Constructing a synthetic constitutive metabolic pathway in Escherichia coli for (R, R)-2,3-butanediol production

Applied Microbiology and Biotechnology

Volumn 100, Issue 2, 2016, Pages 637-647

  Tong, Ying Jia ^a   Ji, Xiao Jun ^a   Shen, Meng Qiu ^a   Liu, Lu Gang ^a   Nie, Zhi Kui ^a   Huang, He ^a  

^a NANJING TECH UNIVERSITY   (China)

Author keywords

(R, R) 2,3 butanediol; Escherichia coli; Metabolic engineering; Promoter

Indexed keywords

ACETIC ACID; BIOCHEMISTRY; FERMENTATION; GENE EXPRESSION; GENES; GLUCOSE; METABOLIC ENGINEERING; METABOLISM; ORGANIC ACIDS; PH;

(R, R)-2,3-BUTANEDIOL; FERMENTATION CONDITIONS; GLUCOSE CONSUMPTION RATE; LARGE SCALE PRODUCTIONS; METABOLIC PATHWAYS; PROMOTER; RECOMBINANT STRAINS; SPATIAL CONFIGURATION;

ESCHERICHIA COLI;

2,3 BUTANEDIOL; ACETIC ACID; ACETOIN; LAC REPRESSOR; 2,3-BUTYLENE GLYCOL; BUTANEDIOL; GLUCOSE;

BIOTECHNOLOGY; COLIFORM BACTERIUM; FERMENTATION; GENE EXPRESSION; METABOLISM;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CELL DENSITY; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; FERMENTATION; FERMENTATION OPTIMIZATION; GENE CLUSTER; GENE OVEREXPRESSION; GLUCOSE INTAKE; METABOLIC ENGINEERING; NONHUMAN; OPERON; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; BATCH CELL CULTURE; GENETICS; LACTOSE OPERON; METABOLISM; MULTIGENE FAMILY; PROCEDURES; SYNTHETIC BIOLOGY;

ESCHERICHIA COLI;

ACETOIN; BATCH CELL CULTURE TECHNIQUES; BUTYLENE GLYCOLS; ESCHERICHIA COLI; FERMENTATION; GLUCOSE; LAC OPERON; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; MULTIGENE FAMILY; PROMOTER REGIONS, GENETIC; SYNTHETIC BIOLOGY;

EID: 84953639059     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-7013-3     Document Type: Article

References (23)

1. Alper H, Fischer C, Nevoigt E, Stephanopoulos G (2005) Tuning genetic control through promoter engineering. Proc Natl Acad Sci U S A 102:12678–12683
2. Celinska E, Grajek W (2009) Biotechnological production of 2,3-butanediol—current state and prospects. Biotechnol Adv 27:715–725
3. Chen C, Wei D, Shi JP, Wang M, Hao J (2014) Mechanism of 2,3-butanediol stereoisomer formation in Klebsiella pneumoniae. Appl Microbiol Biotechnol 98:4603–4613
4. Chong L (2001) Molecular cloning—a laboratory manual, 3rd edition. Science 292:446
5. Dai JJ, Cheng JS, Liang YQ, Jiang T, Yuan YJ (2014) Regulation of extracellular oxidoreduction potential enhanced (R, R)-2,3-butanediol production by Paenibacillus polymyxa CJX518. Bioresour Technol 167:433–440
6. Fu J, Wang Z, Chen T, Liu W, Shi T, Wang G, Y-j T, Zhao X (2014) NADH plays the vital role for chiral pure D-(−)-2,3-butanediol production in Bacillus subtilis under limited oxygen conditions. Biotechnol Bioeng 111:2126–2131
7. Ji X-J, Huang H, Ouyang P-K (2011) Microbial 2,3-butanediol production: a state-of-the-art review. Biotechnol Adv 29:351–364
8. Ji X-J, Liu L-G, Shen M-Q, Nie Z-K, Tong Y-J, Huang H (2015) Constructing a synthetic metabolic pathway in Escherichia coli to produce the enantiomerically pure (R, R)-2,3-butanediol. Biotechnol Bioeng 112:1056–1059
9. Kim KJ, Kim HE, Lee KH, Han W, Yi MJ, Jeong J, Oh BH (2004) Two-promoter vector is highly efficient for overproduction of protein complexes. Protein Sci 13:1698–1703
10. Kim D-K, Rathnasingh C, Song H, Lee HJ, Seung D, Chang YK (2013) Metabolic engineering of a novel Klebsiella oxytoca strain for enhanced 2,3-butanediol production. J Biosci Bioeng 116:186–192
11. Li Z-J, Jian J, Wei X-X, Shen X-W, Chen G-Q (2010) Microbial production of meso-2,3-butanediol by metabolically engineered Escherichia coli under low oxygen condition. Appl Microbiol Biotechnol 87:2001–2009
12. Li L, Li K, Wang Y, Chen C, Xu Y, Zhang L, Han B, Gao C, Tao F, Ma C, Xu P (2015) Metabolic engineering of Enterobacter cloacae for high-yield production of enantiopure (2R,3R)-2,3-butanediol from lignocellulose-derived sugars. Metab Eng 28:19–27
13. Lu C, Jeffries T (2007) Shuffling of promoters for multiple genes to optimize xylose fermentation in an engineered Saccharomyces cerevisiae strain. Appl Environ Microb 73:6072–6077
14. Sadhu KM, Matteson DS, Hurst GD, Kurosky JM (1984) (R,R)-2,3-Butanediol as chiral directing group in the synthesis of (Alpha-S)-Alpha-chloro boronic esters. Organometallics 3:804–806
15. Sandoval NR, Mills TY, Zhang M, Gill RT (2011) Elucidating acetate tolerance in E. coli using a genome-wide approach. Metab Eng 13:214–224
16. Snoep JL, Yomano LP, Westerhoff HV, Ingram LO (1995) Protein burden in Zymomonas mobilis: negative flux and growth control due to overproduction of glycolytic enzymes. Microbiology 141:2329–2337
17. Ui S, Masuda T, Masuda H, Muraki H (1986) Mechanism for the formation of 2,3-butanediol stereoisomers in Bacillus polymyxa. J Ferment Technol 64:481–486
18. Ui S, Okajima Y, Mimura A, Kanai H, Kudo T (1997) Molecular generation of an Escherichia coli strain producing only the meso-isomer of 2,3-butanediol. J Ferment Bioeng 84:185–189
19. Ui S, Takusagawa Y, Sato T, Ohtsuki T, Mimura A, Ohkuma M, Kudo T (2004) Production of L-2,3-butanediol by a new pathway constructed in Escherichia coli. Lett Appl Microbiol 39:533–537
20. Vieira J, Messing J (1991) New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100:189–194
21. Xu Y, Chu H, Gao C, Tao F, Zhou Z, Li K, Li L, Ma C, Xu P (2014) Systematic metabolic engineering of Escherichia coli for high-yield production of fuel bio-chemical 2,3-butanediol. Metab Eng 23:22–33
22. Yan Y, Lee C-C, Liao JC (2009) Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases. Org Biomol Chem 7:3914–3917
23. Zeng A-P, Sabra W (2011) Microbial production of diols as platform chemicals: recent progresses. Curr Opin Biotechnol 22:749–757