Human whole-blood culture system for ex vivo characterization of designer-cell function

Biotechnology and Bioengineering

Volumn 113, Issue 3, 2016, Pages 588-597

  Schukur, Lina ^a   Geering, Barbara ^a   Fussenegger, Martin ^a,b  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Alginate; Inflammatory disorder; Personalized medicine; Primary cells; Synthetic biology; Tumor necrosis factor

Indexed keywords

ALGINATE; AMINO ACIDS; ANIMAL CELL CULTURE; BIOMATERIALS; BLOOD; CYTOLOGY; DIAGNOSIS; ENCAPSULATION; GENE EXPRESSION; GENE THERAPY; GLYCOPROTEINS; LYMPHOCYTES; MACROPHAGES; MAMMALS; PHOSPHATASES; TUMORS;

INFLAMMATORY DISORDERS; PERSONALIZED MEDICINES; PRIMARY CELLS; SYNTHETIC BIOLOGY; TUMOR NECROSIS FACTORS;

CELL SIGNALING;

ALGINATE(POLY LEVO LYSINE) ALGINATE; ALGINIC ACID; BACTERIUM LIPOPOLYSACCHARIDE; TUMOR NECROSIS FACTOR; UNCLASSIFIED DRUG; BIOMATERIAL; GLUCURONIC ACID; HEXURONIC ACID; MICROCAPSULE;

ARTICLE; BLOOD; BLOOD CULTURE; CELL ENCAPSULATION; CELL FUNCTION; CELL PROLIFERATION; CELL THERAPY; CELL VIABILITY; CONTROLLED STUDY; EX VIVO STUDY; GRANULOCYTE; HEK293 CELL LINE; HUMAN; HUMAN CELL; IMMUNOCOMPETENT CELL; LEUKOCYTE; LEUKOCYTE ACTIVATION; LYMPHOCYTE; NONHUMAN; PROTEIN EXPRESSION; WHOLE BLOOD CULTURE; BLOOD CELL; CELL CULTURE TECHNIQUE; CELL SURVIVAL; COCULTURE; CYTOLOGY; METABOLISM; MICROCAPSULE; PROCEDURES;

ALGINATES; BIOCOMPATIBLE MATERIALS; BLOOD CELLS; CAPSULES; CELL CULTURE TECHNIQUES; CELL PHYSIOLOGICAL PHENOMENA; CELL SURVIVAL; COCULTURE TECHNIQUES; CYTOLOGICAL TECHNIQUES; GLUCURONIC ACID; HEXURONIC ACIDS; HUMANS;

EID: 84955737203     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25828     Document Type: Article

References (38)

