Redirection of Metabolic Flux into Novel Gamma-Aminobutyric Acid Production Pathway by Introduction of Synthetic Scaffolds Strategy in Escherichia Coli

Applied Biochemistry and Biotechnology

Volumn 178, Issue 7, 2016, Pages 1315-1324

  Pham, Van Dung ^a   Somasundaram, Sivachandiran ^a   Lee, Seung Hwan ^b   Park, Si Jae ^c   Hong, Soon Ho ^a  

^a University of Ulsan   (South Korea)

^b Chonnam National University   (South Korea)

^c Myong Ji University   (South Korea)

Author keywords

Gamma aminobutyric acid; Glucose; Metabolic pathway; Recombinant DNA; Synthetic scaffold

Indexed keywords

AMINO ACIDS; CARBOXYLATION; ESCHERICHIA COLI; GLUCOSE; MOLECULAR WEIGHT; SCAFFOLDS;

CORYNEBACTERIUM; GABA AMINOTRANSFERASE; GAMMA-AMINOBUTYRIC ACIDS; METABOLIC PATHWAYS; RECOMBINANT DNA; SEMIALDEHYDE DEHYDROGENASE; SUCCINATE DEHYDROGENASE; SYNTHETIC SCAFFOLDS;

METABOLISM;

4 AMINOBUTYRATE AMINOTRANSFERASE; 4 AMINOBUTYRIC ACID; ACETATE KINASE; FORMIC ACID; FUMARATE REDUCTASE; GBD PROTEIN; GLUCOSE; LACTATE DEHYDROGENASE; PDZ PROTEIN; PROTEIN; PROTEIN SH3; PYRUVATE OXIDASE; PYRUVIC ACID; SUCCINATE DEHYDROGENASE; SUCCINATE SEMIALDEHYDE DEHYDROGENASE; SUCCINIC ACID; SUCCINIC SEMIALDEHYDE; UNCLASSIFIED DRUG; GLUTAMIC ACID;

ARTICLE; CITRIC ACID CYCLE; ESCHERICHIA COLI; METABOLISM; NONHUMAN; PDZ DOMAIN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; TEMPERATURE SENSITIVITY; BIOSYNTHESIS; FERMENTATION; GENETICS; METABOLIC ENGINEERING;

CITRIC ACID CYCLE; ESCHERICHIA COLI; FERMENTATION; GAMMA-AMINOBUTYRIC ACID; GLUCOSE; GLUTAMIC ACID; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS;

EID: 84949788135     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-015-1948-9     Document Type: Article

References (20)

1. Datsenko, K. A., & Wanner, B. L. (2000). One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proceedings of the National Academy of Science of the United States of America, 97, 6640–6645.
2. Delebecque, C. J., Lindner, A. B., Silver, P. A., & Aldaye, F. A. (2011). Organization of intracellular reactions with rationally designed RNA assemblies. Science, 333, 470–474.
3. Dueber, J. E., Wu, G. C., Malmirchegini, G. R., Moon, T. S., Petzold, C. J., Ullal, A. V., Prather, K. L. J., & Keasling, J. D. (2009). Synthetic protein scaffolds provide modular control over metabolic flux. Nature Biotechnology, 27, 753–759.
4. Good, M. C., Zalatan, J. G. and Lim, W. A. (2011). Scaffold proteins: hubs for controlling the flow of cellular information. Science (New York, N.Y.) 332, 680–686.
5. Guzman, L. M., Belin, D., Carson, M. J., & Beckwith, J. (1995). Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. Journal of Bacteriology, 177, 4121–4130.
6. Hirst, J., Sucheta, A., Ackrell, B. A. C., & Armstrong, F. A. (1996). Electrocatalytic voltammetry of succinate dehydrogenase: direct quantification of the catalytic properties of a complex electron-transport enzyme. Journal of the American Chemical Society, 118, 5031–5038.
7. Ignacio Tinoco Jr. KS, J. C. W., Joseph D. Puglisi, Gerard Harbison, David Rovnyak. (2002). Physical chemistry: principle and applications in biological sciences, 4th. Prentice Hall, Upper Saddle River, NJ.
8. Kook, M.-C., Seo, M.-J., Cheigh, C.-I., Lee, S.-J., Pyun, Y.-R., & Park, H. (2010). Enhancement of γ-amminobutyric acid production by Lactobacillus sakei B2–16 expressing glutamate decarboxylase from Lactobacillus plantarum ATCC 14917. Journal of the Korean Society Applied Biological Chemistry, 53, 816–820.
9. Le Vo, T., Ko, J.-s., Lee, S., Park, S. and Hong, S. (2013). Overexpression of Neurospora crassa OR74A glutamate decarboxylase in Escherichia coli for efficient GABA production. Biotechnology and Bioprocess Engineering, 18, 1062–1066.
10. Le Vo, T., Ko, J.-s., Park, S., Lee, S. and Hong, S. (2013). Efficient gamma-aminobutyric acid bioconversion by employing synthetic complex between glutamate decarboxylase and glutamate/GABA antiporter in engineered Escherichia coli. Journal of Industrial Microbiology and Biotechnology, 40, 927–933.
11. Lederer, T., Kintrup, M., Takahashi, M., Sum, P.-E., Ellestad, G. A., & Hillen, W. (1996). Tetracycline analogs affecting binding to Tn10-encoded Tet repressor trigger the same mechanism of induction. Biochemistry, 35, 7439–7446.
12. Maklashina, E., Berthold, D. A., & Cecchini, G. (1998). Anaerobic expression of Escherichia coli succinate dehydrogenase: functional replacement of fumarate reductase in the respiratory chain during anaerobic growth. Journal of Bacteriology, 180, 5989–5996.
13. Moon, T. S., Dueber, J. E., Shiue, E., & Prather, K. L. J. (2010). Use of modular, synthetic scaffolds for improved production of glucaric acid in engineered E. coli. Metabolic Engineering, 12, 298–305.
14. Oh CH, O. S. (2004). Effect of germinated brown rice extracts with enhanced levels of GABA on cancer cell proliferation and apoptosis. Journal of Medicinal Food, 7, 19.
15. Park, K.-B., & Oh, S.-H. (2006). Enhancement of γ-aminobutyric acid production in Chungkukjang by applying a Bacillus subtilis strain expressing glutamate decarboxylase from Lactobacillus brevis. Biotechnology Letters, 28, 1459–1463.
16. Park, S., Kim, E., Noh, W., Oh, Y., Kim, H., Song, B., Cho, K., Hong, S., Lee, S., & Jegal, J. (2013). Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli. Bioprocess and Biosystems Engineering, 36, 885–892.
17. Pham, V. D., Lee, S. H., Park, S. J., & Hong, S. H. (2015). Production of gamma-aminobutyric acid from glucose by introduction of synthetic scaffolds between isocitrate dehydrogenase, glutamate synthase and glutamate decarboxylase in recombinant Escherichia coli. Journal of Biotechnology, 207, 52–57.
18. Sambrook J, R. D. (2001) Molecular cloning: a laboratory manual.
19. Vo, T., Pham, V., Ko, J.-s., Lee, S., Park, S. and Hong, S. (2014). Improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli. Biotechnology and Bioprocess Engineering, 19, 327–331.
20. Vo, T. D., & Hong, S. H. (2012). Optimization of gamma-Aminobutyric acid (GABA) bioconversion by recombinant Escherichia coli. Korean Society for Biotechnology and Bioengineering Journal, 27, 127–130.