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Volumn 16, Issue 12, 2015, Pages 28765-28782

Next-generation sequencing workflow for NSCLC critical samples using a targeted sequencing approach by ion torrent PGM™ platform

Author keywords

CfDNA; FFPE; Ion Torrent PGM; Next generation sequencing; NGS workflow; NSCLC

Indexed keywords

GENOMIC DNA;

EID: 84949435541     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms161226129     Document Type: Article
Times cited : (35)

References (63)
  • 1
    • 84892805731 scopus 로고    scopus 로고
    • Cancer statistics, 2014
    • CrossRefPubMed
    • Siegel, R.; Ma, J.; Zou, Z.; Jemal, A. Cancer statistics, 2014. CA Cancer J. Clin. 2014, 64, 9–29. [CrossRef] [PubMed]
    • (2014) CA Cancer J. Clin , vol.64 , pp. 9-29
    • Siegel, R.1    Ma, J.2    Zou, Z.3    Jemal, A.4
  • 2
    • 4444344330 scopus 로고    scopus 로고
    • EGF receptor gene mutations are common in lung cancers from “never smokers” and are associated with sensitivity of tumors to gefitinib and erlotinib
    • CrossRefPubMed
    • Pao, W.; Miller, V.; Zakowski, M.; Doherty, J.; Politi, K.; Sarkaria, I.; Singh, B.; Heelan, R.; Rusch, V.; Fulton, L.; et al. EGF receptor gene mutations are common in lung cancers from “never smokers” and are associated with sensitivity of tumors to gefitinib and erlotinib. Proc. Natl. Acad. Sci. USA 2004, 101, 13306–13311. [CrossRef] [PubMed]
    • (2004) Proc. Natl. Acad. Sci. USA , vol.101 , pp. 13306-13311
    • Pao, W.1    Miller, V.2    Zakowski, M.3    Doherty, J.4    Politi, K.5    Sarkaria, I.6    Singh, B.7    Heelan, R.8    Rusch, V.9    Fulton, L.10
  • 4
    • 0017681196 scopus 로고
    • DNA sequencing with chain terminating inhibitors
    • CrossRefPubMed
    • Sanger, F.; Nicklen, S.; Coulson, A.R. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 1977, 74, 5463–5467. [CrossRef] [PubMed]
    • (1977) Proc. Natl. Acad. Sci. USA , vol.74 , pp. 5463-5467
    • Sanger, F.1    Nicklen, S.2    Coulson, A.R.3
  • 5
    • 15744381713 scopus 로고
    • Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions
    • CrossRefPubMed
    • Higuchi, R.; Fockler, C.; Dollinger, G.; Watson, R. Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions. Biotechnology 1993, 11, 1026–1030. [CrossRef] [PubMed]
    • (1993) Biotechnology , vol.11 , pp. 1026-1030
    • Higuchi, R.1    Fockler, C.2    Dollinger, G.3    Watson, R.4
  • 6
    • 0032540905 scopus 로고    scopus 로고
    • A sequencing method based on real-time pyrophosphate
    • CrossRefPubMed
    • Ronaghi, M.; Uhlén, M.; Nyrén, P. A sequencing method based on real-time pyrophosphate. Science 1998, 281, 363–365. [CrossRef] [PubMed]
    • (1998) Science , vol.281 , pp. 363-365
    • Ronaghi, M.1    Uhlén, M.2    Nyrén, P.3
  • 7
    • 84926653463 scopus 로고    scopus 로고
    • Performance of standard procedures in detection of EGFR mutations in daily practice in advanced NSCLC patients selected according to the ESMO guideline: A large Caucasian cohort study
    • CrossRefPubMed
    • Hantson, I.; Dooms, C.; Verbeken, E.; Vandenberghe, P.; Vliegen, L.; Roskams, T.; Borght, S.V.; Nackaerts, K.; Wauters, I.; Vansteenkiste, J. Performance of standard procedures in detection of EGFR mutations in daily practice in advanced NSCLC patients selected according to the ESMO guideline: A large Caucasian cohort study. Transl. Respir. Med. 2014, 2. [CrossRef] [PubMed]