1. Auslander D, Auslander S, Charpin-El Hamri G, Sedlmayer F, Muller M, Frey O, Hierlemann A, Stelling J, Fussenegger M. 2014. A synthetic multifunctional mammalian pH sensor and CO2 transgene-control device. Mol Cell 55(3):397-408.
2. Auslander D, Eggerschwiler B, Kemmer C, Geering B, Auslander S, Fussenegger M. 2014. A designer cell-based histamine-specific human allergy profiler. Nat Commun 5:4408.
3. Boehler RM, Graham JG, Shea LD. 2011. Tissue engineering tools for modulation of the immune response. Biotechniques 51(4):239-240. 242, 244 passim.
4. Damsgaard CT, Lauritzen L, Calder PC, Kjaer TMR, Frokiaer H. 2009. Whole-blood culture is a valid low-cost method to measure monocytic cytokines-A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes. J Immunol Methods 340(2):95-101.
5. Geering B, Fussenegger M. 2015. Synthetic immunology: Modulating the human immune system. Trends Biotechnol 33(2):65-79.
6. Geering B, Gurzeler U, Federzoni E, Kaufmann T, Simon HU. 2011. A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils. Blood 117(22):5953-5962.
7. Godoy-Ramirez K, Franck K, Mahdavifar S, Andersson L, Gaines H. 2004. Optimum culture conditions for specific and nonspecific activation of whole blood and PBMC for intracellular cytokine assessment by flow cytometry. J Immunol Methods 292(1-2):1-15.
8. Hodge G, Hodge S, Han P. 2000. Increased levels of apoptosis of leukocyte subsets in cultured PBMCs compared to whole blood as shown by annexin V binding: Relevance to cytokine production. Cytokine 12(12):1763-1768.
9. Ingulli E. 2010. Mechanism of cellular rejection in transplantation. Pediatr Nephrol 25(1):61-74.
10. Jacobs-Tulleneers-Thevissen D, Chintinne M, Ling Z, Gillard P, Schoonjans L, Delvaux G, Strand BL, Gorus F, Keymeulen B, Pipeleers D. 2013. Sustained function of alginate-encapsulated human islet cell implants in the peritoneal cavity of mice leading to a pilot study in a type 1 diabetic patient. Diabetologia 56(7):1605-1614.
11. Kaminska T, Wysocka A, Marmurowska-Michalowska H, Dubas-Slemp H, Kandefer-Szerszen M. 2001. Investigation of serum cytokine levels and cytokine production in whole blood cultures of paranoid schizophrenic patients. Arch Immunol Ther Exp 49(6):439-445.
12. Louis E, Franchimont D, Piron A, Gevaert Y, Schaaf-Lafontaine N, Roland S, Mahieu P, Malaise M, De Groote D, Louis R, Belaiche J. 1998. Tumour necrosis factor (TNF) gene polymorphism influences TNF-alpha production in lipopolysaccharide (LPS)-stimulated whole blood cell culture in healthy humans. Clin Exp Immunol 113(3):401-406.
13. Lyons AB. 2000. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J Immunol Methods 243(1-2):147-154.
14. Mak IWY, Evaniew N, Ghert M. 2014. Lost in translation: Animal models and clinical trials in cancer treatment. Am J Transl Res 6(2):114-118.
15. Mallone R, Mannering SI, Brooks-Worrell BM, Durinovic-Bello I, Cilio CM, Wong FS, Schloot NC. 2011. Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: Position statement of the T-cell workshop committee of the immunology of diabetes society. Clin Exp Immunol 163(1):33-49.
16. McFarlane SM, Pashmi G, Connell MC, Littlejohn AF, Tucker SJ, Vandenabeele P, MacEwan DJ. 2002. Differential activation of nuclear factor-kappa B by tumour necrosis factor receptor subtypes. TNFR1 predominates whereas TNFR2 activates transcription poorly. Febs Lett 515(1-3):119-126.
17. McNamee JP, Bellier PV, Kutzner BC, Wilkins RC. 2005. Effect of pro-inflammatory cytokines on spontaneous apoptosis in leukocyte sub-sets within a whole blood culture. Cytokine 31(2):161-167.
18. Mikolajczyk TP, Skrzeczynska-Moncznik JE, Zarebski MA, Marewicz EA, Wisniewska AM, Dzieba M, Dobrucki JW, Pryjma JR. 2009. Interaction of human peripheral blood monocytes with apoptotic polymorphonuclear cells. Immunology 128(1):103-113.
19. Oliver JC, Bland LA, Oettinger CW, Arduino MJ, McAllister SK, Aguero SM, Favero MS. 1993. Cytokine kinetics in an in vitro whole blood model following an endotoxin challenge. Lymphokine Cytokine Res 12(2):115-120.
