Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution

Journal of Immunological Methods

Volumn 243, Issue 1-2, 2000, Pages 147-154

  Lyons, A Bruce ^a  

^a UNIVERSITY OF TASMANIA   (Australia)

Author keywords

CFDA SE; CFSE; Differentiation; Division; Immune response; Kinetics

Indexed keywords

ACETOACETIC ACID; CARBOXYFLUORESCEIN; ESTER; FLUORESCENT DYE; SUCCINIMIDE;

ANIMAL CELL; ARTICLE; B LYMPHOCYTE; CELL DIFFERENTIATION; CELL DIVISION; CELL PROLIFERATION; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; IMMUNE RESPONSE; KINETICS; LYMPHOCYTE PROLIFERATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPLEEN CELL; T LYMPHOCYTE;

ANIMAL; CELL DIFFERENTIATION; CELL DIVISION; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; HUMAN; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTES; MEMBRANE PROTEINS; MICE; SUCCINIMIDES; SUPPORT, NON-U.S. GOV'T;

EID: 0034699367     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00231-3     Document Type: Article

References (17)

1. Angulo R., Fulcher D.A. Measurement of Candida-specific blastogenesis: comparison of carboxyfluorescein succinimidyl ester labelling of T cells, thymidine incorporation, and CD69 expression. Cytometry. 34:1998;143.
2. Bird J.J., Brown D.R., Mullen A.C., Moskowitz N.H., Mahowald M.A., Sider J.R., Gajewski T.F., Wang C.-R., Reiner S.L. Helper T cell differentiation is controlled by the cell cycle. Immunity. 9:1998;229.
3. Fulcher D.A., Lyons A.B., Korn S., Cook M.C., Koleda C., Parish C., Fazekas de St. Groth B., Basten A. The fate of self-reactive B-cells depends primarily on the degree of antigen receptor engagement and availability of T-cell help. J. Exp. Med. 183:1996;2313.
4. Gett A.V., Hodgkin P.D. Cell division regulates the T cell cytokine repertoire, revealing a mechanism underlying immune class regulation. Proc. Natl. Acad. Sci. USA. 95:1998;9488.
5. Hasbold J., Lyons A.B., Kehry M.R., Hodgkin P.D. Cell division number regulates IgG1 and IgE switching of B cells following CD40L and IL-4 stimulation. Eur. J. Immunol. 28:1998;1040.
6. Hodgkin P.D., Lee J.H., Lyons A.B. B cell differentiation and isotype switching is related to division cycle number. J. Exp. Med. 184:1996;277.
7. Hodgkin P.D., Bartell G., Mamchak A., Doherty K.V., Lyons A.B., Hasbold J. The importance of efficacy and partial agonism in evaluating models of B lymphocyte activation. Int. Rev. Immunol. 15:1997;101.
8. Kindler V., Zubler R.H. Memory, but not naive, peripheral blood B lymphocytes differentiate into Ig-secreting cells after CD40 ligation and costimulation with IL-4 and the differentiation factors IL-2, IL-10, and IL-3. J. Immunol. 159:1997;2085.
9. Kurts C., Carbone F.R., Barnden M., Blanas E., Allison J., Heath W.R., Miller J.F. CD4+ T cell help impairs CD8+ T cell deletion induced by cross-presentation of self-antigens and favors autoimmunity. J. Exp. Med. 186:1997;2057.
10. Lee W.T., Pelletier W.J. Visualizing memory phenotype development after in vitro stimulation of CD4+ T cells. Cell. Immunol. 188:1998;1.
11. Lyons A.B. Pertussis toxin pretreatment alters the in vivo division behaviour and survival of B lymphocytes, after intravenous transfer. Immunol. Cell Biol. 75:1997;7.
12. Lyons A.B., Doherty K.V. Flow cytometric analysis of cell division by dye dilution. Robinson J.P., et al. Current Protocols in Cytometry. 1998;Wiley, New York. Unit 9.11.
13. Lyons A.B., Hasbold J., Hodgkin P.D. Flow cytometric analysis of cell division history using dilution of CFSE, a stably integrated fluorescent probe. Darzynkiewicz Z., et al. Methods in Cell Biology; Cytometry. 2000;Academic Press, San Diego. in press.
14. Lyons A.B., Parish C.R. Determination of lymphocyte division by flow cytometry. J. Immunol. Methods. 171:1994;131.
15. Nordon R.E., Ginsberg S.S., Eaves C.J. High resolution cell division tracking demonstrates the Flt3 ligand dependence of human marrow CD34+CD38- cell production in vitro. Br. J. Haematol. 98:1997;528.
16. Warren H.S., Kinnear B.F. Quantitative analysis of the effect of CD16 ligation on human NK cell proliferation. J. Immunol. 162:1999;735.
17. Wells A.D., Gudmundsdottir H., Turka L.A. Following the fate of individual T cells throughout activation and clonal expansion. Signals from T cell receptor and CD28 differentially regulate the induction and duration of a proliferative response. J. Clin. Invest. 100:1997;3173.