    • (2014) Transl. Respir. Med , pp. 2
    • Hantson, I.1    Dooms, C.2    Verbeken, E.3    Vandenberghe, P.4    Vliegen, L.5    Roskams, T.6    Borght, S.V.7    Nackaerts, K.8    Wauters, I.9    Vansteenkiste, J.10
  • 8
    • 79958835059 scopus 로고    scopus 로고
    • Comprehensive next-generation cancer genome sequencing in the era of targeted therapy and personalized oncology
    • CrossRefPubMed
    • Cronin, M.; Ross, J.S. Comprehensive next-generation cancer genome sequencing in the era of targeted therapy and personalized oncology. Biomark. Med. 2011, 5, 293–305. [CrossRef] [PubMed]
    • (2011) Biomark. Med , vol.5 , pp. 293-305
    • Cronin, M.1    Ross, J.S.2
  • 9
    • 84936132730 scopus 로고    scopus 로고
    • BioCAST/IFCT-1002 investigators: Noninvasive diagnosis of actionable mutations by deep sequencing of circulating free DNA in lung cancer from never-smokers: A proof-of-concept study from BioCAST/IFCT-1002
    • CrossRefPubMed
    • Couraud, S.; Vaca-Paniagua, F.; Villar, S.; Oliver, J.; Schuster, T.; Blanché, H.; Girard, N.; Trédaniel, J.; Guilleminault, L.; Gervais, R.; et al. BioCAST/IFCT-1002 investigators: Noninvasive diagnosis of actionable mutations by deep sequencing of circulating free DNA in lung cancer from never-smokers: A proof-of-concept study from BioCAST/IFCT-1002. Clin. Cancer Res. 2014, 20, 4613–4624. [CrossRef] [PubMed]
    • (2014) Clin. Cancer Res , vol.20 , pp. 4613-4624
    • Couraud, S.1    Vaca-Paniagua, F.2    Villar, S.3    Oliver, J.4    Schuster, T.5    Blanché, H.6    Girard, N.7    Trédaniel, J.8    Guilleminault, L.9    Gervais, R.10
  • 10
    • 84949430259 scopus 로고    scopus 로고
    • Available online, accessed on 10 April 2015
    • Nomenclature for the description of sequence variants. Available online: http://www.hgvs.org/mutnomen/ (accessed on 10 April 2015).
  • 11
    • 0033987736 scopus 로고    scopus 로고
    • Mutation nomenclature extensions and suggestions to describe complex mutations: A discussion
    • CrossRef
    • Den Dunnen, J.T.; Antonarakis, S.E. Mutation nomenclature extensions and suggestions to describe complex mutations: A discussion. Hum. Mutat. 2000, 15, 7–12. [CrossRef]
    • (2000) Hum. Mutat , vol.15 , pp. 7-12
    • Den Dunnen, J.T.1    Antonarakis, S.E.2
  • 12
    • 84928209346 scopus 로고    scopus 로고
    • Standards and guidelines for the interpretation of sequence variants: A joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology
    • CrossRefPubMed
    • Richards, S.; Aziz, N.; Bale, S.; Bick, D.; Das, S.; Gastier-Foster, J.; Grody, W.W.; Hegde, M.; Lyon, E.; Spector, E.; et al. Standards and guidelines for the interpretation of sequence variants: A joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet. Med. 2015, 17, 405–424. [CrossRef] [PubMed]
    • (2015) Genet. Med , vol.17 , pp. 405-424
    • Richards, S.1    Aziz, N.2    Bale, S.3    Bick, D.4    Das, S.5    Gastier-Foster, J.6    Grody, W.W.7    Hegde, M.8    Lyon, E.9    Spector, E.10
  • 13
    • 84949450284 scopus 로고    scopus 로고
    • Available online, accessed on 28 February 2015
    • COSMIC (Catalogue of Somatic Mutations in Cancer). Available online: http://cancer.sanger.ac.uk/cosmic (accessed on 28 February 2015).
