Process for Assembly and Transformation into Saccharomyces cerevisiae of a Synthetic Yeast Artificial Chromosome Containing a Multigene Cassette to Express Enzymes That Enhance Xylose Utilization Designed for an Automated Platform

Journal of Laboratory Automation

Volumn 20, Issue 6, 2015, Pages 621-635

  Hughes, Stephen R ^a   Cox, Elby J ^a,b   Bang, Sookie S ^c   Pinkelman, Rebecca J ^c   López Núñez, Juan Carlos ^d   Saha, Badal C ^a   Qureshi, Nasib ^a   Gibbons, William R ^e   Fry, Michelle R ^b   Moser, Bryan R ^a   Bischoff, Kenneth M ^a   Liu, Siqing ^a   Sterner, David E ^f   Butt, Tauseef R ^g   Riedmuller, Steven B ^h   Jones, Marjorie A ⁱ   Riaño Herrera, Néstor M ^d  

^a NATIONAL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH   (United States)

^b BRADLEY UNIVERSITY   (United States)

^c South Dakota School of Mines and Technology   (United States)

^d Cenicafé FNC National Center of Coffee Research National Federation of Coffee Growers   (Colombia)

^e SOUTH DAKOTA STATE UNIVERSITY   (United States)

^f Progenra Inc   (United States)

^g LifeSensors Inc   (United States)

^h Hudson Control Group Inc   (United States)

ⁱ ILLINOIS STATE UNIVERSITY   (United States)

Author keywords

multigene expression cassette; stable cellulosic yeast; synthetic biology platform; synthetic yeast chromosome assembly; yeast artificial expression chromosome

Indexed keywords

AUTOMATION; CHROMOSOMES; ENZYMES; ETHANOL; GENE EXPRESSION; GLUCOSE; XYLOSE;

AUTOMATED SYNTHESIS; EXPRESSION CASSETTE; GLUCOSE FERMENTATION; PROTEIN EXPRESSION SYSTEMS; SPENT COFFEE GROUNDS; SYNTHETIC BIOLOGY; XYLOSE UTILIZATIONS; YEAST ARTIFICIAL CHROMOSOMES;

YEAST;

XYLOSE; ALCOHOL; COFFEE; CULTURE MEDIUM; ENZYME;

ARTICLE; GENE CASSETTE; GENE CONTROL; IMMUNODETECTION; LABORATORY AUTOMATION; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; SCANNING ELECTRON MICROSCOPY; WESTERN BLOTTING; YEAST ARTIFICIAL CHROMOSOME; CHEMISTRY; COFFEE; CULTURE MEDIUM; FERMENTATION; GENE EXPRESSION; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; MAIZE; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

CHROMOSOMES, ARTIFICIAL, YEAST; COFFEE; CULTURE MEDIA; ENZYMES; ETHANOL; FERMENTATION; GENE EXPRESSION; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC; XYLOSE; ZEA MAYS;

EID: 84948679632     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1177/2211068215573188     Document Type: Article

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