Identification and Characterization of Botulinum Neurotoxin A Substrate Binding Pockets and Their Re-Engineering for Human SNAP-23

Journal of Molecular Biology

Volumn 428, Issue 2, 2016, Pages 372-384

  Sikorra, Stefan ^a   Litschko, Christa ^a   Müller, Carina ^a   Thiel, Nadine ^a   Galli, Thierry ^b   Eichner, Timo ^c   Binz, Thomas ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b UNIVERSITÉ PARIS DESCARTES   (France)

^c BRANDEIS UNIVERSITY   (United States)

Author keywords

molecular dynamics simulation; screening method; SNAP 25; substrate specificity; zinc protease

Indexed keywords

BOTULINUM TOXIN A; MUTANT PROTEIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 23; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; PROTEIN BINDING; QB SNARE PROTEIN; QC SNARE PROTEIN; SNAP23 PROTEIN, HUMAN;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; CRYSTAL STRUCTURE; HUMAN; IN VITRO STUDY; MOLECULAR DYNAMICS; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; REVERTANT; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; DNA MUTATIONAL ANALYSIS; GENETIC SCREENING; GENETICS; METABOLISM; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SACCHAROMYCES CEREVISIAE;

BINDING SITES; BOTULINUM TOXINS, TYPE A; DNA MUTATIONAL ANALYSIS; GENETIC TESTING; HUMANS; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEOLYSIS; QB-SNARE PROTEINS; QC-SNARE PROTEINS; SACCHAROMYCES CEREVISIAE;

EID: 84957449878     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2015.10.024     Document Type: Article

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