  • 14
    • 84964315804 scopus 로고    scopus 로고
    • Evaluation of an integrated clinical workflow for targeted next-generation sequencing of low-quality tumor DNA using a 51-gene enrichment panel
    • CrossRefPubMed
    • Choudhary, A.; Mambo, E.; Sanford, T.; Boedigheimer, M.; Twomey, B.; Califano, J.; Hadd, A.; Oliner, K.S.; Beaudenon, S.; Latham, G.J.; et al. Evaluation of an integrated clinical workflow for targeted next-generation sequencing of low-quality tumor DNA using a 51-gene enrichment panel. BMC Med. Genom. 2014, 7. [CrossRef] [PubMed]
    • (2014) BMC Med. Genom , pp. 7
    • Choudhary, A.1    Mambo, E.2    Sanford, T.3    Boedigheimer, M.4    Twomey, B.5    Califano, J.6    Hadd, A.7    Oliner, K.S.8    Beaudenon, S.9    Latham, G.J.10
  • 15
    • 84898464134 scopus 로고    scopus 로고
    • Profiling cancer gene mutations in clinical formalin-fixed, paraffin-embedded colorectal tumor specimens using targeted next-generation sequencing
    • CrossRefPubMed
    • Zhang, L.; Chen, L.; Sah, S.; Latham, G.J.; Patel, R.; Song, Q.; Koeppen, H.; Tam, R.; Schleifman, E.; Mashhedi, H.; et al. Profiling cancer gene mutations in clinical formalin-fixed, paraffin-embedded colorectal tumor specimens using targeted next-generation sequencing. Oncologist 2014, 19, 336–343. [CrossRef] [PubMed]
    • (2014) Oncologist , vol.19 , pp. 336-343
    • Zhang, L.1    Chen, L.2    Sah, S.3    Latham, G.J.4    Patel, R.5    Song, Q.6    Koeppen, H.7    Tam, R.8    Schleifman, E.9    Mashhedi, H.10
  • 16
    • 84874519595 scopus 로고    scopus 로고
    • Targeted, high-depth, next-generation sequencing of cancer genes in formalin-fixed, paraffin-embedded and fine-needle aspiration tumor specimens
    • CrossRefPubMed
    • Hadd, A.G.; Houghton, J.; Choudhary, A.; Sah, S.; Chen, L.; Marko, A.C.; Sanford, T.; Buddavarapu, K.; Krosting, J.; Garmirek, L.; et al. Targeted, high-depth, next-generation sequencing of cancer genes in formalin-fixed, paraffin-embedded and fine-needle aspiration tumor specimens. J. Mol. Diagn. 2013, 15, 234–247. [CrossRef] [PubMed]
    • (2013) J. Mol. Diagn. , vol.15 , pp. 234-247
    • Hadd, A.G.1    Houghton, J.2    Choudhary, A.3    Sah, S.4    Chen, L.5    Marko, A.C.6    Sanford, T.7    Buddavarapu, K.8    Krosting, J.9    Garmirek, L.10
  • 19
    • 84883178389 scopus 로고    scopus 로고
    • Quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies
    • CrossRefPubMed
    • Sah, S.; Chen, L.; Houghton, J.; Kemppainen, J.; Marko, A.C.; Zeigler, R.; Latham, G.J. Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies. Genome Med. 2013, 5, 77. [CrossRef] [PubMed]
    • (2013) Genome Med , vol.5 , pp. 77
    • Sah, S.1    Chen, L.2    Houghton, J.3    Kemppainen, J.4    Marko, A.C.5    Zeigler, R.6    Latham, G.J.7    Functional, D.8
  • 22
    • 84871227763 scopus 로고    scopus 로고
    • LoFreq: A sequence quality aware, ultra-sensitive variant caller for uncovering cell population heterogeneity from high-throughput sequencing datasets
    • CrossRefPubMed
    • Wilm, A.; Aw, P.P.; Bertrand, D.; Yeo, G.H.; Ong, S.H.; Wong, C.H.; Khor, C.C.; Petric, R.; Hibberd, M.L.; Nagarajan, N. LoFreq: A sequence quality aware, ultra-sensitive variant caller for uncovering cell population heterogeneity from high-throughput sequencing datasets. Nucleic Acids Res. 2012, 40, 11189–11201. [CrossRef] [PubMed]
    • (2012) Nucleic Acids Res , vol.40 , pp. 11189-11201
    • Wilm, A.1    Aw, P.P.2    Bertrand, D.3    Yeo, G.H.4    Ong, S.H.5    Wong, C.H.6    Khor, C.C.7    Petric, R.8    Hibberd, M.L.9    Nagarajan, N.10
  • 23
    • 84949470671 scopus 로고    scopus 로고
    • Available online, accessed on 3 February 2015
    • Genome Analysis Toolkit (GATK). Available online: https://www.broadinstitute.org/gatk/index.php (accessed on 3 February 2015).
