The production of multiprotein complexes in insect cells using the baculovirus expression system

Methods in Molecular Biology

Volumn 1261, Issue , 2015, Pages 91-114

  Abdulrahman, Wassim ^a   Radu, Laura ^a   Garzoni, Frederic ^b   Kolesnikova, Olga ^a   Gupta, Kapil ^b   Osz Papai, Judit ^a   Berger, Imre ^b   Poterszman, Arnaud ^a  

^a INSERM   (France)

^b UNIV GRENOBLE ALPES   (France)

Author keywords

Baculovirus; BEVS; Infection and coinfection methods; Insect cells; Multigene expression; Multiprotein complex; Recombinant protein complex production

Indexed keywords

DNA FRAGMENT; MULTIPROTEIN COMPLEX; RECOMBINANT PROTEIN;

ARTICLE; BACULOVIRUS; BACULOVIRUS EXPRESSION SYSTEM; CELL DENSITY; CELL VIABILITY; CONTROLLED STUDY; GENETIC TRANSFECTION; GROWTH; HOMOLOGOUS RECOMBINATION; INSECT CELL; LOXP SITE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; RESTRICTION SITE; SEQUENCE ALIGNMENT; VIRUS REPLICATION; ANIMAL; BACULOVIRIDAE; BIOSYNTHESIS; CELL LINE; CULTURE TECHNIQUE; CYTOLOGY; GENE VECTOR; GENETICS; INSECT; METABOLISM; MOLECULAR CLONING; PROCEDURES; VIROLOGY;

EUKARYOTA; HEXAPODA;

ANIMALS; BACULOVIRIDAE; CELL CULTURE TECHNIQUES; CELL LINE; CLONING, MOLECULAR; GENETIC VECTORS; INSECTS; MULTIPROTEIN COMPLEXES; RECOMBINANT PROTEINS;

EID: 84954601111     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2230-7_5     Document Type: Article

References (16)

1. Baneyx F (1999) Recombinant protein expression in Escherichia coli. Curr Opin Biotechnol 10:411–421.
2. Brondyk WH (2009) Selecting an appropriate method for expressing a recombinant protein. Methods Enzymol 463:131–147.
3. Assenberg R, Wan P, Geisse S, Mayr L (2013) Advances in recombinant protein expression for use in pharmaceutical research. Curr Opin Struct Biol 23:393–402.
4. Khan KH (2013) Gene expression in mammalian cells and its applications. Adv Pharm Bull 3:257–263.
5. Picanco-Castro V, Biaggio RT, Cova DT, Swiech K (2013) Production of recombinant therapeutic proteins in human cells: current achievements and future perspectives. Protein Pept Lett 20:1373–1381.
6. Fitzgerald DJ, Berger P, Schaffi tzel C et al (2006) Protein complex expression by using multigene baculoviral vectors. Nat Methods 3:1021–1032.
7. Berger I, Fitzgerald DJ, Richmond TJ (2004) Baculovirus expression system for heterologous multiprotein complexes. Nat Biotechnol 22:1583–1587.
8. Bieniossek C, Imasaki T, Takagi Y, Berger I (2012) MultiBac: expanding the research toolbox for multiprotein complexes. Trends Biochem Sci 37:49–57.
9. Sokolenko S, George S, Wagner A et al (2012) Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: benefi ts and drawbacks. Biotechnol Adv 30:766–781.
10. Belyaev AS, Roy P (1993) Development of baculovirus triple and quadruple expression vectors: co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells. Nucleic Acids Res 21:1219–1223.
11. Abdulrahman W, Uhring M, Kolb-Cheynelet I et al (2009) A set of baculovirus transfer vectors for screening of affi nity tags and parallel expression strategies. Anal Biochem 385:383–385.
12. Nie Y, Bellon-Echeverria I, Trowitzsch S et al (2014) Multiprotein complex production in insect cells by using polyproteins. Methods Mol Biol 1091:131–141.
13. Zhao Y, Chapman DA, Jones IM (2003) Improving baculovirus recombination. Nucleic Acids Res 31:E6–6.
14. Kool M, van Lier FLJ, Vlak JM, Tramper J (1991) Detection and analysis of Autographa californica nuclear polyhedrosis virus mutants with defective interfering properties. Virology 183:739–746.
15. Vijayachandran LS, Viola C, Garzoni F et al (2011) Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells. J Struct Biol 175:198–208.
16. Pijlman GP, van den Born E, Martens DE, Vlak JM (2001) Autographa californica baculoviruses with large genomic deletions are rapidly generated in infected insect cells. Virology 283:132–138