Electroosmotic perfusion of tissue: Sampling the extracellular space and quantitative assessment of membrane-bound enzyme activity in organotypic hippocampal slice cultures

Analytical and Bioanalytical Chemistry

Volumn 406, Issue 26, 2014, Pages 6455-6468

  Ou, Yangguang ^a   Wu, Juanfang ^a   Sandberg, Mats ^b   Weber, Stephen G ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Electroosmotic sampling; Enzyme activity; Membrane bound enzymes; Neuropeptides; Organotypic hippocampal slice cultures; Thiols

Indexed keywords

BODY FLUIDS; BRAIN; ELECTROOSMOSIS; ENZYME KINETICS; FUSED SILICA; PEPTIDES; TISSUE;

ELECTROOSMOTIC; MEMBRANE-BOUND ENZYMES; NEUROPEPTIDES; ORGANOTYPIC HIPPOCAMPAL SLICE CULTURES; THIOLS;

ENZYME ACTIVITY;

RATTUS;

COENZYME A; MEMBRANE PROTEIN; NEUROPEPTIDE;

ANIMAL; DEVICES; ELECTROOSMOSIS; ENZYMOLOGY; EQUIPMENT DESIGN; EXTRACELLULAR SPACE; HIPPOCAMPUS; HUMAN; METABOLISM; PERFUSION;

ANIMALS; COENZYME A; ELECTROOSMOSIS; EQUIPMENT DESIGN; EXTRACELLULAR SPACE; HIPPOCAMPUS; HUMANS; MEMBRANE PROTEINS; NEUROPEPTIDES; PERFUSION;

EID: 84949116727     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-014-8067-2     Document Type: Review

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