  • 24
    • 84884900317 scopus 로고    scopus 로고
    • The new sequencer on the block: Comparison of Life Technology’s Proton sequencer to an Illumina HiSeq for whole-exome sequencing
    • CrossRefPubMed
    • Boland, J.F.; Chung, C.C.; Roberson, D.; Mitchell, J.; Zhang, X.; Im, K.M.; He, J.; Chanock, S.J.; Yeager, M.; Dean, M. The new sequencer on the block: Comparison of Life Technology’s Proton sequencer to an Illumina HiSeq for whole-exome sequencing. Hum. Genet. 2013, 132, 1153–1163. [CrossRef] [PubMed]
    • (2013) Hum. Genet , vol.132 , pp. 1153-1163
    • Boland, J.F.1    Chung, C.C.2    Roberson, D.3    Mitchell, J.4    Zhang, X.5    Im, K.M.6    He, J.7    Chanock, S.J.8    Yeager, M.9    Dean, M.10
  • 27
    • 43049132516 scopus 로고    scopus 로고
    • Tissue heterogeneity of EGFR mutation in lung adenocarcinoma
    • CrossRefPubMed
    • Sakurada, A.; Lara-Guerra, H.; Liu, N.; Shepherd, F.A.; Tsao, M.S. Tissue heterogeneity of EGFR mutation in lung adenocarcinoma. J. Thorac. Oncol. 2008, 3, 527–529. [CrossRef] [PubMed]
    • (2008) J. Thorac. Oncol , vol.3 , pp. 527-529
    • Sakurada, A.1    Lara-Guerra, H.2    Liu, N.3    Shepherd, F.A.4    Tsao, M.S.5
  • 28
    • 84907808265 scopus 로고    scopus 로고
    • Intratumor heterogeneity in localized lung adenocarcinomas delineated by multiregion sequencing
    • CrossRefPubMed
    • Zhang, J.; Fujimoto, J.; Zhang, J.; Wedge, D.C.; Song, X.; Zhang, J.; Seth, S.; Chow, C.W.; Cao, Y.; Gumbs, C.; et al. Intratumor heterogeneity in localized lung adenocarcinomas delineated by multiregion sequencing. Science 2014, 346, 256–259. [CrossRef] [PubMed]
    • (2014) Science , vol.346 , pp. 256-259
    • Zhang, J.1    Fujimoto, J.2    Zhang, J.3    Wedge, D.C.4    Song, X.5    Zhang, J.6    Seth, S.7    Chow, C.W.8    Cao, Y.9    Gumbs, C.10
  • 31
    • 84866894408 scopus 로고    scopus 로고
    • Comprehensive genomic characterization of squamous cell lung cancers
    • Cancer Genome Atlas Research Network. Comprehensive genomic characterization of squamous cell lung cancers. Nature 2012, 489, 519–525.
