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Volumn 14, Issue 1, 2003, Pages 29-34

Single-cell electroporation

Author keywords

[No Author keywords available]

Indexed keywords

CARBON FIBER; ELECTROLYTE;

EID: 0037309122     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(02)00003-4     Document Type: Review
Times cited : (115)

References (22)
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    • Electroporation of single cells and tissues with an electrolyte-filled capillary
    • This paper describes a novel technique for electroporation with an electrolyte-filled fused silica capillary. Electroporation of small populations of cells, single cells, cellular processes, organotypic tissues and rat brain in vivo are demonstrated.
    • Nolkrantz K., Farre C., Brederlau A., Karlsson R.I., Brennan C., Eriksson P.S., Weber S.G., Sandberg M., Orwar O. Electroporation of single cells and tissues with an electrolyte-filled capillary. Anal. Chem. 73:2001;4469-4477 This paper describes a novel technique for electroporation with an electrolyte-filled fused silica capillary. Electroporation of small populations of cells, single cells, cellular processes, organotypic tissues and rat brain in vivo are demonstrated.
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    • Nolkrantz, K.1    Farre, C.2    Brederlau, A.3    Karlsson, R.I.4    Brennan, C.5    Eriksson, P.S.6    Weber, S.G.7    Sandberg, M.8    Orwar, O.9
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    • Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation
    • This study demonstrates how the electrolyte-filled capillary (EFC) electroporation technique can be used for intracellular protein detection, and proposes electroporation in a multiplex format.
    • Nolkrantz K., Farre C., Hurtig K.J., Rylander P., Orwar O. Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation. Anal. Chem. 74:2002;4300-4305 This study demonstrates how the electrolyte-filled capillary (EFC) electroporation technique can be used for intracellular protein detection, and proposes electroporation in a multiplex format.
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    • Nolkrantz, K.1    Farre, C.2    Hurtig, K.J.3    Rylander, P.4    Orwar, O.5
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    • Single-cell electroporation for gene transfer in vivo
    • This article demonstrates electroporation with a micropipette filled with cell-loading agents into single cells in tissue slices and in vivo.
    • Haas K., Sin W.C., Javaherian A., Li Z., Cline H.T. Single-cell electroporation for gene transfer in vivo. Neuron. 29:2001;583-591 This article demonstrates electroporation with a micropipette filled with cell-loading agents into single cells in tissue slices and in vivo.
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    • Haas, K.1    Sin, W.C.2    Javaherian, A.3    Li, Z.4    Cline, H.T.5
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    • Single-cell electroporation
    • The authors describe the use of a modified patch-clamp technique for electroporation of single cells with a high transfection rate.
    • Rae J.L., Levis R.A. Single-cell electroporation. Pflugers Arch. 443:2002;664-670 The authors describe the use of a modified patch-clamp technique for electroporation of single cells with a high transfection rate.
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    • Rae, J.L.1    Levis, R.A.2
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    • Micro-electroporation: Improving the efficiency and understanding of electrical permeabilisation of cells
    • Demonstration of a chip-based system for single-cell electroporation in fluidic systems. Chip fabrication and preliminary results are shown.
    • Huang Y., Rubinsky B. Micro-electroporation: improving the efficiency and understanding of electrical permeabilisation of cells. Biomed. Microdev. 3:2000;145-150 Demonstration of a chip-based system for single-cell electroporation in fluidic systems. Chip fabrication and preliminary results are shown.
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    • Huang, Y.1    Rubinsky, B.2
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    • Selective introduction of antisense oligonucleotides in single adult CNS progenitor cells using electroporation demonstrates that CNTF-induced gliogenesis requires STST3 activation
    • This paper uses the method developed by Lundqvist et al. [7] for electroporation of single adult progenitor cells to internalize antisense oligonucleotides as a way of characterizing a signaling pathway in gliogenesis.
