Production of farnesene and santalene by Saccharomyces cerevisiae using fed-batch cultivations with RQ-controlled feed

Biotechnology and Bioengineering

Volumn 113, Issue 1, 2016, Pages 72-81

  Tippmann, Stefan ^a   Scalcinati, Gionata ^a,c   Siewers, Verena ^a   Nielsen, Jens ^a,b  

^a CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

^b Chalmers University of Technology   (Denmark)

^c GENOMATICA INC   (United States)

Author keywords

Biofuels; Fed batch; Isoprenoids; Metabolic engineering; Terpene synthases; Yeast

Indexed keywords

BIOFUELS; ESCHERICHIA COLI; FERMENTATION; METABOLIC ENGINEERING; METABOLISM; TERPENES;

CATALYTIC EFFICIENCIES; FARNESYL DIPHOSPHATE; FED BATCHES; FED-BATCH CULTIVATION; FED-BATCH FERMENTATION; ISOPRENOIDS; SHAKE FLASK CULTIVATIONS; SYNTHASES;

YEAST;

ALPHA FARNESENE SYNTHASE; FARNESENE; SANTALENE; SESQUITERPENE; TERPENE; UNCLASSIFIED DRUG; PLANT PROTEIN; RECOMBINANT PROTEIN; TERPENE SYNTHASE; TRANSFERASE;

ARTICLE; BIOMASS PRODUCTION; DOWN REGULATION; ENZYME ACTIVITY; FED BATCH FERMENTATION; FUNGAL METABOLISM; FUNGAL STRAIN; GROWTH; MALUS DOMESTICA; NONHUMAN; SACCHAROMYCES CEREVISIAE; SPECIES CULTIVATION; BIOREACTOR; CLAUSENA; ENZYMOLOGY; GENETICS; MALUS; METABOLISM; MICROBIOLOGY;

ALKYL AND ARYL TRANSFERASES; BIOREACTORS; CLAUSENA; MALUS; PLANT PROTEINS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SESQUITERPENES;

EID: 84948065021     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25683     Document Type: Article

References (34)

