkstra J.M., Hasegawa S., Somamoto T., Okamoto N., Ototake M. Cytotoxic T cell function in fish. Developmental and Comparative Immunology 2002, 26:131-139.
6. Praveen K., Evans D.L., Jaso-Friedmann L. Evidence for the existence of granzyme-like serine proteases in teleost cytotoxic cells. Journal of Molecular Evolution 2004, 58:449-459.
7. Shresta S., MacIvor D., Heusel J., Russell J., Ley T. Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells. Proceedings of the National Academy of Sciences of the United States of America 1995, 92:5679-5683.
8. Smyth M.J., Trapani J.A. Granzymes: exogenous proteinases that induce target cell apoptosis. Immunology Today 1995, 16:202-206.
9. Somamoto T., Yoshiura Y., Sato A., Nakao M., Nakanishi T., Okamoto N., Ototake M. Expression profiles of TCRβ and CD8α mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp. Virology 2006, 348:370-377.
10. Thornberry N.A., Rano T.A., Peterson E.P., Rasper D.M., Timkey T., Garcia-Calvo M., Houtzager V.M., Nordstrom P.A., Roy S., Vaillancourt J.P., Chapman K.T., Nicholson D.W. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. Journal of Biological Chemistry 1997, 272:17907-17911.
11. Toda H., Araki K., Moritomo T., Nakanishi T. Perforin-dependent cytotoxic mechanism in killing by CD8 positive T cells in ginbuna crucian carp, Carassius auratus langsdorfii. Developmental and Comparative Immunology 2011, 35:88-93.
12. Toda H., Shibasaki Y., Koike T., Ohtani M., Takizawa F., Ototake M., Moritomo T., Nakanishi T. Alloantigen-specific killing is mediated by CD8-positive T cells in fish. Developmental and Comparative Immunology 2009, 33:646-652.
13. Wernersson S., Reimer J.M., Poorafshar M., Karlson U., Wermenstam N., Bengten E., Wilson M., Pilstrom L., Hellman L. Granzyme-like sequences in bony fish shed light on the emergence of hematopoietic serine proteases during vertebrate evolution. Developmental and Comparative Immunology 2006, 30:901-918.
14. Zhou H., Stuge T.B., Miller N.W., Bengten E., Naftel J.P., Bernanke J.M., Chinchar V.G., Clem L.W., Wilson M. Heterogeneity of channel catfish CTL with respect to target recognition and cytotoxic mechanisms employed. Journal of Immunology 2001, 167:1325-1332.