    • (2012) Nature , vol.489 , pp. 519-525
  • 32
    • 29944441900 scopus 로고    scopus 로고
    • Genetic polymorphisms in cell cycle regulatory genes MDM2 and TP53 are associated with susceptibility to lung cancer
    • CrossRefPubMed
    • Zhang, X.; Miao, X.; Guo, Y.; Tan, W.; Zhou, Y.; Sun, T.; Wang, Y.; Lin, D. Genetic polymorphisms in cell cycle regulatory genes MDM2 and TP53 are associated with susceptibility to lung cancer. Hum. Mutat. 2006, 27, 110–117. [CrossRef] [PubMed]
    • (2006) Hum. Mutat , vol.27 , pp. 110-117
    • Zhang, X.1    Miao, X.2    Guo, Y.3    Tan, W.4    Zhou, Y.5    Sun, T.6    Wang, Y.7    Lin, D.8
  • 33
    • 84918550287 scopus 로고    scopus 로고
    • Polymorphisms of p53 and MDM2 genes are associated with severe toxicities in patients with non-small cell lung cancer
    • CrossRefPubMed
    • Zheng, D.; Chen, Y.; Gao, C.; Wei, Y.; Cao, G.; Lu, N.; Hou, Y.; Jiang, X.; Wang, J. Polymorphisms of p53 and MDM2 genes are associated with severe toxicities in patients with non-small cell lung cancer. Cancer Biol. Ther. 2014, 15, 1542–1551. [CrossRef] [PubMed]
    • (2014) Cancer Biol. Ther , vol.15 , pp. 1542-1551
    • Zheng, D.1    Chen, Y.2    Gao, C.3    Wei, Y.4    Cao, G.5    Lu, N.6    Hou, Y.7    Jiang, X.8    Wang, J.9
  • 35
    • 84898542288 scopus 로고    scopus 로고
    • Liquid biopsies: Genotyping circulating tumor DNA
    • CrossRefPubMed
    • Diaz, L.A., Jr.; Bardelli, A. Liquid biopsies: Genotyping circulating tumor DNA. J. Clin. Oncol. 2014, 32, 579–586. [CrossRef] [PubMed]
    • (2014) J. Clin. Oncol , vol.32 , pp. 579-586
    • Diaz, L.A.1    Bardelli, A.2
  • 36
    • 0026755807 scopus 로고
    • Whole genome amplification from a single cell: Implications for genetic analysis
    • CrossRefPubMed
    • Zhang, L.; Cui, X.; Schmitt, K.; Hubert, R.; Navidi, W.; Arnheim, N. Whole genome amplification from a single cell: Implications for genetic analysis. Proc. Natl. Acad. Sci. USA 1992, 89, 5847–5851. [CrossRef] [PubMed]
    • (1992) Proc. Natl. Acad. Sci. USA , vol.89 , pp. 5847-5851
    • Zhang, L.1    Cui, X.2    Schmitt, K.3    Hubert, R.4    Navidi, W.5    Arnheim, N.6
  • 39
    • 0036020666 scopus 로고    scopus 로고
    • Rubicon Genomics
    • CrossRefPubMed
    • Langmore, J.P. Rubicon Genomics, Inc. Pharmacogenomics 2002, 3, 557–560. [CrossRef] [PubMed]
    • (2002) Inc. Pharmacogenomics , vol.3 , pp. 557-560
    • Langmore, J.P.1
  • 40
    • 0031831323 scopus 로고    scopus 로고
    • Mutation detection and single-molecule counting using isothermal rolling-circle amplification
    • CrossRefPubMed
    • Lizardi, P.M.; Huang, X.; Zhu, Z.; Bray-Ward, P.; Thomas, D.C.; Ward, D.C. Mutation detection and single-molecule counting using isothermal rolling-circle amplification. Nat. Genet. 1998, 19, 225–232. [CrossRef] [PubMed]
    • (1998) Nat. Genet , vol.19 , pp. 225-232
    • Lizardi, P.M.1    Huang, X.2    Zhu, Z.3    Bray-Ward, P.4    Thomas, D.C.5    Ward, D.C.6
  • 43
    • 24344436666 scopus 로고    scopus 로고
    • Comparison of yield and genotyping performance of multiple displacement amplification and OmniPlex whole genome amplified DNA generated from multiple DNA sources
    • CrossRefPubMed
