Serum-Based Culture Conditions Provoke Gene Expression Variability in Mouse Embryonic Stem Cells as Revealed by Single-Cell Analysis

Cell Reports

Volumn 14, Issue 4, 2016, Pages 956-965

  Guo, Guoji ^a,c   Pinello, Luca ^b   Han, Xiaoping ^a,c   Lai, Shujing ^c   Shen, Li ^c   Lin, Ta Wei ^a   Zou, Keyong ^e   Yuan, Guo Cheng ^b   Orkin, Stuart H ^a,d  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

^c ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

^d HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^e Boston Open Labs   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INHIBITOR OF DIFFERENTIATION 2; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR CDX2; TRANSCRIPTION FACTOR GATA 3; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2; TRANSCRIPTOME; CULTURE MEDIUM;

ANIMAL CELL; ARTICLE; CDX2 GENE; CELL CULTURE; CELL DIFFERENTIATION; CELL HETEROGENEITY; CLU GENE; CONTROLLED STUDY; DNA METHYLATION; EMBRYONIC STEM CELL; FGF4 GENE; GATA3 GENE; GENE; GENE ACTIVATION; GENE CLUSTER; GENE EXPRESSION; GENE FUNCTION; GENE IDENTIFICATION; GENETIC VARIABILITY; ID2 GENE; LAMB1 GENE; MOUSE; NANOG GENE; NONHUMAN; POU5F1 GENE; PRIORITY JOURNAL; RNA SEQUENCE; SERUM; SERUM BASED CULTURE; SIGNAL TRANSDUCTION; SINGLE CELL ANALYSIS; SNAI1 GENE; SOX2 GENE; ANIMAL; CELL CULTURE TECHNIQUE; CELL LINE; CHEMISTRY; CHROMATIN ASSEMBLY AND DISASSEMBLY; CULTURE MEDIUM; CYTOLOGY; METABOLISM; PROCEDURES;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; CHROMATIN ASSEMBLY AND DISASSEMBLY; CULTURE MEDIA; EMBRYONIC STEM CELLS; MICE; SERUM; SINGLE-CELL ANALYSIS; TRANSCRIPTOME;

EID: 84958112644     PISSN: None     EISSN: 22111247     Source Type: Journal    
DOI: 10.1016/j.celrep.2015.12.089     Document Type: Article

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