Carbapenem heteroresistance in VIM-1-producing Klebsiella pneumoniae isolates belonging to the same clone: Consequences for routine susceptibility testing

Journal of Clinical Microbiology

Volumn 48, Issue 11, 2010, Pages 4089-4093

  Tato, M ^a   Morosini, M ^a   Garcia L ^c   Alberti S ^c   Coque, M T ^a,b   Canton R ^a,b  

^a HOSPITAL RAMÓN Y CAJAL   (Spain)

^b CSIC   (Spain)

^c UNIVERSITAT DE LES ILLES BALEARS   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CARBAPENEM; ERTAPENEM; IMIPENEM; MEROPENEM; PORIN;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM ISOLATE; BROTH DILUTION; HUMAN; KLEBSIELLA PNEUMONIAE; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR CLONING; MULTIDRUG RESISTANCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPRODUCIBILITY;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; BETA-LACTAMASES; CARBAPENEMS; DNA FINGERPRINTING; GENETIC VARIATION; GENOTYPE; HUMANS; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; MICROBIAL SENSITIVITY TESTS; PHENOTYPE;

ENTEROBACTERIACEAE; KLEBSIELLA PNEUMONIAE;

EID: 78049509132     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01130-10     Document Type: Article

References (28)

1. Cantón, R., M. Pérez-Vázquez, A. Oliver, B. Sánchez Del Saz, M. O. Gutiérrez, M. Martínez-Ferrer, and F. Baquero. 2000. Evaluation of the Wider system, a new computer-assisted image-processing device for bacterial identification and susceptibility testing. J. Clin. Microbiol. 38:1339-1346.
2. Carmeli, Y., M. Akova, G. Cornaglia, G. L. Daikos, J. Garau, S. Harbarth, G. M. Rossolini, M. Souli, and H. Giamarellou. 2010. Controlling the spread of carbapenemase-producing Gram-negatives: therapeutic approach and infection control. Clin. Microbiol. Infect. 16:102-111.
3. Clinical and Laboratory Standards Institute. 2010. Performance standards for antimicrobial susceptibility testing: 20th international Supplement. M100-S20. CLSI, Wayne, PA.
4. Clinical and Laboratory Standards Institute. 2010. Performance standards for antimicrobial susceptibility testing: 20th international supplement. M100-S20. June update. CLSI, Wayne, PA.
5. Collis, C. M., and R. M. Hall. 1995. Expression of antibiotic resistance genes in the integrated cassettes of integrons. Antimicrob. Agents Chemother. 39:155-162.
6. Cornaglia, G., M. Akova, G. Amicosante, R. Cantón, R. Cauda, J. D. Docquier, M. Edelstein, J. M. Frère, M. Fuzi, M. Galleni, H. Giamarellou, M. Gniadkowski, R. Koncan, B. Libisch, F. Luzzaro, V. Miriagou, F. Navarro, P. Nordmann, L. Pagani, L. Peixe, L. Poirel, M. Souli, E. Tacconelli, A. Vatopoulos, and G. M. Rossolini; ESCMID Study Group for Antimicrobial Resistance Surveillance (ESGARS). 2007. Metallo-beta-lactamases as emerging resistant determinants in Gram-negative pathogens: open issues. Int. J. Antimicrob. Agents 29:380-388.
7. Daikos, G. L., P. Petrikkos, M. Psichogiou, C. Kosmidis, E. Vryonis, A. Skoutelis, K. Georgousi, L. S. Tzouvelekis, P. T. Tassios, C. Bamia, and G. Petrikkos. 2009. Prospective observational study of the impact of VIM-1 metallo-β-lactamase on the outcome of patients with Klebsiella pneumoniae bloodstream infections. Antimicrob. Agents Chemother. 53:1868-1873.
8. Falcone, M., M. L. Mezzatesta, M. Perilli, C. Forcella, A. Giordano, V. Cafiso, G. Amicosante, S. Stefani, and M. Venditti. 2009. Infections with VIM-1 metallo-β-lactamase-producing Enterobacter cloacae and their correlation with clinical outcome. J. Clin. Microbiol. 47:3514-3519.
9. Franklin, C., L. Liolios, and A. Y. Peleg. 2006. Phenotypic detection of carbapenem-susceptible metallo-beta-lactamase-producing gram-negative bacilli in the clinical laboratory. J. Clin. Microbiol. 44:3139-3144.
10. Giakkoupi, P., L. S. Tzouvelekis, G. L. Daikos, V. Miriagou, G. Petrikkos, N. J. Legakis, and A. C. Vatopoulos. 2005. Discrepancies and interpretation problems in susceptibility testing of VIM-1-producing Klebsiella pneumoniae isolates. J. Clin. Microbiol. 43:494-496.
11. Gordon, N. C., and D. W. Wareham. 2009. Failure of the MicroScan Walk-Away System to detect heteroresistance to carbapenems in a patient with Enterobacter aerogenes bacteremia. J. Clin. Microbiol. 47:3024-3025.