    • Bergen, A.W.; Haque, K.A.; Qi, Y.; Beerman, M.B.; Garcia-Closas, M.; Rothman, N.; Chanock, S.J. Comparison of yield and genotyping performance of multiple displacement amplification and OmniPlex whole genome amplified DNA generated from multiple DNA sources. Hum. Mutat. 2005, 26, 262–270. [CrossRef] [PubMed]
    • (2005) Hum. Mutat , vol.26 , pp. 262-270
    • Bergen, A.W.1    Haque, K.A.2    Qi, Y.3    Beerman, M.B.4    Garcia-Closas, M.5    Rothman, N.6    Chanock, S.J.7
  • 44
    • 23044443627 scopus 로고    scopus 로고
    • Comparing whole-genome amplification methods and sources of biological samples for single-nucleotide polymorphism genotyping
    • CrossRefPubMed
    • Park, J.W.; Beaty, T.H.; Boyce, P.; Scott, A.F.; McIntosh, I. Comparing whole-genome amplification methods and sources of biological samples for single-nucleotide polymorphism genotyping. Clin. Chem. 2005, 51, 1520–1523. [CrossRef] [PubMed]
    • (2005) Clin. Chem , vol.51 , pp. 1520-1523
    • Park, J.W.1    Beaty, T.H.2    Boyce, P.3    Scott, A.F.4    McIntosh, I.5
  • 45
    • 19944432329 scopus 로고    scopus 로고
    • Investigating the utility of combining Phi29 whole genome amplification and highly multiplexed single nucleotide polymorphism BeadArray genotyping
    • CrossRefPubMed
    • Pask, R.; Rance, H.E.; Barratt, B.J.; Nutland, S.; Smyth, D.J.; Sebastian, M.; Twells, R.C.; Smith, A.; Lam, A.C.; Smink, L.J.; et al. Investigating the utility of combining Phi29 whole genome amplification and highly multiplexed single nucleotide polymorphism BeadArray genotyping. BMC Biotechnol. 2004, 4. [CrossRef] [PubMed]
    • (2004) BMC Biotechnol , pp. 4
    • Pask, R.1    Rance, H.E.2    Barratt, B.J.3    Nutland, S.4    Smyth, D.J.5    Sebastian, M.6    Twells, R.C.7    Smith, A.8    Lam, A.C.9    Smink, L.J.10
  • 47
    • 84861962107 scopus 로고    scopus 로고
    • Single cell genome sequencing
    • CrossRefPubMed
    • Yilmaz, S.; Singh, A.K. Single cell genome sequencing. Curr. Opin. Biotechnol. 2011, 23, 1–7. [CrossRef] [PubMed]
    • (2011) Curr. Opin. Biotechnol , vol.23 , pp. 1-7
    • Yilmaz, S.1    Singh, A.K.2
  • 48
    • 26844442640 scopus 로고    scopus 로고
    • Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance
    • CrossRefPubMed
    • Bergen, A.W.; Qi, Y.; Haque, K.A.; Welch, R.A.; Chanock, S.J. Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance. BMC Biotechnol. 2005, 5. [CrossRef] [PubMed]
    • (2005) BMC Biotechnol , pp. 5
    • Bergen, A.W.1    Qi, Y.2    Haque, K.A.3    Welch, R.A.4    Chanock, S.J.5
  • 49
    • 34548066536 scopus 로고    scopus 로고
    • DNA quantification of whole genome amplified samples for genotyping on a multiplexed bead array platform
    • CrossRefPubMed
    • Hansen, H.M.; Wiemels, J.L.; Wrensch, M.; Wiencke, J.K. DNA quantification of whole genome amplified samples for genotyping on a multiplexed bead array platform. Cancer Epidemiol. Biomark. Prev. 2007, 16, 1686–1690. [CrossRef] [PubMed]
    • (2007) Cancer Epidemiol. Biomark. Prev , vol.16 , pp. 1686-1690
    • Hansen, H.M.1    Wiemels, J.L.2    Wrensch, M.3    Wiencke, J.K.4
  • 52
    • 61549108028 scopus 로고    scopus 로고
