Serum sample containing endogenous antibodies interfering with multiple hormone immunoassays. Laboratory strategies to detect interference

Practical Laboratory Medicine

Volumn 4, Issue , 2016, Pages 1-10

  García González, Elena ^a   Aramendía, Maite ^b   Álvarez Ballano, Diego ^a   Trincado, Pablo ^a   Rello, Luis ^a  

^a HOSPITAL UNIVERSITARIO MIGUEL SERVET   (Spain)

^b CENTRO UNIVERSITARIO DE LA DEFENSA   (Spain)

Author keywords

Case report; Endogenous antibodies; Immunoassay; Interference; Pituitary hormones

Indexed keywords

ANTIBODY; CORTICOTROPIN; FOLLITROPIN; LEVOTHYROXINE; LUTEINIZING HORMONE; MACROGOL; PROLACTIN; THYROTROPIN;

ADDISON DISEASE; ADULT; ARTICLE; ASYMPTOMATIC DISEASE; CASE REPORT; CONTROLLED STUDY; CORTICOTROPIN BLOOD LEVEL; CUSHING DISEASE; DIAGNOSTIC ERROR; DIARRHEA; DILUTION; DRUG DOSE INCREASE; FOLLITROPIN BLOOD LEVEL; HEADACHE; HEART PALPITATION; HORMONE DETERMINATION; HUMAN; HYPOPHYSIS ADENOMA; IMMUNOASSAY; INSOMNIA; LABORATORY DIAGNOSIS; LUTEINIZING HORMONE BLOOD LEVEL; MALE; PRECIPITATION; PROLACTIN BLOOD LEVEL; SIDE EFFECT; SUBCLINICAL HYPOTHYROIDISM; THYROID FUNCTION TEST; THYROTROPIN BLOOD LEVEL; WEIGHT REDUCTION;

EID: 84948656251     PISSN: None     EISSN: 23525517     Source Type: Journal    
DOI: 10.1016/j.plabm.2015.11.001     Document Type: Article

References (23)

1. Bolstad N., Warren D.J., Nustad K. Heterophilic antibody interference in immunometric assays. Best Pract. Res.: Clin. Endocrinol. Metab. 2013, 27:647-661.
2. Levinson S.S., Miller J.J. Towards a better understanding of heterophile (and the like) antibody interference with modern immunoassays. Clin. Chim. Acta 2002, 325:1-15.
3. Emerson J.F., Lai K.K.Y. Endogenous antibody interferences in immunoassays. Lab. Med. 2013, 44:69-73.
4. Jones A.M., Honour J.W. Unusual results from immunoassays and the role of the clinical endocrinologist. Clin. Endocrinol. 2006, 64:234-244.
5. Després N., Grant A.M. Antibody interference in thyroid assays: a potential for clinical misinformation. Clin. Chem. 1998, 44:440-454.
6. Ismail A.A.A., Walker P.L., Barth J.H., Lewandowski K.C., Jones R., Burr W.A. Wrong biochemistry results: two case reports and observational study in 5310 patients on potentially misleading thyroid-stimulating hormone and gonadotropin immunoassay results. Clin. Chem. 2002, 48:2023-2029.
7. Papapetrou P.D., Polymeris A., Karga H., Vaiopoulos G. Heterophilic antibodies causing falsely high serum calcitonin values. J. Endocrinol. Investig. 2006, 29:919-923.
8. Lippi G., Aloe R., Meschi T., Borghi L., Cervellin G. Interference from heterophilic antibodies in troponin testing. Case report and systematic review of the literature. Clin. Chim. Acta 2013, 426:79-84.
9. Marks V. False-positive immunoassay results: a multicenter survey of erroneous immunoassay results from assays of 74 analytes in 10 donors from 66 laboratories in seven countries. Clin. Chem. 2002, 48:2008-2016.
10. Fryer A.A., Smellie W.S.A. Managing demand for laboratory tests: a laboratory toolkit. J. Clin. Pathol. 2012, 66:62-72.
11. Ismail A.A.A. Interference from endogenous antibodies in automated immunoassays: what laboratorians need to know. J. Clin. Pathol. 2009, 62:673-678.
12. Tate J., Ward G. Interferences in immunoassay. Clin. Biochem. Rev. 2004, 25:105-120.
13. Sturgeon C.M., Viljoen A. Analytical error and interference in immunoassay: minimizing risk. Ann. Clin. Biochem. 2011, 48:418-432.
14. Gulbahar O., Degertekin K.C., Akturk M., Yalcin M.M., Kalan I., Atikeler G.F., et al. A case with immunoassay interferences in the measurement of multiple hormones. J. Clin. Endocrinol. Metab. 2015, 100:2147-2153.
15. Ismail A.A.A. On the diagnosis of subclinical hypothyroidism. Br. J. Gen. Pract. 2007, 57:1000-1001.
16. Ismail A., Burr W.A., Walker P.L. Acute changes in serum thyrotrophin in treated Addison's disease. Clin. Endocrinol. 1989, 30:225-230.
17. Bjerner J., Bormer O.P., Nustad K. The war on heterophilic antibody interference. Clin. Chem. 2005, 51:9-11.
18. Ismail A.A.A. On detecting interference from endogenous antibodies in immunoassays by doubling dilutions test. Clin. Chem. Lab. Med. 2007, 45:851-854.
19. Jassam N.F., Paterson A., Lippiatt C., Barth J.H. Macroprolactin on the Advia Centaur: experience with 409 patients over a three-year period. Ann. Clin. Biochem. 2009, 46:501-504.
20. Ellis M.J., Livesey J.H. Techniques for identifying heterophile antibody interference are assay specific: study of seven analytes on two automated immunoassay analyzers. Clin. Chem. 2005, 51:639-641.
21. Fahie-Wilson M., Halsall D. Polyethylene glycol precipitation: proceed with care. Ann. Clin. Biochem. 2008, 45:233-235.
22. Bolstad N., Kazaryan A.M., Revheim M.E., Distante S., Johnsrud K., Warren D.J., et al. A man with abdominal pain: enough evidence for surgery?. Clin. Chem. 2012, 58:1187-1190.
23. Ismail A.A.A., Ismail A.A., Ismail Y. Probabilistic Bayesian reasoning can help identifying potentially wrong immunoassays results in clinical practice: even when they appear "not-unreasonable". Ann. Clin. Biochem. 2011, 48:65-71.