20. Orive G, Tam SK, Pedraz JL, Halle JP. 2006. Biocompatibility of alginate-poly-L-lysine microcapsules for cell therapy. Biomaterials 27(20):3691-3700.
21. Paredes Juarez GA, Spasojevic M, Faas MM, de Vos P. 2014. Immunological and technical considerations in application of alginate-based microencapsulation systems. Front Bioeng Biotechnol 2:26.
22. Pauly JL, Sokal JE, Han T. 1973. Whole-blood culture technique for functional studies of lymphocyte reactivity to mitogens, antigens, and homologous lymphocytes. J Lab Clin Med 82(3):500-512.
23. Rossger K, Charpin-El Hamri G, Fussenegger M. 2013. Reward-based hypertension control by a synthetic brain-dopamine interface. Proc Natl Acad Sci USA 110(45):18150-18155.
24. Rossger K, Charpin-El-Hamri G, Fussenegger M. 2013b. A closed-loop synthetic gene circuit for the treatment of diet-induced obesity in mice. Nat Commun 4:2825.
25. Schmidt JJ, Rowley J, Kong HJ. 2008. Hydrogels used for cell-based drug delivery. J Biomed Mater Res A 87A(4):1113-1122.
26. Seok J, Warren HS, Cuenca AG, Mindrinos MN, Baker HV, Xu WH, Richards DR, McDonald-Smith GP, Gao H, Hennessy L, Finnerty CC, Lopez CM, Honari S, Moore EE, Minei JP, Cuschieri J, Bankey PE, Johnson JL, Sperry J, Nathens AB, Billiar TR, West MA, Jeschke MG, Klein MB, Gamelli RL, Gibran NS, Brownstein BH, Miller-Graziano C, Calvano SE, Mason PH, Cobb JP, Rahme LG, Lowry SF, Maier RV, Moldawer LL, Herndon DN, Davis RW, Xiao WZ, Tompkins RG. 2013. Genomic responses in mouse models poorly mimic human inflammatory diseases. Proc Natl Acad Sci USA 110(9):3507-3512.
27. Sottong PR, Rosebrock JA, Britz JA, Kramer TR. 2000. Measurement of T-lymphocyte responses in whole-blood cultures using newly synthesized DNA and ATP. Clin Diagn Lab Immunol 7(2):307-311.
28. Swaak AJG, vandenBrink HG, Aarden LA. 1997. Cytokine production in whole blood cell cultures of patients with rheumatoid arthritis. Ann Rheum Dis 56(11):693-695.
29. Vignais PV. 2002. The superoxide-generating NADPH oxidase: Structural aspects and activation mechanism. Cell Mol Life Sci 59(9):1428-1459.
30. Wallis RS, Jakubiec W, Mitton-Fry M, Ladutko L, Campbell S, Paige D, Silvia A, Miller PF. 2012. Rapid evaluation in whole blood culture of regimens for XDR-TB containing PNU-100480 (sutezolid), TMC207, PA-824, SQ109, and pyrazinamide. PLoS ONE 7(1):e30479.
31. Weir RE, Brennan PJ, Butlin CR, Dockrell HM. 1999. Use of a whole blood assay to evaluate in vitro T cell responses to new leprosy skin test antigens in leprosy patients and healthy subjects. Clin Exp Immunol 116(2):263-269.
32. Wilson BMG, Severn A, Rapson NT, Chana J, Hopkins P. 1991. A convenient human whole-blood culture system for studying the regulation of tumor-necrosis-factor release by bacterial lipopolysaccharide. J Immunol Methods 139(2):233-240.
33. Yachie A, Takano N, Yokoi T, Kato K, Kasahara Y, Miyawaki T, Taniguchi N. 1990. The capability of neonatal leukocytes to produce il-6 on stimulation assessed by whole-blood culture. Pediatr Res 27(3):227-233.
34. Yaqoob P, Newsholme EA, Calder PC. 1999. Comparison of cytokine production in cultures of whole human blood and purified mononuclear cells. Cytokine 11(8):600-605.
35. Ye HF, Daoud-El Baba M, Peng RW, Fussenegger M. 2011. A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice. Science 332(6037):1565-1568.
36. Zakharyan R, Boyajyan A. 2014. Inflammatory cytokine network in schizophrenia. World J Biol Psychia 15(3):174-187.
37. Zangerle PF, De Groote D, Lopez M, Meuleman RJ, Vrindts Y, Fauchet F, Dehart I, Jadoul M, Radoux D, Franchimont P. 1992a. Direct stimulation of cytokines (IL-1 beta, TNF-alpha, IL-6, IL-2, IFN-gamma and GM-CSF) in whole blood: II. Application to rheumatoid arthritis and osteoarthritis. Cytokine 4(6):568-575.
38. Zangerle PF, Degroote D, Lopez M, Meuleman RJ, Vrindts Y, Fauchet F, Dehart I, Jadoul M, Radoux D, Franchimont P. 1992b. Direct stimulation of cytokines (Il-1-Beta, Tnf-Alpha, Il-6, Il-2, Ifn-gamma and Gm-Csf) in whole-blood. 2. Application to rheumatoid-arthritis and osteoarthritis. Cytokine 4(6):568-575.