    • Using whole genome amplification (WGA) of low-volume biopsies to assess the prognostic role of EGFR, KRAS, p53, and CMET mutations in advanced-stage non-small cell lung cancer (NSCLC)
    • CrossRefPubMed
    • Lim, E.H.; Zhang, S.L.; Li, J.L.; Yap, W.S.; Howe, T.C.; Tan, B.P.; Lee, Y.S.; Wong, D.; Khoo, K.L.; Seto, K.Y.; et al. Using whole genome amplification (WGA) of low-volume biopsies to assess the prognostic role of EGFR, KRAS, p53, and CMET mutations in advanced-stage non-small cell lung cancer (NSCLC). J. Thorac. Oncol. 2009, 4, 12–21. [CrossRef] [PubMed]
    • (2009) J. Thorac. Oncol , vol.4 , pp. 12-21
    • Lim, E.H.1    Zhang, S.L.2    Li, J.L.3    Yap, W.S.4    Howe, T.C.5    Tan, B.P.6    Lee, Y.S.7    Wong, D.8    Khoo, K.L.9    Seto, K.Y.10
  • 56
    • 84875634162 scopus 로고    scopus 로고
    • Integrative Genomics Viewer (IGV): High-performance genomics data visualization and exploration
    • CrossRefPubMed
    • Thorvaldsdóttir, H.; Robinson, J.T.; Mesirov, J.P. Integrative Genomics Viewer (IGV): High-performance genomics data visualization and exploration. Brief. Bioinform. 2013, 14, 178–192. [CrossRef] [PubMed]
    • (2013) Brief. Bioinform , vol.14 , pp. 178-192
    • Thorvaldsdóttir, H.1    Robinson, J.T.2    Mesirov, J.P.3
  • 57
    • 84862506964 scopus 로고    scopus 로고
    • A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3
    • CrossRefPubMed
    • Cingolani, P.; Platts, A.; Wang, L.L.; Coon, M.; Nguyen, T.; Wang, L.; Land, S.J.; Lu, X.; Ruden, D.M. A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3. Fly 2012, 6, 80–92. [CrossRef] [PubMed]
    • (2012) Fly , vol.6 , pp. 80-92
    • Cingolani, P.1    Platts, A.2    Wang, L.L.3    Coon, M.4    Nguyen, T.5    Wang, L.6    Land, S.J.7    Lu, X.8    Ruden, D.M.9
  • 58
    • 84949474555 scopus 로고    scopus 로고
    • Available online, accessed on 28 February 2015
    • PolyPhen-2 prediction of functional effects of human nsSNPs. Available online: http://genetics.bwh.harvard.edu/pph2/ (accessed on 28 February 2015).
  • 59
    • 84949460567 scopus 로고    scopus 로고
    • Available online, accessed on 28 February 2015
    • SIFT Blink. Available online: http://sift.jcvi.org/ (accessed on 28 February 2015).
  • 60
    • 84949463208 scopus 로고    scopus 로고
    • accessed on 28 February 2015
    • 1000Genomes. Available online: http://www.1000genomes.org/ (accessed on 28 February 2015).
  • 61
    • 84940892515 scopus 로고    scopus 로고
    • Afatinib resistance in non-small cell lung cancer involves the PI3K/AKT and MAPK/ERK signalling pathways and epithelial-to-mesenchymal transition
    • CrossRefPubMed
    • Coco, S.; Truini, A.; Alama, A.; dal-Bello, M.G.; Venè, R.; Garuti, A.; Carminati, E.; Rijavec, E.; Genova, C.; Barletta, G.; et al. Afatinib resistance in non-small cell lung cancer involves the PI3K/AKT and MAPK/ERK signalling pathways and epithelial-to-mesenchymal transition. Target Oncol. 2015, 10, 393–404. [CrossRef] [PubMed]
    • (2015) Target Oncol , vol.10 , pp. 393-404
    • Coco, S.1    Truini, A.2    Alama, A.3    Dal-Bello, M.G.4    Venè, R.5    Garuti, A.6    Carminati, E.7    Rijavec, E.8    Genova, C.9    Barletta, G.10


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