Sequencing of plasmids pAMBL1 and pAMBL2 from Pseudomonas aeruginosa reveals a blaVIM-1 amplification causing high-level carbapenem resistance

Journal of Antimicrobial Chemotherapy

Volumn 70, Issue 11, 2015, Pages 3000-3003

  San Millan, Alvaro ^a   Toll Riera, Macarena ^a,f   Escudero, Jose Antonio ^b   Cantón, Rafael ^c,d   Coque, Teresa M ^c,d,e   Craig MacLean, R ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b CNRS   (France)

^c HOSPITAL RAMÓN Y CAJAL   (Spain)

^d CSIC   (Spain)

^e CIBER EPIDEMIOLOGÍA Y SALUD PÚBLICA CIBERESP   (Spain)

^f UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CARBAPENEM; CARBAPENEMASE; METALLO BETA LACTAMASE; UNCLASSIFIED DRUG; VIM 1 METALLO BETA LACTAMASE; ANTIINFECTIVE AGENT; BACTERIAL DNA; BETA LACTAMASE; CARBAPENEM DERIVATIVE; VIM-1 METALLO-BETA-LACTAMASE;

ACHROMOBACTER XYLOSOXIDANS; ANTIBIOTIC RESISTANCE; ARTICLE; INTEGRON; NEXT GENERATION SEQUENCING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS PUTIDA; ACHROMOBACTER DENITRIFICANS; CHEMISTRY; DNA SEQUENCE; DRUG EFFECTS; GENE DOSAGE; GENETICS; HUMAN; MOLECULAR GENETICS; SECRETION (PROCESS); SEQUENCE HOMOLOGY;

ACHROMOBACTER DENITRIFICANS; ANTI-BACTERIAL AGENTS; BETA-LACTAM RESISTANCE; BETA-LACTAMASES; CARBAPENEMS; DNA, BACTERIAL; GENE DOSAGE; HUMANS; MOLECULAR SEQUENCE DATA; PLASMIDS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS PUTIDA; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY;

EID: 84949034613     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkv222     Document Type: Article

References (24)

1. Lister PD, Wolter DJ, Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev 2009; 22: 582-610.
2. Shintani M, Sanchez ZK, Kimbara K. Genomics of microbial plasmids: classification and identification based on replication and transfer systems and host taxonomy. Front Microbiol 2015; 6: 242.
3. Tato M, Coque TM, Baquero F et al. Dispersal of carbapenemase blaVIM-1 gene associated with different Tn402 variants, mercury transposons, and conjugative plasmids in Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob Agents Chemother 2010; 54: 320-7.
4. San Millan A, Heilbron K, MacLean RC. Positive epistasis between co-infecting plasmids promotes plasmid survival in bacterial populations. ISME J 2014; 8: 601-12.
5. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Nineteenth Informational Supplement M100-S19. CLSI, Wayne, PA, USA, 2009.
6. Tritt A, Eisen JA, Facciotti MT et al. An integrated pipeline for de novo assembly of microbial genomes. PLoS One 2012; 7: e42304.
7. Aziz RK, Bartels D, Best AA et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 2008; 9: 75.
8. San Millan A, Toll-Riera M, Qi Q et al. Interactions between horizontally acquired genes create a fitness cost in Pseudomonas aeruginosa. Nat Commun 2015; 6: 6845.
9. Alkan C, Sajjadian S, Eichler EE. Limitations of next-generation genome sequence assembly. Nat Methods 2011; 8: 61-5.
10. Bonnin RA, Poirel L, Nordmann P et al. Complete sequence of broadhost-range plasmid pNOR-2000 harbouring the metallo-ß-lactamase gene blaVIM-2 from Pseudomonas aeruginosa. J Antimicrob Chemother 2013; 68: 1060-5.
11. Di Pilato V, Pollini S, Rossolini GM. Characterization of plasmid pAX22, encoding VIM-1 metallo-ß-lactamase, reveals a new putative mechanism of In70 integron mobilization. J Antimicrob Chemother 2014; 69: 67-71.
12. Klockgether J, Reva O, Larbig K et al. Sequence analysis of the mobile genome island pKLC102 of Pseudomonas aeruginosa C. J Bacteriol 2004; 186: 518-34.
13. Szuplewska M, Ludwiczak M, Lyzwa K et al. Mobility and generation of mosaic non-autonomous transposons by Tn3-derived inverted-repeat miniature elements (TIMEs). PLoS One 2014; 9: e105010.
14. Molina L, Udaondo Z, Duque E et al. Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital. PLoS One 2014; 9: e81604.
15. Bramucci M, Chen M, Nagarajan V. Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol 2006; 71: 67-74.
16. Arcus VL, McKenzie JL, Robson J et al. The PIN-domain ribonucleases and the prokaryotic VapBC toxin-antitoxin array. Protein Eng Des Sel 2011; 24: 33-40.
17. Jové T, Da Re S, Denis F et al. Inverse correlation between promoter strength and excision activity in class 1 integrons. PLoS Genet 2010; 6: e1000793.
18. O'Callaghan CH, Morris A, Kirby SM et al. Novel method for detection of ß-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother 1972; 1: 283-8.
19. Tato M, Morosini M, García L et al. Carbapenem heteroresistance in VIM-1-producing Klebsiella pneumoniae isolates belonging to the same clone: consequences for routine susceptibility testing. J Clin Microbiol 2010; 48: 4089-93.
20. Stokes HW, Hall RM. The integron In1 in plasmid R46 includes two copies of the oxa2 gene cassette. Plasmid 1992; 28: 225-34.
21. Loot C, Ducos-Galand M, Escudero JA et al. Replicative resolution of integron cassette insertion. Nucleic Acids Res 2012; 40: 8361-70.
22. Escudero JA, Loot C, Nivina A et al. The integron: adaptation on demand. Microbiol Spectr 2015; 3: doi:10.1128/microbiolspec.MDNA3-0019-2014.
23. Taylor JS, Raes J. Duplication and divergence: the evolution of new genes and old ideas. Annu Rev Genet 2004; 38: 615-43.
24. Moura A, Soares M, Pereira C et al. INTEGRALL: a database and search engine for integrons, integrases and gene cassettes. Bioinformatics 2009; 25: 1096-8.