    • Åberg M.A.I., Ryttsén F., Hellgren G., Lindell K., Rosengren L.E., MacLennan A.J., Carlsson B., Orwar O., Eriksson P.S. Selective introduction of antisense oligonucleotides in single adult CNS progenitor cells using electroporation demonstrates that CNTF-induced gliogenesis requires STST3 activation. Mol. Cell. Neurosci. 17:2001;426-443 This paper uses the method developed by Lundqvist et al. [7] for electroporation of single adult progenitor cells to internalize antisense oligonucleotides as a way of characterizing a signaling pathway in gliogenesis.
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    • Åberg, M.A.I.1    Ryttsén, F.2    Hellgren, G.3    Lindell, K.4    Rosengren, L.E.5    MacLennan, A.J.6    Carlsson, B.7    Orwar, O.8    Eriksson, P.S.9
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    • Characterization of single-cell electroporation by using patch clamp and fluorescence microscopy
    • In this report, the electroporation method by Lundqvist et al. [7] is characterized regarding threshold potential for pore formation, kinetics for pore opening and closure, and how the electric field is distributed around the electroporated cell.
    • Ryttsén F., Farre C., Brennan C., Weber S.G., Nolkrantz K., Jardemark K., Chiu D.T., Orwar O. Characterization of single-cell electroporation by using patch clamp and fluorescence microscopy. Biophys J. 79:2000;1993-2001 In this report, the electroporation method by Lundqvist et al. [7] is characterized regarding threshold potential for pore formation, kinetics for pore opening and closure, and how the electric field is distributed around the electroporated cell.
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    • Electroinjection of colloid particles and biopolymers into single unilamellar liposomes and cells for bioanalytical applications
    • In this paper a method using both mechanical and electrical forces to perform permeabilisation of bilayer lipid membrane is presented. The technique allows introduction of larger size compounds to smaller cells relative to microinjection.
    • Karlsson M., Nolkrantz K., Davidson M.J., Strömberg A., Ryttsén F., Åkerman B., Orwar O. Electroinjection of colloid particles and biopolymers into single unilamellar liposomes and cells for bioanalytical applications. Anal. Chem. 72:2000;5857-5862 In this paper a method using both mechanical and electrical forces to perform permeabilisation of bilayer lipid membrane is presented. The technique allows introduction of larger size compounds to smaller cells relative to microinjection.
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    • Karlsson, M.1    Nolkrantz, K.2    Davidson, M.J.3    Strömberg, A.4    Ryttsén, F.5    Åkerman, B.6    Orwar, O.7
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    • A microfluidics platform for cell fusion
    • A recent review on the subject of cell fusion in fluidic systems applicable for electroporation as well.
    • Chiu D.T. A microfluidics platform for cell fusion. Curr. Opin. Chem. Biol. 5:2001;609-612 A recent review on the subject of cell fusion in fluidic systems applicable for electroporation as well.
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    • Chiu, D.T.1
  • 19
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    • Electroporation microchips for continuous gene transfection
    • A partly chip-implemented setup for continuous electroporation and gene transfection is presented in this article. Cells in suspension are flown through an electroporating electric field on a microchip.
    • Lin Y.-C., Jen C.-M., Huang M.-Y., Wu C.-Y., Lin X.-Z. Electroporation microchips for continuous gene transfection. Sensor Actuat. B. 79:2001;137-143 A partly chip-implemented setup for continuous electroporation and gene transfection is presented in this article. Cells in suspension are flown through an electroporating electric field on a microchip.
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    • Lin, Y.-C.1    Jen, C.-M.2    Huang, M.-Y.3    Wu, C.-Y.4    Lin, X.-Z.5
  • 20
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    • Electroporation microchips for in vitro gene transfection
    • This article discloses a microchip for in vitro gene transfection of small populations of cells.
    • Lin Y.-C., Huang M.-Y. Electroporation microchips for in vitro gene transfection. J. Micromech. Microeng. 11:2001;542-547 This article discloses a microchip for in vitro gene transfection of small populations of cells.
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    • Lin, Y.-C.1    Huang, M.-Y.2
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    • Voltage-induced pore formation and hemolysis of human erythrocytes
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    • Kinosita, K.1    Tsong, T.Y.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.