12. Ikonomidis, A., E. Neou, V. Gogou, G. Vrioni, A. Tsakris, and S. Pournaras. 2009. Heteroresistance to meropenem in carbapenem-susceptible Acinetobacter baumanii. J. Clin. Microbiol. 47:4055-4059.
13. Kitchel, B., J. K. Rasheed, A. Endimiani, A. M. Hujer, K. F. Anderson, R. A. Bonomo, and J. B. Patel. 26 July 2010. Genetic factors associated with elevated carbapenem resistance in KPC-producing Klebsiella pneumoniae. Antimicrob. Agents Chemother. [Epub ahead of print.] doi:10.1128/AAC.00008-10.
14. Lévesque, C., S. Brassard, and P. H. Roy. 1994. Diversity and relative strength of tandem promoters for the antibiotic-resistance genes of several integrons. Gene 142:49-54.
15. Loli, A., L. S. Tzouvelekis, E. Tzelepi, A. Carattoli, A. C. Vatopoulos, P. T. Tassios, and V. Miriagou. 2006. Sources of diversity of carbapenem resistance levels in Klebsiella pneumoniae carrying blaVIM-1. J. Antimicrob. Chemother. 58:669-672. (Pubitemid 44288137)
16. Martínez-Martínez, L., A. Pascual, S. Hernández- Allés, D. Alvarez-Díaz, A. I. Suárez, J. Tran, V. J. Benedí, and G. A. Jacoby. 1999. Roles of β-lactamases and porins in activities of carbapenems and cephalosporins against Klebsiella pneumoniae. Antimicrob. Agents Chemother. 43:1669-1673.
17. Mena, A., V. Plasencia, L. García, O. Hidalgo, J. I. Ayestarán, S. Alberti, N. Borrell, J. L. Pérez, and A. Oliver. 2006. Characterization of a large outbreak by CTX-M-1-producing Klebsiella pneumoniae and mechanisms leading to in vivo carbapenem resistance development. J. Clin. Microbiol. 44:2831-2837.
18. Miriagou, V., G. Cornaglia, M. Edelstein, I. Galani, C. G. Giske, M. Gniadkowski, E. Malamou-Lada, L. Martinez-Martinez, F. Navarro, P. Nordmann, L. Peixe, S. Pournaras, G. M. Rossolini, A. Tsakris, A. Vatopoulos, and R. Cantón. 2010. Acquired carbapenemases in gram-negative bacterial pathogens: detection and surveillance issues. Clin. Microbiol. Infect. 16:112-122.
19. Pournaras, S., A. Ikonomidis, A. Markogiannakis, A. N. Maniatis, and A. Tsakris. 2005. Heteroresistance to carbapenems in Acinetobacter baumannii. J. Antimicrob. Chemother. 55:1055-1056.
20. Pournaras, S., A. Ikonomidis, A. Markogiannakis, N. Spanakis, A. N. Maniatis, and A. Tsakris. 2007. Characterization of clinical isolates of Pseudomonas aeruginosa heterogeneously resistant to carbapenems. J. Med. Microbiol. 56:66-70.
21. Pournaras, S., I. Kristo, G. Vrioni, A. Ikonomidis, A. Poulou, D. Petropoulou, and A. Tsakris. 2010. Characteristics of meropenem heteroresistance in Klebsiella pneumoniae carbapenemase (KPC)-producing clinical isolates of K. pneumoniae. J. Clin. Microbiol. 48:2601-2604.
22. Rinder, H. 2001. Hetero-resistance: an underrecognised confounder in diagnosis and therapy? J. Med. Microbiol. 50:1018-1020.
23. Sakoulas, G., and R. C. Moellering, Jr. 2008. Increasing antibiotic resistance among methicillin-resistant Staphylococcus aureus strains. Clin. Infect. Dis. 46:S360-367.
24. Savini, V., M. Favaro, C. Fontana, C. Catavitello, A. Balbinot, M. Talia, F. Febbo, and D. D'Antonio. 2009. Bacillus cereus heteroresistance to carbapenems in a cancer patient. J. Hosp. Infect. 71:288-290.
25. Tato, M., T. M. Coque, P. Ruíz-Garbajosa, V. Pintado, J. Cobo, H. S. Sader, R. N. Jones, F. Baquero, and R. Cantón. 2007. Complex clonal and plasmid epidemiology in the first outbreak of Enterobacteriaceae infection involving VIM-1 metallo-beta-lactamase in Spain: toward endemicity? Clin. Infect. Dis. 45:1171-1178.
26. Tato, M., T. M. Coque, F. Baquero, and R. Cantón. 2010. Dispersal of carbapenemase blaVIM-1 gene associated with different Tn402 variants, mercury transposons, and conjugative plasmids in Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 54:320-327.
27. Tenover, F. C., R. D. Arbeit, R. V. Goering, P. A. Mickelsen, B. E. Murray, D. H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33:2233-2239.
28. Tenover, F. C., R. K. Kalsi, P. P. Williams, R. B. Carey, S. Stocker, D. Lonsway, J. K. Rasheed, J. W. Biddle, J. E. McGowan, Jr., and B. Hanna. 2006. Carbapenem resistance in Klebsiella pneumoniae not detected by automated susceptibility testing. Emerg. Infect. Dis. 12:1209-1213.