1. Asadollahi MA, Maury J, Møller K, Nielsen KF, Schalk M, Clark A, Nielsen J. 2008. Production of plant sesquiterpenes in Saccharomyces cerevisiae: Effect of ERG9 repression on sesquiterpene biosynthesis. Biotechnol Bioeng 99(3):666-677.
2. Beom Soo K, Seung Chul L, Sang Yup L, Ho Nam C, Yong Keun C, Seong Ihl W. 1994. Production of poly(3-hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control. Biotechnol Bioeng 43(9):892-898.
3. Chen F, Tholl D, Bohlmann J, Pichersky E. 2011. The family of terpene synthases in plants: A mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom. Plant J. 66(1):212-229.
4. Crock J, Wildung M, Croteau R. 1997. Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-β-farnesene. Proc Natl Acad Sci USA 94(24):12833-12838.
5. Ding MZ, Yan HF, Li LF, Zhai F, Shang LQ, Yin Z, Yuan YJ. 2014. Biosynthesis of taxadiene in Saccharomyces cerevisiae: Selection of geranylgeranyl diphosphate synthase directed by a computer-aided docking strategy. PLoS ONE 9(10):1-9.
6. Geurts TGE, De Kok HE, Roels JA. 1980. A quantitative description of the growth of Saccharomyces cerevisiae CBS 426 on a mixed substrate of glucose and ethanol. Biotechnol Bioeng 22(10):2031-2043.
7. Green S, Friel EN, Matich A, Beuning LL, Cooney JM, Rowan DD, MacRae E. 2007. Unusual features of a recombinant apple α-farnesene synthase. Phytochemistry 68(2):176-188.
8. Holm T. 1999. Aspects of the mechanism of the flame ionization detector. J Chromatogr A 842(1-2):221-227.
9. Martin VJJ, Yoshikuni Y, Keasling JD. 2001. The in vivo synthesis of plant sesquiterpenes by Escherichia coli. Biotechnol Bioeng 75(5):497-503.
10. Maruyama T, Ito M, Honda G. 2001. Molecular cloning, functional expression and characterization of (E)-β-farnesene synthase from Citrus junos. Biol Pharm Bull 24(10):1171-1175.
11. Meylemans HA, Quintana RL, Harvey BG. 2012. Efficient conversion of pure and mixed terpene feedstocks to high density fuels. Fuel 97:560-568.
12. Millo F, Bensaid S, Fino D, Castillo Marcana SJ, Vlachos T, Debnath BK. 2014. Influence on the performance and emissions of an automotive Euro 5 diesel engine fueled with F30 from Farnesane. Fuel 138:134-142.
13. Nielsen J, Villadsen J, Lidén G. 2002. Bioreaction engineering principles. Springer Verlag. p. 411-416.
14. Ohto C, Muramatsu M, Obata S, Sakuradani E, Shimizu S. 2009. Overexpression of the gene encoding HMG-CoA reductase in Saccharomyces cerevisiae for production of prenyl alcohols. Appl Microbiol Biotechnol 82(5):837-845.
15. Ozcan S, Johnston M. 1995. Three different regulatory mechanisms enable yeast hexose transporter (HXT) genes to be induced by different levels of glucose. Mol Cell Biol 15(3):1564-1572.
16. Paddon CJ, Westfall PJ, Pitera DJ, Benjamin K, Fisher K, McPhee D, Leavell MD, Tai A, Main A, Eng D. Ellens KW, Fickes S, Galazzo J, Gaucher SP, Geistlinger T, Henry R, Hepp M, Horning T, Iqbal T, Kizer L, Lieu B, Melis D, Moss N, Regentin R, Secrest S, Tsuruta H, Vazquez R, Westblade LF, Xu L, Yu M, Zhang Y, Zhao L, Lievense J, Covello PS, Keasling JD, Reiling KK, Renninger NS, Newman JD. 2013. High-level semi-synthetic production of the potent antimalarial artemisinin. Nature 496(7446):528-532.
17. Partow S, Siewers V, Bjørn S, Nielsen J, Maury J. 2010. Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae. Yeast 27(11):955-964.
18. Pechous SW, Whitaker BD. 2004. Cloning and functional expression of an (E, E)-α-farnesene synthase cDNA from peel tissue of apple fruit. Planta 219(1):84-94.
19. Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS. 2011. Identification and microbial production of a terpene-based advanced biofuel. Nat Commun 2:483.
20. Pham HTB, Larsson G, Enfors SO. 1998. Growth and energy metabolism in aerobic fed-batch cultures of Saccharomyces cerevisiae: Simulation and model verification. Biotechnol Bioeng 60(4):474-482.
21. Picaud S, Brodelius M, Brodelius PE. 2005. Expression, purification and characterization of recombinant (E)-β-farnesene synthase from Artemisia annua. Phytochemistry 66(9):961-967.
22. Polakowski T, Stahl U, Lang C. 1998. Overexpression of a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast. Appl Microbiol Biotechnol 49(1):66-71.
23. Reifenberger E, Freidel K, Ciriacy M. 1995. Identification of novel HXT genes in Saccharomyces cerevisiae reveals the impact of individual hexose transporters on glycolytic flux. Mol Microbiol 16(1):157-167.
24. Rupasinghe HPV, Paliyath G, Murr DP. 2000. Sesquiterpene α-farnesene synthase: Partial purification, characterization, and activity in relation to superficial scald development in apples. J Am Soc Hortic Sci 125(1):111-119.
25. Sarria S, Wong B, Martín HG, Keasling JD, Peralta-Yahya P. 2014. Microbial synthesis of pinene. ACS Synth Biol 3(7):466-475.
26. Scalcinati G, Knuf C, Partow S, Chen Y, Maury J, Schalk M, Daviet L, Nielsen J, Siewers V. 2012a. Dynamic control of gene expression in Saccharomyces cerevisiae engineered for the production of plant sesquitepene α-santalene in a fed-batch mode. Metab Eng 14(2):91-103.
27. Scalcinati G, Partow S, Siewers V, Schalk M, Daviet L, Nielsen J. 2012b. Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae. Microb Cell Fact 11:1-16.
28. Tippmann S, Chen Y, Siewers V, Nielsen J. 2013. From flavors and pharmaceuticals to advanced biofuels: Production of isoprenoids in Saccharomyces cerevisiae. Biotechnol J 8(12):1435-1444.
29. Tippmann S, Nielsen J, Khoomrung S. 2015. Improved quantification of farnesene during microbial production from S. cerevisiae in two-liquid-phase fermentations. Submitted.
30. Verduyn C. 1991. Physiology of yeasts in relation to biomass yields. Antonie Van Leeuwenhoek 60(3-4):325-353.
31. Verduyn C, Postma E, Scheffers WA, van Dijken JP. 1992. Effect of benzoic acid on metabolic fluxes in yeasts: A continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 8(7):501-517.
32. Villadsen J, Patil KR. 2007. Optimal fed-batch cultivation when mass transfer becomes limiting. Biotechnol Bioeng 98(3):706-710.
33. Wang C, Yoon SH, Jang HJ, Chung YR, Kim JY, Choi ES, Kim SW. 2011. Metabolic engineering of Escherichia coli for α-farnesene production. Metab Eng 13(6):648-655.
34. Xie X, Kirby J, Keasling JD. 2012. Functional characterization of four sesquiterpene synthases from Ricinus communis (Castor bean). Phytochemistry 78:20-28.